HETEROLOGOUS EXPRESSION OF A CHITINASE GENE FROM AEROMONAS CAVIAEIN PSEUDOMONAS FLUORESCENS
A transcriptional fusion for an Aeromonas caviae chitinase gene was constructed under the control of a constitutive promoter of the kanaraycin resistance gene (PKmR ). The construct was inserted into a medium copy number broad host range plasmid vector to yield recombinant plasmid pAM340, which ha...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SEAMEO, BIOTROP
2011-11-01
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| Series: | Biotropia: The Southeast Asian Journal of Tropical Biology |
| Online Access: | https://journal.biotrop.org/index.php/biotropia/article/view/182 |
| _version_ | 1797758589743398912 |
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| author | ANTONIUS SUWANTO AMARILA MALIK BUDI TJAHJONO ROB HARLING |
| author_facet | ANTONIUS SUWANTO AMARILA MALIK BUDI TJAHJONO ROB HARLING |
| author_sort | ANTONIUS SUWANTO |
| collection | DOAJ |
| description | A transcriptional fusion for an Aeromonas caviae chitinase gene was constructed under the control of a constitutive
promoter of the kanaraycin resistance gene (PKmR
). The construct was inserted into a medium copy number broad host
range plasmid vector to yield recombinant plasmid pAM340, which harbored transcriptional fusion PKmR
- chi. Another
transcriptional fusion, Ptac-chi, in a recombinant plasmid pAM630, was conducted as comparison. Triparental mating of E.
coli carrying the recombinant plasmids with Pseudomotws fluorescens 5100, a phyllosphere bacterium, was performed.
Pseudomonas fluorescens 5100 exconjugants were examined for constitutive expression of chitinase employing a
spectrophotometric assay; they showed stronger chitin degradation activity than Escherichia coli transformants. Using a fungal
antagonism plate assay, this chitinolytic P. fluorescens, however, could not inhibit selected phytopathogenic fungi.
Keywords:   Aeromonas  caviae/ chitinase  gene/transcriptional  fusion/PKm'V  Vtac-chilPseudomonas fluorescens |
| first_indexed | 2024-03-12T18:32:12Z |
| format | Article |
| id | doaj.art-b4774f11064c4d42bee59afb696ac878 |
| institution | Directory Open Access Journal |
| issn | 0215-6334 1907-770X |
| language | English |
| last_indexed | 2024-03-12T18:32:12Z |
| publishDate | 2011-11-01 |
| publisher | SEAMEO, BIOTROP |
| record_format | Article |
| series | Biotropia: The Southeast Asian Journal of Tropical Biology |
| spelling | doaj.art-b4774f11064c4d42bee59afb696ac8782023-08-02T08:16:16ZengSEAMEO, BIOTROPBiotropia: The Southeast Asian Journal of Tropical Biology0215-63341907-770X2011-11-012010.11598/btb.2003.0.20.182HETEROLOGOUS EXPRESSION OF A CHITINASE GENE FROM AEROMONAS CAVIAEIN PSEUDOMONAS FLUORESCENSANTONIUS SUWANTO0AMARILA MALIK1BUDI TJAHJONO2ROB HARLING3Department of Biology, Faculty of Mathematics and Sciences,Southeast Asian Regional Center for Tropical Biology (SEAMEO BIOTROP), Bogor,Department of Plant Pests and Diseases, Faculty of Agriculture, Bogor Agricultural University, Bogor-16144, Indonesia: 'Department of Crop Science. Scottish Agricultural College, Edinburgh EH9 3JG, UK.A transcriptional fusion for an Aeromonas caviae chitinase gene was constructed under the control of a constitutive promoter of the kanaraycin resistance gene (PKmR ). The construct was inserted into a medium copy number broad host range plasmid vector to yield recombinant plasmid pAM340, which harbored transcriptional fusion PKmR - chi. Another transcriptional fusion, Ptac-chi, in a recombinant plasmid pAM630, was conducted as comparison. Triparental mating of E. coli carrying the recombinant plasmids with Pseudomotws fluorescens 5100, a phyllosphere bacterium, was performed. Pseudomonas fluorescens 5100 exconjugants were examined for constitutive expression of chitinase employing a spectrophotometric assay; they showed stronger chitin degradation activity than Escherichia coli transformants. Using a fungal antagonism plate assay, this chitinolytic P. fluorescens, however, could not inhibit selected phytopathogenic fungi. Keywords:   Aeromonas  caviae/ chitinase  gene/transcriptional  fusion/PKm'V  Vtac-chilPseudomonas fluorescenshttps://journal.biotrop.org/index.php/biotropia/article/view/182 |
| spellingShingle | ANTONIUS SUWANTO AMARILA MALIK BUDI TJAHJONO ROB HARLING HETEROLOGOUS EXPRESSION OF A CHITINASE GENE FROM AEROMONAS CAVIAEIN PSEUDOMONAS FLUORESCENS Biotropia: The Southeast Asian Journal of Tropical Biology |
| title | HETEROLOGOUS EXPRESSION OF A CHITINASE GENE FROM AEROMONAS CAVIAEIN PSEUDOMONAS FLUORESCENS |
| title_full | HETEROLOGOUS EXPRESSION OF A CHITINASE GENE FROM AEROMONAS CAVIAEIN PSEUDOMONAS FLUORESCENS |
| title_fullStr | HETEROLOGOUS EXPRESSION OF A CHITINASE GENE FROM AEROMONAS CAVIAEIN PSEUDOMONAS FLUORESCENS |
| title_full_unstemmed | HETEROLOGOUS EXPRESSION OF A CHITINASE GENE FROM AEROMONAS CAVIAEIN PSEUDOMONAS FLUORESCENS |
| title_short | HETEROLOGOUS EXPRESSION OF A CHITINASE GENE FROM AEROMONAS CAVIAEIN PSEUDOMONAS FLUORESCENS |
| title_sort | heterologous expression of a chitinase gene from aeromonas caviaein pseudomonas fluorescens |
| url | https://journal.biotrop.org/index.php/biotropia/article/view/182 |
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