LncRNA GACAT3 promotes esophageal squamous cell carcinoma progression through regulation of miR-149/FOXM1
Abstract Background The long noncoding RNA gastric cancer associated transcript 3 (GACAT3) has been demonstrated to be implicated in the carcinogenesis and progression of many malignancies. However, GACAT3’s levels and role in esophageal squamous cell carcinoma (ESCC) has not been elucidated. Method...
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| Format: | Article |
| Language: | English |
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BMC
2021-09-01
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| Series: | Cancer Cell International |
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| Online Access: | https://doi.org/10.1186/s12935-021-02192-4 |
| _version_ | 1818582739825197056 |
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| author | Min Su Jinming Tang Baihua Zhang Desong Yang Zhining Wu Jie Wu Yong Zhou Qianjin Liao Hui Wang Wenxiang Wang Yuhang Xiao |
| author_facet | Min Su Jinming Tang Baihua Zhang Desong Yang Zhining Wu Jie Wu Yong Zhou Qianjin Liao Hui Wang Wenxiang Wang Yuhang Xiao |
| author_sort | Min Su |
| collection | DOAJ |
| description | Abstract Background The long noncoding RNA gastric cancer associated transcript 3 (GACAT3) has been demonstrated to be implicated in the carcinogenesis and progression of many malignancies. However, GACAT3’s levels and role in esophageal squamous cell carcinoma (ESCC) has not been elucidated. Methods GACAT3 amounts were investigated in ESCC tissues and cell lines by qPCR. Its biological functions were examined by CCK-8 assay, colony formation assay, flow cytometry, wound healing assay, transwell assay, and xenograft model establishment. The relationship between GACAT3 and miR-149 was assessed by dual-luciferase reporter assay. Results GACAT3 amounts were elevated in ESCC tissue and cell specimens. Functional studies showed that GACAT3 silencing reduced the proliferation, migration and invasion of cultured ESCC cells, and decreased tumor growth in mice. Furthermore, GACAT could directly interact with miR-149. In addition, colony formation and invasion assays verified that GACAT3 promotes ESCC tumor progression through miR-149. Moreover, GACAT3 acted as a competing endogenous RNA (ceRNA) to modulate FOXM1 expression. Conclusions These findings indicate that GACAT3 functions as an oncogene by acting as a ceRNA for miR-149 to modulate FOXM1 expression in ESCC, suggesting that GACAT3 might constitute a therapeutic target in ESCC. |
| first_indexed | 2024-12-16T07:54:11Z |
| format | Article |
| id | doaj.art-b4827a32ce49482bb16dfd260dd05c59 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-16T07:54:11Z |
| publishDate | 2021-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-b4827a32ce49482bb16dfd260dd05c592022-12-21T22:38:47ZengBMCCancer Cell International1475-28672021-09-012111910.1186/s12935-021-02192-4LncRNA GACAT3 promotes esophageal squamous cell carcinoma progression through regulation of miR-149/FOXM1Min Su0Jinming Tang1Baihua Zhang2Desong Yang3Zhining Wu4Jie Wu5Yong Zhou6Qianjin Liao7Hui Wang8Wenxiang Wang9Yuhang Xiao10Hunan Clinical Medical Research Center of Accurate Diagnosis and Treatment for Esophageal carcinoma, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityHunan Clinical Medical Research Center of Accurate Diagnosis and Treatment for Esophageal carcinoma, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityHunan Clinical Medical Research Center of Accurate Diagnosis and Treatment for Esophageal carcinoma, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityHunan Clinical Medical Research Center of Accurate Diagnosis and Treatment for Esophageal carcinoma, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityHunan Clinical Medical Research Center of Accurate Diagnosis and Treatment for Esophageal carcinoma, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityHunan Clinical Medical Research Center of Accurate Diagnosis and Treatment for Esophageal carcinoma, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityHunan Clinical Medical Research Center of Accurate Diagnosis and Treatment for Esophageal carcinoma, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityHunan Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityHunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityHunan Clinical Medical Research Center of Accurate Diagnosis and Treatment for Esophageal carcinoma, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityHunan Clinical Medical Research Center of Accurate Diagnosis and Treatment for Esophageal carcinoma, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South UniversityAbstract Background The long noncoding RNA gastric cancer associated transcript 3 (GACAT3) has been demonstrated to be implicated in the carcinogenesis and progression of many malignancies. However, GACAT3’s levels and role in esophageal squamous cell carcinoma (ESCC) has not been elucidated. Methods GACAT3 amounts were investigated in ESCC tissues and cell lines by qPCR. Its biological functions were examined by CCK-8 assay, colony formation assay, flow cytometry, wound healing assay, transwell assay, and xenograft model establishment. The relationship between GACAT3 and miR-149 was assessed by dual-luciferase reporter assay. Results GACAT3 amounts were elevated in ESCC tissue and cell specimens. Functional studies showed that GACAT3 silencing reduced the proliferation, migration and invasion of cultured ESCC cells, and decreased tumor growth in mice. Furthermore, GACAT could directly interact with miR-149. In addition, colony formation and invasion assays verified that GACAT3 promotes ESCC tumor progression through miR-149. Moreover, GACAT3 acted as a competing endogenous RNA (ceRNA) to modulate FOXM1 expression. Conclusions These findings indicate that GACAT3 functions as an oncogene by acting as a ceRNA for miR-149 to modulate FOXM1 expression in ESCC, suggesting that GACAT3 might constitute a therapeutic target in ESCC.https://doi.org/10.1186/s12935-021-02192-4Esophageal squamous cell carcinomaLong noncoding RNAGACAT3miR-149FOXM1 |
| spellingShingle | Min Su Jinming Tang Baihua Zhang Desong Yang Zhining Wu Jie Wu Yong Zhou Qianjin Liao Hui Wang Wenxiang Wang Yuhang Xiao LncRNA GACAT3 promotes esophageal squamous cell carcinoma progression through regulation of miR-149/FOXM1 Cancer Cell International Esophageal squamous cell carcinoma Long noncoding RNA GACAT3 miR-149 FOXM1 |
| title | LncRNA GACAT3 promotes esophageal squamous cell carcinoma progression through regulation of miR-149/FOXM1 |
| title_full | LncRNA GACAT3 promotes esophageal squamous cell carcinoma progression through regulation of miR-149/FOXM1 |
| title_fullStr | LncRNA GACAT3 promotes esophageal squamous cell carcinoma progression through regulation of miR-149/FOXM1 |
| title_full_unstemmed | LncRNA GACAT3 promotes esophageal squamous cell carcinoma progression through regulation of miR-149/FOXM1 |
| title_short | LncRNA GACAT3 promotes esophageal squamous cell carcinoma progression through regulation of miR-149/FOXM1 |
| title_sort | lncrna gacat3 promotes esophageal squamous cell carcinoma progression through regulation of mir 149 foxm1 |
| topic | Esophageal squamous cell carcinoma Long noncoding RNA GACAT3 miR-149 FOXM1 |
| url | https://doi.org/10.1186/s12935-021-02192-4 |
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