A Vibrio-based microbial platform for accelerated lignocellulosic sugar conversion
Abstract Background Owing to increasing concerns about climate change and the depletion of fossil fuels, the development of efficient microbial processes for biochemical production from lignocellulosic biomass has been a key issue. Because process efficiency is greatly affected by the inherent metab...
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| Format: | Article |
| Language: | English |
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BMC
2022-05-01
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| Series: | Biotechnology for Biofuels and Bioproducts |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13068-022-02157-3 |
| _version_ | 1817989754803716096 |
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| author | Sunghwa Woo Hyun Gyu Lim Yong Hee Han Sungwoo Park Myung Hyun Noh Dongyeop Baek Jo Hyun Moon Sang Woo Seo Gyoo Yeol Jung |
| author_facet | Sunghwa Woo Hyun Gyu Lim Yong Hee Han Sungwoo Park Myung Hyun Noh Dongyeop Baek Jo Hyun Moon Sang Woo Seo Gyoo Yeol Jung |
| author_sort | Sunghwa Woo |
| collection | DOAJ |
| description | Abstract Background Owing to increasing concerns about climate change and the depletion of fossil fuels, the development of efficient microbial processes for biochemical production from lignocellulosic biomass has been a key issue. Because process efficiency is greatly affected by the inherent metabolic activities of host microorganisms, it is essential to utilize a microorganism that can rapidly convert biomass-derived sugars. Here, we report a novel Vibrio-based microbial platform that can rapidly and simultaneously consume three major lignocellulosic sugars (i.e., glucose, xylose, and arabinose) faster than any previously reported microorganisms. Results The xylose isomerase pathway was constructed in Vibrio sp. dhg, which naturally displays high metabolic activities on glucose and arabinose but lacks xylose catabolism. Subsequent adaptive laboratory evolution significantly improved xylose catabolism of initial strain and led to unprecedently high growth and sugar uptake rate (0.67 h−1 and 2.15 g gdry cell weight −1 h−1, respectively). Furthermore, we achieved co-consumption of the three sugars by deletion of PtsG and introduction of GalP. We validated its superior performance and applicability by demonstrating efficient lactate production with high productivity (1.15 g/L/h) and titer (83 g/L). Conclusions In this study, we developed a Vibrio-based microbial platform with rapid and simultaneous utilization of the three major sugars from lignocellulosic biomass by applying an integrated approach of rational and evolutionary engineering. We believe that the developed strain can be broadly utilized to accelerate the production of diverse biochemicals from lignocellulosic biomass. |
| first_indexed | 2024-04-14T00:49:59Z |
| format | Article |
| id | doaj.art-b475d60a3ad54c51972ccf1bebe0b11f |
| institution | Directory Open Access Journal |
| issn | 2731-3654 |
| language | English |
| last_indexed | 2024-04-14T00:49:59Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj.art-b475d60a3ad54c51972ccf1bebe0b11f2022-12-22T02:21:49ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542022-05-0115111210.1186/s13068-022-02157-3A Vibrio-based microbial platform for accelerated lignocellulosic sugar conversionSunghwa Woo0Hyun Gyu Lim1Yong Hee Han2Sungwoo Park3Myung Hyun Noh4Dongyeop Baek5Jo Hyun Moon6Sang Woo Seo7Gyoo Yeol Jung8Department of Chemical Engineering, Pohang University of Science and TechnologyDepartment of Chemical Engineering, Pohang University of Science and TechnologyInterdisciplinary Program in Bioengineering, Seoul National UniversitySchool of Chemical and Biological Engineering, Seoul National UniversityDepartment of Chemical Engineering, Pohang University of Science and TechnologyDepartment of Chemical Engineering, Pohang University of Science and TechnologyDepartment of Chemical Engineering, Pohang University of Science and TechnologyInterdisciplinary Program in Bioengineering, Seoul National UniversityDepartment of Chemical Engineering, Pohang University of Science and TechnologyAbstract Background Owing to increasing concerns about climate change and the depletion of fossil fuels, the development of efficient microbial processes for biochemical production from lignocellulosic biomass has been a key issue. Because process efficiency is greatly affected by the inherent metabolic activities of host microorganisms, it is essential to utilize a microorganism that can rapidly convert biomass-derived sugars. Here, we report a novel Vibrio-based microbial platform that can rapidly and simultaneously consume three major lignocellulosic sugars (i.e., glucose, xylose, and arabinose) faster than any previously reported microorganisms. Results The xylose isomerase pathway was constructed in Vibrio sp. dhg, which naturally displays high metabolic activities on glucose and arabinose but lacks xylose catabolism. Subsequent adaptive laboratory evolution significantly improved xylose catabolism of initial strain and led to unprecedently high growth and sugar uptake rate (0.67 h−1 and 2.15 g gdry cell weight −1 h−1, respectively). Furthermore, we achieved co-consumption of the three sugars by deletion of PtsG and introduction of GalP. We validated its superior performance and applicability by demonstrating efficient lactate production with high productivity (1.15 g/L/h) and titer (83 g/L). Conclusions In this study, we developed a Vibrio-based microbial platform with rapid and simultaneous utilization of the three major sugars from lignocellulosic biomass by applying an integrated approach of rational and evolutionary engineering. We believe that the developed strain can be broadly utilized to accelerate the production of diverse biochemicals from lignocellulosic biomass.https://doi.org/10.1186/s13068-022-02157-3VibrioLignocellulosic biomassXyloseAdaptive laboratory evolutionCarbon catabolite repressionLactate |
| spellingShingle | Sunghwa Woo Hyun Gyu Lim Yong Hee Han Sungwoo Park Myung Hyun Noh Dongyeop Baek Jo Hyun Moon Sang Woo Seo Gyoo Yeol Jung A Vibrio-based microbial platform for accelerated lignocellulosic sugar conversion Biotechnology for Biofuels and Bioproducts Vibrio Lignocellulosic biomass Xylose Adaptive laboratory evolution Carbon catabolite repression Lactate |
| title | A Vibrio-based microbial platform for accelerated lignocellulosic sugar conversion |
| title_full | A Vibrio-based microbial platform for accelerated lignocellulosic sugar conversion |
| title_fullStr | A Vibrio-based microbial platform for accelerated lignocellulosic sugar conversion |
| title_full_unstemmed | A Vibrio-based microbial platform for accelerated lignocellulosic sugar conversion |
| title_short | A Vibrio-based microbial platform for accelerated lignocellulosic sugar conversion |
| title_sort | vibrio based microbial platform for accelerated lignocellulosic sugar conversion |
| topic | Vibrio Lignocellulosic biomass Xylose Adaptive laboratory evolution Carbon catabolite repression Lactate |
| url | https://doi.org/10.1186/s13068-022-02157-3 |
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