IMPROVED CELLULASE PRODUCTION BY Aspergillus terreus USING OIL PALM EMPTY FRUIT BUNCH FIBRE AS SUBSTRATE IN A STIRRED TANK BIOREACTOR THROUGH OPTIMIZATION OF THE FERMENTATION CONDITIONS
Response surface methodology (RSM) was performed to evaluate the effects of dissolved oxygen tension (DOT) and initial pH on the production of carboxymethyl cellulase (CMCase), filter-paper hydrolase (FPase), and β-glucosidase by Aspergillus terreus in a 2 L stirred tank bioreactor. Delignified oil...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
North Carolina State University
2011-05-01
|
| Series: | BioResources |
| Subjects: | |
| Online Access: | http://www.ncsu.edu/bioresources/BioRes_06/BioRes_06_3_2663_Shahriarinour_RAMMA_Cellulase_A_terreus_STR_OPEFB_Substrate_1671.pdf |
| _version_ | 1811281058647244800 |
|---|---|
| author | Mahdi Shahriarinour Ramakrishnan Nagasundara Ramanan Mail Mohd Noor Bin Abdul Wahab Rosfarizan Bin Mohamad Shuhaimi Mustafa Mail Arbakariya Bin Ariff |
| author_facet | Mahdi Shahriarinour Ramakrishnan Nagasundara Ramanan Mail Mohd Noor Bin Abdul Wahab Rosfarizan Bin Mohamad Shuhaimi Mustafa Mail Arbakariya Bin Ariff |
| author_sort | Mahdi Shahriarinour |
| collection | DOAJ |
| description | Response surface methodology (RSM) was performed to evaluate the effects of dissolved oxygen tension (DOT) and initial pH on the production of carboxymethyl cellulase (CMCase), filter-paper hydrolase (FPase), and β-glucosidase by Aspergillus terreus in a 2 L stirred tank bioreactor. Delignified oil palm empty fruit bunch (OPEFB) fibre was used as the main substrate under submerged fermentation. Growth of A. terreus and the production of three main components of cellulase were optimized by central composite design (CCD) design. Statistical analysis of results showed that the individual terms of these two variables (DOT and pH) had significant effects on growth and the production of all components of cellulase. Maximum growth (13.07 g/L) and cellulase activity (CMCase = 50.33 U/mL, FPase = 2.29 U/mL and β-glucosidase = 15.98 U/ml) were obtained when the DOT and initial culture pH were set at 55% and 5.5, respectively. A high proportion of β-glucosidase to FPase (8:1) in cellulase of A. terreus could be beneficial for efficient hydrolysis of cellulosic materials. The use of OPEFB as a main substrate would reduce the cost of fermentation for the production of cellulase. |
| first_indexed | 2024-04-13T01:26:45Z |
| format | Article |
| id | doaj.art-b4801d6de56145c18cff338842ea563b |
| institution | Directory Open Access Journal |
| issn | 1930-2126 |
| language | English |
| last_indexed | 2024-04-13T01:26:45Z |
| publishDate | 2011-05-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-b4801d6de56145c18cff338842ea563b2022-12-22T03:08:37ZengNorth Carolina State UniversityBioResources1930-21262011-05-016326632675IMPROVED CELLULASE PRODUCTION BY Aspergillus terreus USING OIL PALM EMPTY FRUIT BUNCH FIBRE AS SUBSTRATE IN A STIRRED TANK BIOREACTOR THROUGH OPTIMIZATION OF THE FERMENTATION CONDITIONSMahdi ShahriarinourRamakrishnan Nagasundara Ramanan MailMohd Noor Bin Abdul WahabRosfarizan Bin MohamadShuhaimi Mustafa MailArbakariya Bin AriffResponse surface methodology (RSM) was performed to evaluate the effects of dissolved oxygen tension (DOT) and initial pH on the production of carboxymethyl cellulase (CMCase), filter-paper hydrolase (FPase), and β-glucosidase by Aspergillus terreus in a 2 L stirred tank bioreactor. Delignified oil palm empty fruit bunch (OPEFB) fibre was used as the main substrate under submerged fermentation. Growth of A. terreus and the production of three main components of cellulase were optimized by central composite design (CCD) design. Statistical analysis of results showed that the individual terms of these two variables (DOT and pH) had significant effects on growth and the production of all components