Protocol for the establishment and characterization of an endometrial-derived epithelial organoid and stromal cell co-culture system
Summary: Postnatal development of the uterus involves the specification of undifferentiated epithelium into uterine-type epithelium. That specification is regulated by stromal-epithelial interactions as well as intrinsic cell-specific transcription factors and gene regulatory networks. Here, we pres...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724000595 |
| _version_ | 1797304429592969216 |
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| author | Jason A. Rizo Thomas E. Spencer Andrew M. Kelleher |
| author_facet | Jason A. Rizo Thomas E. Spencer Andrew M. Kelleher |
| author_sort | Jason A. Rizo |
| collection | DOAJ |
| description | Summary: Postnatal development of the uterus involves the specification of undifferentiated epithelium into uterine-type epithelium. That specification is regulated by stromal-epithelial interactions as well as intrinsic cell-specific transcription factors and gene regulatory networks. Here, we present a co-culture system to study the effects of stromal-derived factors on epithelial cell growth and differentiation into organoids. First, we describe epithelial cell isolation and organoid growth characterization. Second, we detail a co-culture system that allows the study of stromal-derived paracrine factors on epithelial development.For complete details on the use and execution of this protocol, please refer to Rizo et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-08T00:09:26Z |
| format | Article |
| id | doaj.art-b477bb34099a420ea527527675ae70f4 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-08T00:09:26Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-b477bb34099a420ea527527675ae70f42024-02-17T06:42:18ZengElsevierSTAR Protocols2666-16672024-03-0151102894Protocol for the establishment and characterization of an endometrial-derived epithelial organoid and stromal cell co-culture systemJason A. Rizo0Thomas E. Spencer1Andrew M. Kelleher2Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA; Corresponding authorDivision of Animal Sciences, University of Missouri, Columbia, MO 65211, USA; Department of Obstetrics, Gynecology, and Women’s Health, University of Missouri, Columbia, MO 65211, USADepartment of Obstetrics, Gynecology, and Women’s Health, University of Missouri, Columbia, MO 65211, USA; Corresponding authorSummary: Postnatal development of the uterus involves the specification of undifferentiated epithelium into uterine-type epithelium. That specification is regulated by stromal-epithelial interactions as well as intrinsic cell-specific transcription factors and gene regulatory networks. Here, we present a co-culture system to study the effects of stromal-derived factors on epithelial cell growth and differentiation into organoids. First, we describe epithelial cell isolation and organoid growth characterization. Second, we detail a co-culture system that allows the study of stromal-derived paracrine factors on epithelial development.For complete details on the use and execution of this protocol, please refer to Rizo et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724000595Cell cultureCell isolationDevelopmental biologyModel OrganismsOrganoids |
| spellingShingle | Jason A. Rizo Thomas E. Spencer Andrew M. Kelleher Protocol for the establishment and characterization of an endometrial-derived epithelial organoid and stromal cell co-culture system STAR Protocols Cell culture Cell isolation Developmental biology Model Organisms Organoids |
| title | Protocol for the establishment and characterization of an endometrial-derived epithelial organoid and stromal cell co-culture system |
| title_full | Protocol for the establishment and characterization of an endometrial-derived epithelial organoid and stromal cell co-culture system |
| title_fullStr | Protocol for the establishment and characterization of an endometrial-derived epithelial organoid and stromal cell co-culture system |
| title_full_unstemmed | Protocol for the establishment and characterization of an endometrial-derived epithelial organoid and stromal cell co-culture system |
| title_short | Protocol for the establishment and characterization of an endometrial-derived epithelial organoid and stromal cell co-culture system |
| title_sort | protocol for the establishment and characterization of an endometrial derived epithelial organoid and stromal cell co culture system |
| topic | Cell culture Cell isolation Developmental biology Model Organisms Organoids |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724000595 |
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