In-depth proteomics analysis of sentinel lymph nodes from individuals with endometrial cancer
Summary: Endometrial cancer (EC) is one of the most common gynecological cancers worldwide. Sentinel lymph node (SLN) status could be a major prognostic factor in evaluation of EC, but several prospective studies need to be performed. Here we report an in-depth proteomics analysis showing significan...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | Cell Reports Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666379121001610 |
| _version_ | 1818741642600906752 |
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| author | Soulaimane Aboulouard Maxence Wisztorski Marie Duhamel Philippe Saudemont Tristan Cardon Fabrice Narducci Anne-Sophie Lemaire Firas Kobeissy Eric Leblanc Isabelle Fournier Michel Salzet |
| author_facet | Soulaimane Aboulouard Maxence Wisztorski Marie Duhamel Philippe Saudemont Tristan Cardon Fabrice Narducci Anne-Sophie Lemaire Firas Kobeissy Eric Leblanc Isabelle Fournier Michel Salzet |
| author_sort | Soulaimane Aboulouard |
| collection | DOAJ |
| description | Summary: Endometrial cancer (EC) is one of the most common gynecological cancers worldwide. Sentinel lymph node (SLN) status could be a major prognostic factor in evaluation of EC, but several prospective studies need to be performed. Here we report an in-depth proteomics analysis showing significant variations in the SLN protein landscape in EC. We show that SLNs are correlated to each tumor grade, which strengthens evidence of SLN involvement in EC. A few proteins are overexpressed specifically at each EC tumor grade and in the corresponding SLN. These proteins, which are significantly variable in both locations, should be considered potential markers of overall survival. Five major proteins for EC and SLN (PRSS3, PTX3, ASS1, ALDH2, and ANXA1) were identified in large-scale proteomics and validated by immunohistochemistry. This study improves stratification and diagnosis of individuals with EC as a result of proteomics profiling of SLNs. |
| first_indexed | 2024-12-18T01:59:52Z |
| format | Article |
| id | doaj.art-b47cfe60665344049f18363e895cfa87 |
| institution | Directory Open Access Journal |
| issn | 2666-3791 |
| language | English |
| last_indexed | 2024-12-18T01:59:52Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports Medicine |
| spelling | doaj.art-b47cfe60665344049f18363e895cfa872022-12-21T21:24:47ZengElsevierCell Reports Medicine2666-37912021-06-0126100318In-depth proteomics analysis of sentinel lymph nodes from individuals with endometrial cancerSoulaimane Aboulouard0Maxence Wisztorski1Marie Duhamel2Philippe Saudemont3Tristan Cardon4Fabrice Narducci5Anne-Sophie Lemaire6Firas Kobeissy7Eric Leblanc8Isabelle Fournier9Michel Salzet10Université Lille, INSERM, CHU Lille, U1192, Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), 59000 Lille, FranceUniversité Lille, INSERM, CHU Lille, U1192, Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), 59000 Lille, FranceUniversité Lille, INSERM, CHU Lille, U1192, Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), 59000 Lille, FranceUniversité Lille, INSERM, CHU Lille, U1192, Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), 59000 Lille, FranceUniversité Lille, INSERM, CHU Lille, U1192, Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), 59000 Lille, FranceUniversité Lille, INSERM, CHU Lille, U1192, Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), 59000 Lille, France; Department of Gynecology Oncology, Oscar Lambret Center, 59000 Lille, FranceUniversité Lille, INSERM, CHU Lille, U1192, Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), 59000 Lille, France; Department of Gynecology Oncology, Oscar Lambret Center, 59000 Lille, FranceDepartment of Biochemistry and Molecular Genetics, Faculty of Medicine, American University of Beirut, Beirut, LebanonUniversité Lille, INSERM, CHU Lille, U1192, Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), 59000 Lille, France; Department of Gynecology Oncology, Oscar Lambret Center, 59000 Lille, France; Corresponding authorUniversité Lille, INSERM, CHU Lille, U1192, Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), 59000 Lille, France; Corresponding authorUniversité Lille, INSERM, CHU Lille, U1192, Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), 59000 Lille, France; Corresponding authorSummary: Endometrial cancer (EC) is one of the most common gynecological cancers worldwide. Sentinel lymph node (SLN) status could be a major prognostic factor in evaluation of EC, but several prospective studies need to be performed. Here we report an in-depth proteomics analysis showing significant variations in the SLN protein landscape in EC. We show that SLNs are correlated to each tumor grade, which strengthens evidence of SLN involvement in EC. A few proteins are overexpressed specifically at each EC tumor grade and in the corresponding SLN. These proteins, which are significantly variable in both locations, should be considered potential markers of overall survival. Five major proteins for EC and SLN (PRSS3, PTX3, ASS1, ALDH2, and ANXA1) were identified in large-scale proteomics and validated by immunohistochemistry. This study improves stratification and diagnosis of individuals with EC as a result of proteomics profiling of SLNs.http://www.sciencedirect.com/science/article/pii/S2666379121001610sentinel lymph nodesendometrial cancerproteomicsalternative proteinsghost proteomeprotein mutations |
| spellingShingle | Soulaimane Aboulouard Maxence Wisztorski Marie Duhamel Philippe Saudemont Tristan Cardon Fabrice Narducci Anne-Sophie Lemaire Firas Kobeissy Eric Leblanc Isabelle Fournier Michel Salzet In-depth proteomics analysis of sentinel lymph nodes from individuals with endometrial cancer Cell Reports Medicine sentinel lymph nodes endometrial cancer proteomics alternative proteins ghost proteome protein mutations |
| title | In-depth proteomics analysis of sentinel lymph nodes from individuals with endometrial cancer |
| title_full | In-depth proteomics analysis of sentinel lymph nodes from individuals with endometrial cancer |
| title_fullStr | In-depth proteomics analysis of sentinel lymph nodes from individuals with endometrial cancer |
| title_full_unstemmed | In-depth proteomics analysis of sentinel lymph nodes from individuals with endometrial cancer |
| title_short | In-depth proteomics analysis of sentinel lymph nodes from individuals with endometrial cancer |
| title_sort | in depth proteomics analysis of sentinel lymph nodes from individuals with endometrial cancer |
| topic | sentinel lymph nodes endometrial cancer proteomics alternative proteins ghost proteome protein mutations |
| url | http://www.sciencedirect.com/science/article/pii/S2666379121001610 |
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