In vitro propagation of European larch (Larix decidua Mill.)
Methods of in vitro propagation of Larix decidua are described in the paper. The influence of explant source, tree age, genotype, composition of nutrient media and phytohormones on in vitro propagation of Larix decidua has been investigated. Needles, isolated vegetative buds, shoot tips, zygotic emb...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2004-12-01
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| Series: | Journal of Forest Science |
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| Online Access: | https://jfs.agriculturejournals.cz/artkey/jfs-200412-0001_in-vitro-propagation-of-european-larch-larix-decidua-mill.php |
| _version_ | 1797898933965422592 |
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| author | V. Chalupa |
| author_facet | V. Chalupa |
| author_sort | V. Chalupa |
| collection | DOAJ |
| description | Methods of in vitro propagation of Larix decidua are described in the paper. The influence of explant source, tree age, genotype, composition of nutrient media and phytohormones on in vitro propagation of Larix decidua has been investigated. Needles, isolated vegetative buds, shoot tips, zygotic embryos and cotyledons were used as initial explants. Axillary and adventitious bud cultures were used for fast in vitro shoot multiplication. Root induction was stimulated on low salt medium supplemented with auxin. After rooting and hardening off, micropropagated trees were planted in experimental plots. The growth of micropropagated trees was comparable with the height and diameter growth of trees originated from seeds. |
| first_indexed | 2024-04-10T08:22:02Z |
| format | Article |
| id | doaj.art-b4794e88efa048a2a077c19a4b7924a9 |
| institution | Directory Open Access Journal |
| issn | 1212-4834 1805-935X |
| language | English |
| last_indexed | 2024-04-10T08:22:02Z |
| publishDate | 2004-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Journal of Forest Science |
| spelling | doaj.art-b4794e88efa048a2a077c19a4b7924a92023-02-23T03:41:36ZengCzech Academy of Agricultural SciencesJournal of Forest Science1212-48341805-935X2004-12-01501255355810.17221/4656-JFSjfs-200412-0001In vitro propagation of European larch (Larix decidua Mill.)V. Chalupa0Faculty of Forestry and Environment, Czech University of Agriculture, Prague, Czech RepublicMethods of in vitro propagation of Larix decidua are described in the paper. The influence of explant source, tree age, genotype, composition of nutrient media and phytohormones on in vitro propagation of Larix decidua has been investigated. Needles, isolated vegetative buds, shoot tips, zygotic embryos and cotyledons were used as initial explants. Axillary and adventitious bud cultures were used for fast in vitro shoot multiplication. Root induction was stimulated on low salt medium supplemented with auxin. After rooting and hardening off, micropropagated trees were planted in experimental plots. The growth of micropropagated trees was comparable with the height and diameter growth of trees originated from seeds.https://jfs.agriculturejournals.cz/artkey/jfs-200412-0001_in-vitro-propagation-of-european-larch-larix-decidua-mill.phplarix deciduain vitro propagationlarch micropropagationshoot multiplicationroot inductionfield growth of micropropagated trees |
| spellingShingle | V. Chalupa In vitro propagation of European larch (Larix decidua Mill.) Journal of Forest Science larix decidua in vitro propagation larch micropropagation shoot multiplication root induction field growth of micropropagated trees |
| title | In vitro propagation of European larch (Larix decidua Mill.) |
| title_full | In vitro propagation of European larch (Larix decidua Mill.) |
| title_fullStr | In vitro propagation of European larch (Larix decidua Mill.) |
| title_full_unstemmed | In vitro propagation of European larch (Larix decidua Mill.) |
| title_short | In vitro propagation of European larch (Larix decidua Mill.) |
| title_sort | in vitro propagation of european larch larix decidua mill |
| topic | larix decidua in vitro propagation larch micropropagation shoot multiplication root induction field growth of micropropagated trees |
| url | https://jfs.agriculturejournals.cz/artkey/jfs-200412-0001_in-vitro-propagation-of-european-larch-larix-decidua-mill.php |
| work_keys_str_mv | AT vchalupa invitropropagationofeuropeanlarchlarixdeciduamill |