Anserine is expressed in human cardiac and skeletal muscles
Abstract We evaluated whether anserine, a methylated analog of the dipeptide carnosine, is present in the cardiac and skeletal muscles of humans and whether the CARNMT1 gene, which encodes the anserine synthesizing enzyme carnosine‐N‐methyltransferase, is expressed in human skeletal muscle. We found...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-10-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.15833 |
| _version_ | 1797397434622541824 |
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| author | Lívia deSouza Gonçalves Wagner Ribeiro Pereira Rafael Pires daSilva Guilherme Carvalho Yamaguchi Victor Henrique Carvalho Bianca Scigliano Vargas Leonardo Jensen Marisa Helena Gennari deMedeiros Hamilton Roschel Guilherme Giannini Artioli |
| author_facet | Lívia deSouza Gonçalves Wagner Ribeiro Pereira Rafael Pires daSilva Guilherme Carvalho Yamaguchi Victor Henrique Carvalho Bianca Scigliano Vargas Leonardo Jensen Marisa Helena Gennari deMedeiros Hamilton Roschel Guilherme Giannini Artioli |
| author_sort | Lívia deSouza Gonçalves |
| collection | DOAJ |
| description | Abstract We evaluated whether anserine, a methylated analog of the dipeptide carnosine, is present in the cardiac and skeletal muscles of humans and whether the CARNMT1 gene, which encodes the anserine synthesizing enzyme carnosine‐N‐methyltransferase, is expressed in human skeletal muscle. We found that anserine is present at low concentrations (low micromolar range) in both cardiac and skeletal muscles, and that anserine content in skeletal muscle is ~15 times higher than in cardiac muscle (cardiac muscle: 10.1 ± 13.4 μmol·kg−1 of dry muscle, n = 12; skeletal muscle: 158.1 ± 68.5 μmol·kg−1 of dry muscle, n = 11, p < 0.0001). Anserine content in the heart was highly variable between individuals, ranging from 1.4 to 45.4 μmol·kg−1 of dry muscle, but anserine content was not associated with sex, age, or body mass. We also showed that CARNMT1 gene is poorly expressed in skeletal muscle (n = 10). This is the first study to demonstrate that anserine is present in the ventricle of the human heart. The presence of anserine in human heart and the confirmation of its expression in human skeletal muscle open new avenues of investigation on the specific and differential physiological functions of histidine dipeptides in striated muscles. |
| first_indexed | 2024-03-09T01:10:53Z |
| format | Article |
| id | doaj.art-b4757f1438b64c8d99d12b9d2c446a75 |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-03-09T01:10:53Z |
| publishDate | 2023-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-b4757f1438b64c8d99d12b9d2c446a752023-12-11T07:16:19ZengWileyPhysiological Reports2051-817X2023-10-011119n/an/a10.14814/phy2.15833Anserine is expressed in human cardiac and skeletal musclesLívia deSouza Gonçalves0Wagner Ribeiro Pereira1Rafael Pires daSilva2Guilherme Carvalho Yamaguchi3Victor Henrique Carvalho4Bianca Scigliano Vargas5Leonardo Jensen6Marisa Helena Gennari deMedeiros7Hamilton Roschel8Guilherme Giannini Artioli9Applied Physiology & Nutrition Research Group—Center of Lifestyle, Faculdade de Medicina Universidade de São Paulo São Paulo BrazilApplied Physiology & Nutrition Research Group—Center of Lifestyle, Faculdade de Medicina Universidade de São Paulo São Paulo BrazilApplied Physiology & Nutrition Research Group—Center of Lifestyle, Faculdade de Medicina Universidade de São Paulo São Paulo BrazilApplied Physiology & Nutrition Research Group—Center of Lifestyle, Faculdade de Medicina Universidade de São Paulo São Paulo BrazilDepartamento de Bioquímica, Instituto de Química Universidade de São Paulo São Paulo BrasilDepartamento de Bioquímica, Instituto de Química Universidade de São Paulo São Paulo BrasilLaboratorio de Hipertensao do Instituto do Coraçao do Hospital das Clínicas da Faculdade de Medicina da Universidade São Paulo São Paulo BrazilDepartamento de Bioquímica, Instituto de Química Universidade de São Paulo São Paulo BrasilApplied Physiology & Nutrition Research Group—Center of Lifestyle, Faculdade de Medicina Universidade de São Paulo São Paulo BrazilDepartment of Life Sciences Manchester Metropolitan University Manchester UKAbstract We evaluated whether anserine, a methylated analog of the dipeptide carnosine, is present in the cardiac and skeletal muscles of humans and whether the CARNMT1 gene, which encodes the anserine synthesizing enzyme carnosine‐N‐methyltransferase, is expressed in human skeletal muscle. We found that anserine is present at low concentrations (low micromolar range) in both cardiac and skeletal muscles, and that anserine content in skeletal muscle is ~15 times higher than in cardiac muscle (cardiac muscle: 10.1 ± 13.4 μmol·kg−1 of dry muscle, n = 12; skeletal muscle: 158.1 ± 68.5 μmol·kg−1 of dry muscle, n = 11, p < 0.0001). Anserine content in the heart was highly variable between individuals, ranging from 1.4 to 45.4 μmol·kg−1 of dry muscle, but anserine content was not associated with sex, age, or body mass. We also showed that CARNMT1 gene is poorly expressed in skeletal muscle (n = 10). This is the first study to demonstrate that anserine is present in the ventricle of the human heart. The presence of anserine in human heart and the confirmation of its expression in human skeletal muscle open new avenues of investigation on the specific and differential physiological functions of histidine dipeptides in striated muscles.https://doi.org/10.14814/phy2.15833anserinecarnosine‐N‐methyltransferasehearthumanmuscle |
| spellingShingle | Lívia deSouza Gonçalves Wagner Ribeiro Pereira Rafael Pires daSilva Guilherme Carvalho Yamaguchi Victor Henrique Carvalho Bianca Scigliano Vargas Leonardo Jensen Marisa Helena Gennari deMedeiros Hamilton Roschel Guilherme Giannini Artioli Anserine is expressed in human cardiac and skeletal muscles Physiological Reports anserine carnosine‐N‐methyltransferase heart human muscle |
| title | Anserine is expressed in human cardiac and skeletal muscles |
| title_full | Anserine is expressed in human cardiac and skeletal muscles |
| title_fullStr | Anserine is expressed in human cardiac and skeletal muscles |
| title_full_unstemmed | Anserine is expressed in human cardiac and skeletal muscles |
| title_short | Anserine is expressed in human cardiac and skeletal muscles |
| title_sort | anserine is expressed in human cardiac and skeletal muscles |
| topic | anserine carnosine‐N‐methyltransferase heart human muscle |
| url | https://doi.org/10.14814/phy2.15833 |
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