LC–MS<sup>3</sup> Strategy for Quantification of Carbamazepine in Human Plasma and Its Application in Therapeutic Drug Monitoring
This study developed a detection method based on the strategy of HPLC/MS<sup>3</sup> and verified its suitability by quantifying carbamazepine in human plasma. The high-performance liquid chromatography–tandem mass spectrometry (HPLC/MS<sup>3</sup>) system was performed using...
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MDPI AG
2022-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/4/1224 |
| _version_ | 1797477677021528064 |
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| author | Dongxiao Ma Zhengchao Ji Haiwei Cao Jing Huang Lei Zeng Lei Yin |
| author_facet | Dongxiao Ma Zhengchao Ji Haiwei Cao Jing Huang Lei Zeng Lei Yin |
| author_sort | Dongxiao Ma |
| collection | DOAJ |
| description | This study developed a detection method based on the strategy of HPLC/MS<sup>3</sup> and verified its suitability by quantifying carbamazepine in human plasma. The high-performance liquid chromatography–tandem mass spectrometry (HPLC/MS<sup>3</sup>) system was performed using a Shimadzu UFLC XR liquid chromatography and a SCIEX QTRAP<sup>®</sup> 5500 linear ion trap triple quadrupole mass spectrometer. The specific operation was as follows: the sample protein was firstly precipitated using methanol, then carbamazepine and carbamazepine-D<sub>2</sub>N<sup>15</sup> were separated on an ACQUITY UPLC HSS T3 column using the gradient elution with solvent A (0.1% formic acid) and solvent B (0.1% formic acid in acetonitrile) at a flow rate of 0.25 mL/min. Each sample was run for 7 min. This method was validated for various parameters including accuracy, precision, selectivity, linearity, LLOQ, etc. Only 5 μL of sample plasma could obtain the result of LLOD 0.5 µg/mL. The intra-day and inter-day precision was <8.23%, and accuracy was between −1.74% and 2.92%. This method was successfully used for monitoring the blood concentration of epilepsy patients after carbamazepine treatment. |
| first_indexed | 2024-03-09T21:21:06Z |
| format | Article |
| id | doaj.art-b480501038fe46ebb87ce0e44280de72 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T21:21:06Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b480501038fe46ebb87ce0e44280de722023-11-23T21:20:41ZengMDPI AGMolecules1420-30492022-02-01274122410.3390/molecules27041224LC–MS<sup>3</sup> Strategy for Quantification of Carbamazepine in Human Plasma and Its Application in Therapeutic Drug MonitoringDongxiao Ma0Zhengchao Ji1Haiwei Cao2Jing Huang3Lei Zeng4Lei Yin5Department of Clinical Laboratory, The First Hospital of Jilin University, Jilin University, Changchun 130021, ChinaDepartment of Clinical Laboratory, The First Hospital of Jilin University, Jilin University, Changchun 130021, ChinaDepartment of Clinical Laboratory, The First Hospital of Jilin University, Jilin University, Changchun 130021, ChinaDepartment of Clinical Laboratory, The First Hospital of Jilin University, Jilin University, Changchun 130021, ChinaThe Bethune Institute of Epigenetic Medicine, The First Hospital of Jilin University, Jilin University, Changchun 130021, ChinaSchool of Life and Pharmaceutical Sciences, Dalian University of Technology, Panjin 124221, ChinaThis study developed a detection method based on the strategy of HPLC/MS<sup>3</sup> and verified its suitability by quantifying carbamazepine in human plasma. The high-performance liquid chromatography–tandem mass spectrometry (HPLC/MS<sup>3</sup>) system was performed using a Shimadzu UFLC XR liquid chromatography and a SCIEX QTRAP<sup>®</sup> 5500 linear ion trap triple quadrupole mass spectrometer. The specific operation was as follows: the sample protein was firstly precipitated using methanol, then carbamazepine and carbamazepine-D<sub>2</sub>N<sup>15</sup> were separated on an ACQUITY UPLC HSS T3 column using the gradient elution with solvent A (0.1% formic acid) and solvent B (0.1% formic acid in acetonitrile) at a flow rate of 0.25 mL/min. Each sample was run for 7 min. This method was validated for various parameters including accuracy, precision, selectivity, linearity, LLOQ, etc. Only 5 μL of sample plasma could obtain the result of LLOD 0.5 µg/mL. The intra-day and inter-day precision was <8.23%, and accuracy was between −1.74% and 2.92%. This method was successfully used for monitoring the blood concentration of epilepsy patients after carbamazepine treatment.https://www.mdpi.com/1420-3049/27/4/1224LC-MS<sup>3</sup>carbamazepinetherapeutic drug monitoring |
| spellingShingle | Dongxiao Ma Zhengchao Ji Haiwei Cao Jing Huang Lei Zeng Lei Yin LC–MS<sup>3</sup> Strategy for Quantification of Carbamazepine in Human Plasma and Its Application in Therapeutic Drug Monitoring Molecules LC-MS<sup>3</sup> carbamazepine therapeutic drug monitoring |
| title | LC–MS<sup>3</sup> Strategy for Quantification of Carbamazepine in Human Plasma and Its Application in Therapeutic Drug Monitoring |
| title_full | LC–MS<sup>3</sup> Strategy for Quantification of Carbamazepine in Human Plasma and Its Application in Therapeutic Drug Monitoring |
| title_fullStr | LC–MS<sup>3</sup> Strategy for Quantification of Carbamazepine in Human Plasma and Its Application in Therapeutic Drug Monitoring |
| title_full_unstemmed | LC–MS<sup>3</sup> Strategy for Quantification of Carbamazepine in Human Plasma and Its Application in Therapeutic Drug Monitoring |
| title_short | LC–MS<sup>3</sup> Strategy for Quantification of Carbamazepine in Human Plasma and Its Application in Therapeutic Drug Monitoring |
| title_sort | lc ms sup 3 sup strategy for quantification of carbamazepine in human plasma and its application in therapeutic drug monitoring |
| topic | LC-MS<sup>3</sup> carbamazepine therapeutic drug monitoring |
| url | https://www.mdpi.com/1420-3049/27/4/1224 |
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