Enhanced-Precision Measurement of Glutathionyl Hemoglobin by MALDI-ToF MS
Glutathionyl-hemoglobin (HbSSG) is used as a human biomarker to pinpoint systemic oxidative stress caused by various pathological conditions, noxious lifestyles, and exposure to drugs and environmental or workplace toxicants. Measurement by MALDI mass spectrometry is most frequently used, however, t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/2/497 |
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| author | Federico Maria Rubino Sara Ottolenghi Andrea Brizzolari Claudio Maioli Michele Samaja Rita Paroni |
| author_facet | Federico Maria Rubino Sara Ottolenghi Andrea Brizzolari Claudio Maioli Michele Samaja Rita Paroni |
| author_sort | Federico Maria Rubino |
| collection | DOAJ |
| description | Glutathionyl-hemoglobin (HbSSG) is used as a human biomarker to pinpoint systemic oxidative stress caused by various pathological conditions, noxious lifestyles, and exposure to drugs and environmental or workplace toxicants. Measurement by MALDI mass spectrometry is most frequently used, however, the method suffers from excessive uncontrolled variability. This article describes the improvement of a MALDI-ToF mass spectrometry method for HbSSG measurement through enhanced precision, based on strict control of sample preparation steps and spreadsheet-based data analysis. This improved method displays enhanced precision in the analysis of several hundred samples deriving from studies in different classes of healthy and diseased human subjects. Levels span from 0.5% (lower limit of detection) up to 30%, measured with a precision (as SE%) < 0.5%. We optimized this global procedure to improve data quality and to enable the Operator to work with a reduced physical and psychological strain. Application of this method, for which full instruction and the data analysis spreadsheet are supplied, can encourage the exploitation of HbSSG to study human oxidative stress in a variety of pathological and living conditions and to rationally test the efficacy of antioxidant measures and treatments in the frame of health promotion. |
| first_indexed | 2024-03-09T11:37:27Z |
| format | Article |
| id | doaj.art-b475aeb8a4134892b1e9c2b9cfaef874 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T11:37:27Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b475aeb8a4134892b1e9c2b9cfaef8742023-11-30T23:40:42ZengMDPI AGMolecules1420-30492023-01-0128249710.3390/molecules28020497Enhanced-Precision Measurement of Glutathionyl Hemoglobin by MALDI-ToF MSFederico Maria Rubino0Sara Ottolenghi1Andrea Brizzolari2Claudio Maioli3Michele Samaja4Rita Paroni5Laboratory for Analytical Toxicology and Metabonomics, Department of Health Sciences, Università degli Studi di Milano, v. A. di Rudinì 8, 20142 Milan, ItalyDepartment of Health Sciences, Università degli Studi di Milano, 20146 Milan, ItalyDAN Europe Research Division, 64026 Roseto degli Abruzzi, ItalyDepartment of Health Sciences, Università degli Studi di Milano, 20146 Milan, ItalyDepartment of Health Sciences, Università degli Studi di Milano, 20146 Milan, ItalyLaboratory for Medical Mass Spectrometry, Department of Health Sciences, Università degli Studi di Milano, 20146 Milan, ItalyGlutathionyl-hemoglobin (HbSSG) is used as a human biomarker to pinpoint systemic oxidative stress caused by various pathological conditions, noxious lifestyles, and exposure to drugs and environmental or workplace toxicants. Measurement by MALDI mass spectrometry is most frequently used, however, the method suffers from excessive uncontrolled variability. This article describes the improvement of a MALDI-ToF mass spectrometry method for HbSSG measurement through enhanced precision, based on strict control of sample preparation steps and spreadsheet-based data analysis. This improved method displays enhanced precision in the analysis of several hundred samples deriving from studies in different classes of healthy and diseased human subjects. Levels span from 0.5% (lower limit of detection) up to 30%, measured with a precision (as SE%) < 0.5%. We optimized this global procedure to improve data quality and to enable the Operator to work with a reduced physical and psychological strain. Application of this method, for which full instruction and the data analysis spreadsheet are supplied, can encourage the exploitation of HbSSG to study human oxidative stress in a variety of pathological and living conditions and to rationally test the efficacy of antioxidant measures and treatments in the frame of health promotion.https://www.mdpi.com/1420-3049/28/2/497glutathionehemoglobinmethod optimizationoxidative stressred blood cellsMALDI-ToF |
| spellingShingle | Federico Maria Rubino Sara Ottolenghi Andrea Brizzolari Claudio Maioli Michele Samaja Rita Paroni Enhanced-Precision Measurement of Glutathionyl Hemoglobin by MALDI-ToF MS Molecules glutathione hemoglobin method optimization oxidative stress red blood cells MALDI-ToF |
| title | Enhanced-Precision Measurement of Glutathionyl Hemoglobin by MALDI-ToF MS |
| title_full | Enhanced-Precision Measurement of Glutathionyl Hemoglobin by MALDI-ToF MS |
| title_fullStr | Enhanced-Precision Measurement of Glutathionyl Hemoglobin by MALDI-ToF MS |
| title_full_unstemmed | Enhanced-Precision Measurement of Glutathionyl Hemoglobin by MALDI-ToF MS |
| title_short | Enhanced-Precision Measurement of Glutathionyl Hemoglobin by MALDI-ToF MS |
| title_sort | enhanced precision measurement of glutathionyl hemoglobin by maldi tof ms |
| topic | glutathione hemoglobin method optimization oxidative stress red blood cells MALDI-ToF |
| url | https://www.mdpi.com/1420-3049/28/2/497 |
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