Bushen Tongluowan promotes chondrocyte proliferation through multi-gene regulation
Traditional Chinese orthopedics has a long history and excellent clinical effects in the treatment of osteoarthritis (OA). Bushen Tongluowan (BSTLW) is a representative prescription based on ''kidney governing bones'' theory in Traditional Chinese medicine. However, the molecular...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
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| Series: | Pharmacological Research - Modern Chinese Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667142522001245 |
| _version_ | 1811178073420201984 |
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| author | Lu Bocheng Zhou Zhenwei Zhang Pengcheng Huang Jianjun Li Qingmiao Zhao Xiaolin Qu Tingli Zheng Qian |
| author_facet | Lu Bocheng Zhou Zhenwei Zhang Pengcheng Huang Jianjun Li Qingmiao Zhao Xiaolin Qu Tingli Zheng Qian |
| author_sort | Lu Bocheng |
| collection | DOAJ |
| description | Traditional Chinese orthopedics has a long history and excellent clinical effects in the treatment of osteoarthritis (OA). Bushen Tongluowan (BSTLW) is a representative prescription based on ''kidney governing bones'' theory in Traditional Chinese medicine. However, the molecular mechanism underlying its effect on articular chondrocytes remains unclear. In this study, we investigated the effect of BSTLW on primary chondrocytes and analyzed the gene expression of chondrocytes after drug treatment using RNA-Seq. The results showed that BSTLW significantly improved chondrocyte proliferation in a dose-dependent manner. This effect is achieved through the regulation of multiple genes, including the upregulation of proproliferative and apoptotic inhibitors and the downregulation of pro-apoptotic and proliferative inhibitors. At the same time, chondrocyte differentiation was inhibited, and chondrocyte proliferation remained active. This study fills the gap in the molecular effect of BSTLW on chondrocytes. |
| first_indexed | 2024-04-11T06:13:19Z |
| format | Article |
| id | doaj.art-b489742067874af581711530fffbf612 |
| institution | Directory Open Access Journal |
| issn | 2667-1425 |
| language | English |
| last_indexed | 2024-04-11T06:13:19Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Pharmacological Research - Modern Chinese Medicine |
| spelling | doaj.art-b489742067874af581711530fffbf6122022-12-22T04:41:09ZengElsevierPharmacological Research - Modern Chinese Medicine2667-14252022-12-015100164Bushen Tongluowan promotes chondrocyte proliferation through multi-gene regulationLu Bocheng0Zhou Zhenwei1Zhang Pengcheng2Huang Jianjun3Li Qingmiao4Zhao Xiaolin5Qu Tingli6Zheng Qian7Shanxi Traditional Chinese Medicine Hospital, Taiyuan, Shanxi, ChinaDepartment of Pharmacy, Changchun University of Traditional Chinese Medicine, Changchun, Ji Lin, ChinaDepartment of Pharmacy, Changchun University of Traditional Chinese Medicine, Changchun, Ji Lin, China; Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, Ji Lin, ChinaShanxi Traditional Chinese Medicine Hospital, Taiyuan, Shanxi, ChinaDepartment of Pharmacy, Shanxi Medical University, Taiyuan, Shanxi, ChinaDepartment of Pharmacy, Shanxi Medical University, Taiyuan, Shanxi, ChinaDepartment of Pharmacy, Shanxi Medical University, Taiyuan, Shanxi, ChinaDepartment of Pharmacy, Shanxi Medical University, Taiyuan, Shanxi, China; Corresponding author at: Department of Pharmacy, Shanxi Medical University, Taiyuan, 030000, Shanxi, China.Traditional Chinese orthopedics has a long history and excellent clinical effects in the treatment of osteoarthritis (OA). Bushen Tongluowan (BSTLW) is a representative prescription based on ''kidney governing bones'' theory in Traditional Chinese medicine. However, the molecular mechanism underlying its effect on articular chondrocytes remains unclear. In this study, we investigated the effect of BSTLW on primary chondrocytes and analyzed the gene expression of chondrocytes after drug treatment using RNA-Seq. The results showed that BSTLW significantly improved chondrocyte proliferation in a dose-dependent manner. This effect is achieved through the regulation of multiple genes, including the upregulation of proproliferative and apoptotic inhibitors and the downregulation of pro-apoptotic and proliferative inhibitors. At the same time, chondrocyte differentiation was inhibited, and chondrocyte proliferation remained active. This study fills the gap in the molecular effect of BSTLW on chondrocytes.http://www.sciencedirect.com/science/article/pii/S2667142522001245Bushen TongluowanOsteoarthritisChondrocytesTraditional Chinese MedicineClassic Chinese formulation |
| spellingShingle | Lu Bocheng Zhou Zhenwei Zhang Pengcheng Huang Jianjun Li Qingmiao Zhao Xiaolin Qu Tingli Zheng Qian Bushen Tongluowan promotes chondrocyte proliferation through multi-gene regulation Pharmacological Research - Modern Chinese Medicine Bushen Tongluowan Osteoarthritis Chondrocytes Traditional Chinese Medicine Classic Chinese formulation |
| title | Bushen Tongluowan promotes chondrocyte proliferation through multi-gene regulation |
| title_full | Bushen Tongluowan promotes chondrocyte proliferation through multi-gene regulation |
| title_fullStr | Bushen Tongluowan promotes chondrocyte proliferation through multi-gene regulation |
| title_full_unstemmed | Bushen Tongluowan promotes chondrocyte proliferation through multi-gene regulation |
| title_short | Bushen Tongluowan promotes chondrocyte proliferation through multi-gene regulation |
| title_sort | bushen tongluowan promotes chondrocyte proliferation through multi gene regulation |
| topic | Bushen Tongluowan Osteoarthritis Chondrocytes Traditional Chinese Medicine Classic Chinese formulation |
| url | http://www.sciencedirect.com/science/article/pii/S2667142522001245 |
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