Rapid separation of gram quantities of phospholipids from biological membranes by preparative high performance liquid chromatography
We present a detailed description of a rapid preparative high performance liquid chromatography (HPLC) procedure for isolation of large scale quantities (a minimum of 200 mg) of rat liver microsomal and mitochondrial phospholipid classes in 40 min on a 25 X 2.5 cm preparative HPLC column of 7-mu sil...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1987-08-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520386338 |
| _version_ | 1819058214403047424 |
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| author | J S Ellingson R L Zimmerman |
| author_facet | J S Ellingson R L Zimmerman |
| author_sort | J S Ellingson |
| collection | DOAJ |
| description | We present a detailed description of a rapid preparative high performance liquid chromatography (HPLC) procedure for isolation of large scale quantities (a minimum of 200 mg) of rat liver microsomal and mitochondrial phospholipid classes in 40 min on a 25 X 2.5 cm preparative HPLC column of 7-mu silica gel particles using a linear gradient of hexane-isopropanol-water mixtures. A minimum of 1.5 g of phospholipids can be quantitatively separated per day into diphosphatidylglycerol (cardiolipin), phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, sphingomyelin, and a phosphatidylcholine-sphingomyelin fraction. The procedure uses no salts, buffers, acids, or bases, and the isolated phospholipids are suitable for preparing model membranes. |
| first_indexed | 2024-12-21T13:51:39Z |
| format | Article |
| id | doaj.art-b48608c202694511a1adf4586100a8c8 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T13:51:39Z |
| publishDate | 1987-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-b48608c202694511a1adf4586100a8c82022-12-21T19:01:40ZengElsevierJournal of Lipid Research0022-22751987-08-0128810161018Rapid separation of gram quantities of phospholipids from biological membranes by preparative high performance liquid chromatographyJ S Ellingson0R L Zimmerman1Department of Pathology, Jefferson Medical College of Thomas Jefferson University, Philadelphia, PA 19107.Department of Pathology, Jefferson Medical College of Thomas Jefferson University, Philadelphia, PA 19107.We present a detailed description of a rapid preparative high performance liquid chromatography (HPLC) procedure for isolation of large scale quantities (a minimum of 200 mg) of rat liver microsomal and mitochondrial phospholipid classes in 40 min on a 25 X 2.5 cm preparative HPLC column of 7-mu silica gel particles using a linear gradient of hexane-isopropanol-water mixtures. A minimum of 1.5 g of phospholipids can be quantitatively separated per day into diphosphatidylglycerol (cardiolipin), phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, sphingomyelin, and a phosphatidylcholine-sphingomyelin fraction. The procedure uses no salts, buffers, acids, or bases, and the isolated phospholipids are suitable for preparing model membranes.http://www.sciencedirect.com/science/article/pii/S0022227520386338 |
| spellingShingle | J S Ellingson R L Zimmerman Rapid separation of gram quantities of phospholipids from biological membranes by preparative high performance liquid chromatography Journal of Lipid Research |
| title | Rapid separation of gram quantities of phospholipids from biological membranes by preparative high performance liquid chromatography |
| title_full | Rapid separation of gram quantities of phospholipids from biological membranes by preparative high performance liquid chromatography |
| title_fullStr | Rapid separation of gram quantities of phospholipids from biological membranes by preparative high performance liquid chromatography |
| title_full_unstemmed | Rapid separation of gram quantities of phospholipids from biological membranes by preparative high performance liquid chromatography |
| title_short | Rapid separation of gram quantities of phospholipids from biological membranes by preparative high performance liquid chromatography |
| title_sort | rapid separation of gram quantities of phospholipids from biological membranes by preparative high performance liquid chromatography |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520386338 |
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