Phytochemical Analysis, Antibacterial and Antibiofilm Activities of <i>Aloe vera</i> Aqueous Extract against Selected Resistant Gram-Negative Bacteria Involved in Urinary Tract Infections
In bacterial infections, including urinary tract infections (UTIs), the gap between the development of new antimicrobials and antimicrobial resistance is dramatically increasing, especially in Gram-negative (Gram–) bacteria. All healthy products that can be used per se or that may be sources of anti...
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MDPI AG
2022-11-01
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| Series: | Fermentation |
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| author | Mbarga M. J. Arsene Podoprigora I. Viktorovna Goriainov V. Sergei Fadi Hajjar Yashina N. Vyacheslavovna Zhigunova A. Vladimirovna Vasilyeva E. Aleksandrovna Senyagin A. Nikolayevich Nadezhda Sachivkina |
| author_facet | Mbarga M. J. Arsene Podoprigora I. Viktorovna Goriainov V. Sergei Fadi Hajjar Yashina N. Vyacheslavovna Zhigunova A. Vladimirovna Vasilyeva E. Aleksandrovna Senyagin A. Nikolayevich Nadezhda Sachivkina |
| author_sort | Mbarga M. J. Arsene |
| collection | DOAJ |
| description | In bacterial infections, including urinary tract infections (UTIs), the gap between the development of new antimicrobials and antimicrobial resistance is dramatically increasing, especially in Gram-negative (Gram–) bacteria. All healthy products that can be used per se or that may be sources of antibacterial compounds should be considered in the fight against this major public health threat. In the present study, the phytochemical composition of <i>Aloe vera</i> extract was investigated by HPLC–MS/MS, and we further evaluated its antibacterial and antibiofilm formation activity against selected resistant Gram– bacteria involved in UTIs, namely, <i>Achromobacter xylosoxidans</i> 4892, <i>Citrobacter freundii</i> 426, <i>Escherichia coli</i> 1449, <i>Klebsiella oxytoca</i> 3003, <i>Moraxella catarrhalis</i> 4222, <i>Morganella morganii</i> 1543, <i>Pseudomonas aeruginosa</i> 3057, and a reference strain <i>E. coli</i> ATCC 25922. Inhibition zones (IZs) of the extract were determined using the well diffusion method, minimum inhibitory (MIC), and bactericidal (MBC) concentration by the two-fold serial microdilution assay, and antibiofilm formation activity by the crystal violet attachment assay. Aloe-emodin and its derivatives were the major constituent (75.74%) of <i>A. vera</i> extract, the most important of them being aloesin (30.22%), aloe-emodin-diglucoside (12.58%), and 2′-p-methoxycoumaroylaloeresin B (9.64%). The minerals found in the extract were sulfur (S), silicon (Si), chlorine (Cl), potassium (K), and bromine (Br). Except for the clinical strain <i>E. coli </i>1449, which was totally non-susceptible, <i>A. vera</i> demonstrated noteworthy antibacterial activity with MIC and MBC values ranging from 0.625 to 5 mg/mL and 5 to 10 mg/mL, respectively. <i>A. vera</i> also demonstrated dose-dependent antibacterial effects, and the reference strain <i>E. coli</i> ATCC 25922 was the most susceptible with MIC = 0.625 and IZ = 19 mm at 20 mg/mL. The antibiofilm formation potential of <i>A. vera</i> extract was strong at 2MIC and MIC (93–100% of biofilm formation inhibition), moderate at MIC/2 (32–41%), weak at MIC/4 (14–21%), and nil at MIC/8. |
| first_indexed | 2024-03-09T18:20:54Z |
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| last_indexed | 2024-03-09T18:20:54Z |
| publishDate | 2022-11-01 |
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| series | Fermentation |
| spelling | doaj.art-b47caae6dee5400385350dd9350c73752023-11-24T08:19:32ZengMDPI AGFermentation2311-56372022-11-0181162610.3390/fermentation8110626Phytochemical Analysis, Antibacterial and Antibiofilm Activities of <i>Aloe vera</i> Aqueous Extract against Selected Resistant Gram-Negative Bacteria Involved in Urinary Tract InfectionsMbarga M. J. Arsene0Podoprigora I. Viktorovna1Goriainov V. Sergei2Fadi Hajjar3Yashina N. Vyacheslavovna4Zhigunova A. Vladimirovna5Vasilyeva E. Aleksandrovna6Senyagin A. Nikolayevich7Nadezhda Sachivkina8Department of Microbiology V.S. Kiktenko, Peoples Friendship University of Russia (RUDN University), 117198 Moscow, RussiaDepartment of Microbiology V.S. Kiktenko, Peoples Friendship University of Russia (RUDN University), 117198 Moscow, RussiaResearch Institute of Molecular and Cellular Medicine, Рeoples’ Friendship University of Russia (RUDN University), 117198 Moscow, RussiaResearch and Educational Resourse Center Pharmacy, Peoples Friendship University of Russia (RUDN University), 117198 Moscow, RussiaDepartment of Microbiology V.S. Kiktenko, Peoples Friendship University of Russia (RUDN University), 117198 