of cellulase. Maximum growth (13.07 g/L) and cellulase activity (CMCase = 50.33 U/mL, FPase = 2.29 U/mL and β-glucosidase = 15.98 U/ml) were obtained when the DOT and initial culture pH were set at 55% and 5.5, respectively. A high proportion of β-glucosidase to FPase (8:1) in cellulase of A. terreus could be beneficial for efficient hydrolysis of cellulosic materials. The use of OPEFB as a main substrate would reduce the cost of fermentation for the production of cellulase.http://www.ncsu.edu/bioresources/BioRes_06/BioRes_06_3_2663_Shahriarinour_RAMMA_Cellulase_A_terreus_STR_OPEFB_Substrate_1671.pdfOil palm empty fruit bunchCellulaseAspergillus terreusDissolved oxygen tensionResponse surface methodologySubmerged fermentation |
| spellingShingle | Mahdi Shahriarinour Ramakrishnan Nagasundara Ramanan Mail Mohd Noor Bin Abdul Wahab Rosfarizan Bin Mohamad Shuhaimi Mustafa Mail Arbakariya Bin Ariff IMPROVED CELLULASE PRODUCTION BY Aspergillus terreus USING OIL PALM EMPTY FRUIT BUNCH FIBRE AS SUBSTRATE IN A STIRRED TANK BIOREACTOR THROUGH OPTIMIZATION OF THE FERMENTATION CONDITIONS BioResources Oil palm empty fruit bunch Cellulase Aspergillus terreus Dissolved oxygen tension Response surface methodology Submerged fermentation |
| title | IMPROVED CELLULASE PRODUCTION BY Aspergillus terreus USING OIL PALM EMPTY FRUIT BUNCH FIBRE AS SUBSTRATE IN A STIRRED TANK BIOREACTOR THROUGH OPTIMIZATION OF THE FERMENTATION CONDITIONS |
| title_full | IMPROVED CELLULASE PRODUCTION BY Aspergillus terreus USING OIL PALM EMPTY FRUIT BUNCH FIBRE AS SUBSTRATE IN A STIRRED TANK BIOREACTOR THROUGH OPTIMIZATION OF THE FERMENTATION CONDITIONS |
| title_fullStr | IMPROVED CELLULASE PRODUCTION BY Aspergillus terreus USING OIL PALM EMPTY FRUIT BUNCH FIBRE AS SUBSTRATE IN A STIRRED TANK BIOREACTOR THROUGH OPTIMIZATION OF THE FERMENTATION CONDITIONS |
| title_full_unstemmed | IMPROVED CELLULASE PRODUCTION BY Aspergillus terreus USING OIL PALM EMPTY FRUIT BUNCH FIBRE AS SUBSTRATE IN A STIRRED TANK BIOREACTOR THROUGH OPTIMIZATION OF THE FERMENTATION CONDITIONS |
| title_short | IMPROVED CELLULASE PRODUCTION BY Aspergillus terreus USING OIL PALM EMPTY FRUIT BUNCH FIBRE AS SUBSTRATE IN A STIRRED TANK BIOREACTOR THROUGH OPTIMIZATION OF THE FERMENTATION CONDITIONS |
| title_sort | improved cellulase production by aspergillus terreus using oil palm empty fruit bunch fibre as substrate in a stirred tank bioreactor through optimization of the fermentation conditions |
| topic | Oil palm empty fruit bunch Cellulase Aspergillus terreus Dissolved oxygen tension Response surface methodology Submerged fermentation |
| url | http://www.ncsu.edu/bioresources/BioRes_06/BioRes_06_3_2663_Shahriarinour_RAMMA_Cellulase_A_terreus_STR_OPEFB_Substrate_1671.pdf |
| work_keys_str_mv | AT mahdishahriarinour improvedcellulaseproductionbyaspergillusterreususingoilpalmemptyfruitbunchfibreassubstrateinastirredtankbioreactorthroughoptimizationofthefermentationconditions AT ramakrishnannagasundararamananmail improvedcellulaseproductionbyaspergillusterreususingoilpalmemptyfruitbunchfibreassubstrateinastirredtankbioreactorthroughoptimizationofthefermentationconditions AT mohdnoorbinabdulwahab improvedcellulaseproductionbyaspergillusterreususingoilpalmemptyfruitbunchfibreassubstrateinastirredtankbioreactorthroughoptimizationofthefermentationconditions AT rosfarizanbinmohamad improvedcellulaseproductionbyaspergillusterreususingoilpalmemptyfruitbunchfibreassubstrateinastirredtankbioreactorthroughoptimizationofthefermentationconditions AT shuhaimimustafamail improvedcellulaseproductionbyaspergillusterreususingoilpalmemptyfruitbunchfibreassubstrateinastirredtankbioreactorthroughoptimizationofthefermentationconditions AT arbakariyabinariff improvedcellulaseproductionbyaspergillusterreususingoilpalmemptyfruitbunchfibreassubstrateinastirredtankbioreactorthroughoptimizationofthefermentationconditions |