Microbial Mechanistic Insights into the Role of Sweet Potato Vine on Improving Health in Chinese Meishan Gilt Model
This study explored the impact of fresh sweet potato vine on the growth as well as the metabolites and colon microbial composition in Chinese Meishan gilt. Twenty Meishan gilts (body weight 30 ± 0.18 kg, n = 10 per treatment) were randomly assigned to a control (CON) or sweet potato vine (SPV) suppl...
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MDPI AG
2019-08-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/9/9/632 |
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| author | Shengyu Xu Pan Zhang Meng Cao Yanpeng Dong Jian Li Yan Lin Lianqiang Che Zhengfeng Fang Bin Feng Yong Zhuo Jianping Wang Zhihua Ren De Wu |
| author_facet | Shengyu Xu Pan Zhang Meng Cao Yanpeng Dong Jian Li Yan Lin Lianqiang Che Zhengfeng Fang Bin Feng Yong Zhuo Jianping Wang Zhihua Ren De Wu |
| author_sort | Shengyu Xu |
| collection | DOAJ |
| description | This study explored the impact of fresh sweet potato vine on the growth as well as the metabolites and colon microbial composition in Chinese Meishan gilt. Twenty Meishan gilts (body weight 30 ± 0.18 kg, n = 10 per treatment) were randomly assigned to a control (CON) or sweet potato vine (SPV) supplementation diet treatment. Gilts were housed in individual stalls. In the SPV treatment, 2 kg fresh sweet potato vine was used instead of 0.18 kg basal diet which provided the same amount of digestive energy and crude protein with the exception of crude fiber (CON, 51.00 g/d vs. SPV, 73.94 g/d) in terms of dry matter intake. Gilts were slaughtered and samples were collected on day 19 after the third estrus cycle. The SPV treatment tended to increase slaughter weight of gilts (<i>p</i> = 0.07); it also increased (<i>p</i> < 0.05) gastrointestinal tract weight and intestinal muscle layer thickness. SPV treatment also decreased (<i>p</i> < 0.05) carcass yield and subcutaneous adipose tissue. The concentration of zonulin and endotoxin in plasma was decreased (<i>p</i> < 0.05) as the gilt consumed the SPV diet. Colonic fecal concentrations of endotoxin, lipocalin-2, and tumor necrosis factor-α (TNF-α) were decreased (<i>p</i> < 0.05), and interleukin-10 (IL-10) was increased (<i>p</i> < 0.05) in the SPV treatment. Butyric acid and acetate concentration in colonic content as well as acetate concentration in caecal content were increased (<i>p</i> < 0.05) in the SPV treatment. Furthermore, the expression of carnitine palmityl transferase (<i>CPT-1</i>) and peroxisome proliferator-activated receptor-α (<i>PPAR-α</i>) in gilt liver in SPV treatment was increased (<i>p</i> < 0.05) in comparison with CON treatment. Meanwhile, the composition of the colon microbes was also altered by SPV; representative changes included an increase in <i>Lactobacillus</i>, <i>Bacteroides</i>, <i>Roseburia</i>, and <i>Lachnospira.</i> These results indicate that gilt fed with sweet potato vine had decreased gut permeability, endotoxin and pro-inflammatory cytokines concentrations; colonic fecal microbiota was also changed, which may be further beneficial to the intestinal health of Chinese Meishan gilt. |
| first_indexed | 2024-12-11T14:32:51Z |
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| spelling | doaj.art-b4855c2511234ba78b1240f9825123182022-12-22T01:02:21ZengMDPI AGAnimals2076-26152019-08-019963210.3390/ani9090632ani9090632Microbial Mechanistic Insights into the Role of Sweet Potato Vine on Improving Health in Chinese Meishan Gilt ModelShengyu Xu0Pan Zhang1Meng Cao2Yanpeng Dong3Jian Li4Yan Lin5Lianqiang Che6Zhengfeng Fang7Bin Feng8Yong Zhuo9Jianping Wang10Zhihua Ren11De Wu12Key Laboratory of Animal Disease-Resistant Nutrition, Ministry of Education; Key Laboratory of Animal Disease-Resistant Nutrition and Feed, Ministry of Agriculture and Rural Affairs; Key Laboratory of Animal Disease-Resistant Nutrition, Sichuan Province; Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Disease-Resistant Nutrition, Ministry of Education; Key Laboratory of Animal Disease-Resistant Nutrition and Feed, Ministry of Agriculture and Rural Affairs; Key Laboratory of Animal Disease-Resistant Nutrition, Sichuan Province; Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Disease-Resistant Nutrition, Ministry of Education; Key Laboratory of Animal Disease-Resistant Nutrition and Feed, Ministry of Agriculture and Rural Affairs; Key Laboratory of Animal Disease-Resistant Nutrition, Sichuan Province; Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Disease-Resistant Nutrition, Ministry of Education; Key Laboratory of Animal Disease-Resistant Nutrition and Feed, Ministry of Agriculture and Rural Affairs; Key Laboratory of Animal Disease-Resistant Nutrition, Sichuan Province; Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Disease-Resistant Nutrition, Ministry of Education; Key Laboratory of Animal Disease-Resistant Nutrition and Feed, Ministry of Agriculture and Rural Affairs; Key Laboratory of Animal Disease-Resistant Nutrition, Sichuan Province; Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Disease-Resistant Nutrition, Ministry of Education; Key Laboratory of Animal Disease-Resistant Nutrition and Feed, Ministry of Agriculture and Rural Affairs; Key Laboratory of Animal Disease-Resistant Nutrition, Sichuan Province; Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Disease-Resistant