Moscow, RussiaDepartment of Microbiology V.S. Kiktenko, Peoples Friendship University of Russia (RUDN University), 117198 Moscow, RussiaDepartment of Microbiology V.S. Kiktenko, Peoples Friendship University of Russia (RUDN University), 117198 Moscow, RussiaDepartment of Microbiology V.S. Kiktenko, Peoples Friendship University of Russia (RUDN University), 117198 Moscow, RussiaDepartment of Microbiology V.S. Kiktenko, Peoples Friendship University of Russia (RUDN University), 117198 Moscow, RussiaIn bacterial infections, including urinary tract infections (UTIs), the gap between the development of new antimicrobials and antimicrobial resistance is dramatically increasing, especially in Gram-negative (Gram–) bacteria. All healthy products that can be used per se or that may be sources of antibacterial compounds should be considered in the fight against this major public health threat. In the present study, the phytochemical composition of <i>Aloe vera</i> extract was investigated by HPLC–MS/MS, and we further evaluated its antibacterial and antibiofilm formation activity against selected resistant Gram– bacteria involved in UTIs, namely, <i>Achromobacter xylosoxidans</i> 4892, <i>Citrobacter freundii</i> 426, <i>Escherichia coli</i> 1449, <i>Klebsiella oxytoca</i> 3003, <i>Moraxella catarrhalis</i> 4222, <i>Morganella morganii</i> 1543, <i>Pseudomonas aeruginosa</i> 3057, and a reference strain <i>E. coli</i> ATCC 25922. Inhibition zones (IZs) of the extract were determined using the well diffusion method, minimum inhibitory (MIC), and bactericidal (MBC) concentration by the two-fold serial microdilution assay, and antibiofilm formation activity by the crystal violet attachment assay. Aloe-emodin and its derivatives were the major constituent (75.74%) of <i>A. vera</i> extract, the most important of them being aloesin (30.22%), aloe-emodin-diglucoside (12.58%), and 2′-p-methoxycoumaroylaloeresin B (9.64%). The minerals found in the extract were sulfur (S), silicon (Si), chlorine (Cl), potassium (K), and bromine (Br). Except for the clinical strain <i>E. coli </i>1449, which was totally non-susceptible, <i>A. vera</i> demonstrated noteworthy antibacterial activity with MIC and MBC values ranging from 0.625 to 5 mg/mL and 5 to 10 mg/mL, respectively. <i>A. vera</i> also demonstrated dose-dependent antibacterial effects, and the reference strain <i>E. coli</i> ATCC 25922 was the most susceptible with MIC = 0.625 and IZ = 19 mm at 20 mg/mL. The antibiofilm formation potential of <i>A. vera</i> extract was strong at 2MIC and MIC (93–100% of biofilm formation inhibition), moderate at MIC/2 (32–41%), weak at MIC/4 (14–21%), and nil at MIC/8.https://www.mdpi.com/2311-5637/8/11/626antibiotic resistanceGram-negativeantimicrobialurinary tract infections (UTIs)<i>Aloe vera</i> |
| spellingShingle | Mbarga M. J. Arsene Podoprigora I. Viktorovna Goriainov V. Sergei Fadi Hajjar Yashina N. Vyacheslavovna Zhigunova A. Vladimirovna Vasilyeva E. Aleksandrovna Senyagin A. Nikolayevich Nadezhda Sachivkina Phytochemical Analysis, Antibacterial and Antibiofilm Activities of <i>Aloe vera</i> Aqueous Extract against Selected Resistant Gram-Negative Bacteria Involved in Urinary Tract Infections Fermentation antibiotic resistance Gram-negative antimicrobial urinary tract infections (UTIs) <i>Aloe vera</i> |
| title | Phytochemical Analysis, Antibacterial and Antibiofilm Activities of <i>Aloe vera</i> Aqueous Extract against Selected Resistant Gram-Negative Bacteria Involved in Urinary Tract Infections |
| title_full | Phytochemical Analysis, Antibacterial and Antibiofilm Activities of <i>Aloe vera</i> Aqueous Extract against Selected Resistant Gram-Negative Bacteria Involved in Urinary Tract Infections |
| title_fullStr | Phytochemical Analysis, Antibacterial and Antibiofilm Activities of <i>Aloe vera</i> Aqueous Extract against Selected Resistant Gram-Negative Bacteria Involved in Urinary Tract Infections |
| title_full_unstemmed | Phytochemical Analysis, Antibacterial and Antibiofilm Activities of <i>Aloe vera</i> Aqueous Extract against Selected Resistant Gram-Negative Bacteria Involved in Urinary Tract Infections |
| title_short | Phytochemical Analysis, Antibacterial and Antibiofilm Activities of <i>Aloe vera</i> Aqueous Extract against Selected Resistant Gram-Negative Bacteria Involved in Urinary Tract Infections |
| title_sort | phytochemical analysis antibacterial and antibiofilm activities of i aloe vera i aqueous extract against selected resistant gram negative bacteria involved in urinary tract infections |
| topic | antibiotic resistance Gram-negative antimicrobial urinary tract infections (UTIs) <i>Aloe vera</i> |
| url | https://www.mdpi.com/2311-5637/8/11/626 |
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