Low Micromolar Ba2+ Potentiates Glutamate Transporter Current in Hippocampal Astrocytes
Glutamate uptake, mediated by electrogenic glutamate transporters largely localized in astrocytes, is responsible for the clearance of glutamate released during excitatory synaptic transmission. Glutamate uptake also determines the availability of glutamate for extrasynaptic glutamate receptors. The...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-08-01
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| Series: | Frontiers in Cellular Neuroscience |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncel.2013.00135/full |
| _version_ | 1818624069100109824 |
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| author | Ramil eAfzalov Ramil eAfzalov Evgeny ePryazhnikov Pei-Yu eShih Elena eKondratskaya Svetlana eZobova Sakari eLeino Outi eSalminen Leonard eKhiroug Alexey eSemyanov Alexey eSemyanov |
| author_facet | Ramil eAfzalov Ramil eAfzalov Evgeny ePryazhnikov Pei-Yu eShih Elena eKondratskaya Svetlana eZobova Sakari eLeino Outi eSalminen Leonard eKhiroug Alexey eSemyanov Alexey eSemyanov |
| author_sort | Ramil eAfzalov |
| collection | DOAJ |
| description | Glutamate uptake, mediated by electrogenic glutamate transporters largely localized in astrocytes, is responsible for the clearance of glutamate released during excitatory synaptic transmission. Glutamate uptake also determines the availability of glutamate for extrasynaptic glutamate receptors. The efficiency of glutamate uptake is commonly estimated from the amplitude of transporter current recorded in astrocytes. We recorded currents in voltage-clamped hippocampal CA1 stratum radiatum astrocytes in rat hippocampal slices induced by electrical stimulation of the Schaffer collaterals. A Ba2+-sensitive K+ current mediated by inward rectifying potassium channels (Kir) accompanied the transporter current. Surprisingly, Ba2+ not only suppressed the K+ current and changed holding current (presumably, mediated by Kir) but also increased the transporter current at lower concentrations. However, Ba2+ did not significantly increase the uptake of aspartate in cultured astrocytes, suggesting that increase in the amplitude of the transporter current does not always reflect changes in glutamate uptake. |
| first_indexed | 2024-12-16T18:51:05Z |
| format | Article |
| id | doaj.art-b4821992ef8b41be9118701b9cbc7367 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-16T18:51:05Z |
| publishDate | 2013-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-b4821992ef8b41be9118701b9cbc73672022-12-21T22:20:41ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022013-08-01710.3389/fncel.2013.0013543622Low Micromolar Ba2+ Potentiates Glutamate Transporter Current in Hippocampal AstrocytesRamil eAfzalov0Ramil eAfzalov1Evgeny ePryazhnikov2Pei-Yu eShih3Elena eKondratskaya4Svetlana eZobova5Sakari eLeino6Outi eSalminen7Leonard eKhiroug8Alexey eSemyanov9Alexey eSemyanov10RIKEN Brain Science InstituteUniversity of HelsinkiUniversity of HelsinkiRIKEN Brain Science InstituteRIKEN Brain Science InstituteUniversity of HelsinkiUniversity of HelsinkiUniversity of HelsinkiUniversity of HelsinkiRIKEN Brain Science InstituteUniversity of Nizhny NovgorodGlutamate uptake, mediated by electrogenic glutamate transporters largely localized in astrocytes, is responsible for the clearance of glutamate released during excitatory synaptic transmission. Glutamate uptake also determines the availability of glutamate for extrasynaptic glutamate receptors. The efficiency of glutamate uptake is commonly estimated from the amplitude of transporter current recorded in astrocytes. We recorded currents in voltage-clamped hippocampal CA1 stratum radiatum astrocytes in rat hippocampal slices induced by electrical stimulation of the Schaffer collaterals. A Ba2+-sensitive K+ current mediated by inward rectifying potassium channels (Kir) accompanied the transporter current. Surprisingly, Ba2+ not only suppressed the K+ current and changed holding current (presumably, mediated by Kir) but also increased the transporter current at lower concentrations. However, Ba2+ did not significantly increase the uptake of aspartate in cultured astrocytes, suggesting that increase in the amplitude of the transporter current does not always reflect changes in glutamate uptake.http://journal.frontiersin.org/Journal/10.3389/fncel.2013.00135/fullAstrocytesBariumHippocampusglutamate transportersGlutamate Uptake |
| spellingShingle | Ramil eAfzalov Ramil eAfzalov Evgeny ePryazhnikov Pei-Yu eShih Elena eKondratskaya Svetlana eZobova Sakari eLeino Outi eSalminen Leonard eKhiroug Alexey eSemyanov Alexey eSemyanov Low Micromolar Ba2+ Potentiates Glutamate Transporter Current in Hippocampal Astrocytes Frontiers in Cellular Neuroscience Astrocytes Barium Hippocampus glutamate transporters Glutamate Uptake |
| title | Low Micromolar Ba2+ Potentiates Glutamate Transporter Current in Hippocampal Astrocytes |
| title_full | Low Micromolar Ba2+ Potentiates Glutamate Transporter Current in Hippocampal Astrocytes |
| title_fullStr | Low Micromolar Ba2+ Potentiates Glutamate Transporter Current in Hippocampal Astrocytes |
| title_full_unstemmed | Low Micromolar Ba2+ Potentiates Glutamate Transporter Current in Hippocampal Astrocytes |
| title_short | Low Micromolar Ba2+ Potentiates Glutamate Transporter Current in Hippocampal Astrocytes |
| title_sort | low micromolar ba2 potentiates glutamate transporter current in hippocampal astrocytes |
| topic | Astrocytes Barium Hippocampus glutamate transporters Glutamate Uptake |
| url | http://journal.frontiersin.org/Journal/10.3389/fncel.2013.00135/full |
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