Nutrition, Ministry of Education; Key Laboratory of Animal Disease-Resistant Nutrition and Feed, Ministry of Agriculture and Rural Affairs; Key Laboratory of Animal Disease-Resistant Nutrition, Sichuan Province; Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Disease-Resistant Nutrition, Ministry of Education; Key Laboratory of Animal Disease-Resistant Nutrition and Feed, Ministry of Agriculture and Rural Affairs; Key Laboratory of Animal Disease-Resistant Nutrition, Sichuan Province; Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Disease-Resistant Nutrition, Ministry of Education; Key Laboratory of Animal Disease-Resistant Nutrition and Feed, Ministry of Agriculture and Rural Affairs; Key Laboratory of Animal Disease-Resistant Nutrition, Sichuan Province; Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Disease-Resistant Nutrition, Ministry of Education; Key Laboratory of Animal Disease-Resistant Nutrition and Feed, Ministry of Agriculture and Rural Affairs; Key Laboratory of Animal Disease-Resistant Nutrition, Sichuan Province; Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Disease-Resistant Nutrition, Ministry of Education; Key Laboratory of Animal Disease-Resistant Nutrition and Feed, Ministry of Agriculture and Rural Affairs; Key Laboratory of Animal Disease-Resistant Nutrition, Sichuan Province; Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Disease-Resistant Nutrition, Ministry of Education; Key Laboratory of Animal Disease-Resistant Nutrition and Feed, Ministry of Agriculture and Rural Affairs; Key Laboratory of Animal Disease-Resistant Nutrition, Sichuan Province; Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaThis study explored the impact of fresh sweet potato vine on the growth as well as the metabolites and colon microbial composition in Chinese Meishan gilt. Twenty Meishan gilts (body weight 30 ± 0.18 kg, n = 10 per treatment) were randomly assigned to a control (CON) or sweet potato vine (SPV) supplementation diet treatment. Gilts were housed in individual stalls. In the SPV treatment, 2 kg fresh sweet potato vine was used instead of 0.18 kg basal diet which provided the same amount of digestive energy and crude protein with the exception of crude fiber (CON, 51.00 g/d vs. SPV, 73.94 g/d) in terms of dry matter intake. Gilts were slaughtered and samples were collected on day 19 after the third estrus cycle. The SPV treatment tended to increase slaughter weight of gilts (<i>p</i> = 0.07); it also increased (<i>p</i> < 0.05) gastrointestinal tract weight and intestinal muscle layer thickness. SPV treatment also decreased (<i>p</i> < 0.05) carcass yield and subcutaneous adipose tissue. The concentration of zonulin and endotoxin in plasma was decreased (<i>p</i> < 0.05) as the gilt consumed the SPV diet. Colonic fecal concentrations of endotoxin, lipocalin-2, and tumor necrosis factor-α (TNF-α) were decreased (<i>p</i> < 0.05), and interleukin-10 (IL-10) was increased (<i>p</i> < 0.05) in the SPV treatment. Butyric acid and acetate concentration in colonic content as well as acetate concentration in caecal content were increased (<i>p</i> < 0.05) in the SPV treatment. Furthermore, the expression of carnitine palmityl transferase (<i>CPT-1</i>) and peroxisome proliferator-activated receptor-α (<i>PPAR-α</i>) in gilt liver in SPV treatment was increased (<i>p</i> < 0.05) in comparison with CON treatment. Meanwhile, the composition of the colon microbes was also altered by SPV; representative changes included an increase in <i>Lactobacillus</i>, <i>Bacteroides</i>, <i>Roseburia</i>, and <i>Lachnospira.</i> These results indicate that gilt fed with sweet potato vine had decreased gut permeability, endotoxin and pro-inflammatory cytokines concentrations; colonic fecal microbiota was also changed, which may be further beneficial to the intestinal health of Chinese Meishan gilt.https://www.mdpi.com/2076-2615/9/9/632Chinese Meishan giltsweet potato vineendotoxinmicrobial compositionintestinal health |
| spellingShingle | Shengyu Xu Pan Zhang Meng Cao Yanpeng Dong Jian Li Yan Lin Lianqiang Che Zhengfeng Fang Bin Feng Yong Zhuo Jianping Wang Zhihua Ren De Wu Microbial Mechanistic Insights into the Role of Sweet Potato Vine on Improving Health in Chinese Meishan Gilt Model Animals Chinese Meishan gilt sweet potato vine endotoxin microbial composition intestinal health |
| title | Microbial Mechanistic Insights into the Role of Sweet Potato Vine on Improving Health in Chinese Meishan Gilt Model |
| title_full | Microbial Mechanistic Insights into the Role of Sweet Potato Vine on Improving Health in Chinese Meishan Gilt Model |
| title_fullStr | Microbial Mechanistic Insights into the Role of Sweet Potato Vine on Improving Health in Chinese Meishan Gilt Model |
| title_full_unstemmed | Microbial Mechanistic Insights into the Role of Sweet Potato Vine on Improving Health in Chinese Meishan Gilt Model |
| title_short | Microbial Mechanistic Insights into the Role of Sweet Potato Vine on Improving Health in Chinese Meishan Gilt Model |
| title_sort | microbial mechanistic insights into the role of sweet potato vine on improving health in chinese meishan gilt model |
| topic | Chinese Meishan gilt sweet potato vine endotoxin microbial composition intestinal health |
| url | https://www.mdpi.com/2076-2615/9/9/632 |
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