Circulating IgA/IgG memory B cells against Mycobacterium tuberculosis dormancy-associated antigens Rv2659c and Rv3128c in active and latent tuberculosis
Objective: To elucidate the antigenic potential of dormancy-associated antigens Rv2659c and Rv3128c of Mycobacterium tuberculosis by examining the persistence of specific IgG and IgA memory B cells (MBCs) among patients with active tuberculosis (TB), household contacts with latent tuberculosis (LTBI...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | International Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971221005890 |
| _version_ | 1819137730566684672 |
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| author | Phyu Thwe Soe Jariya Hanthamrongwit Chutiphon Saelee Soe Paing Kyaw Prasong Khaenam Saradee Warit Nusara Satproedprai Surakameth Mahasirimongkol Hideki Yanai Patchanee Chootong Chaniya Leepiyasakulchai |
| author_facet | Phyu Thwe Soe Jariya Hanthamrongwit Chutiphon Saelee Soe Paing Kyaw Prasong Khaenam Saradee Warit Nusara Satproedprai Surakameth Mahasirimongkol Hideki Yanai Patchanee Chootong Chaniya Leepiyasakulchai |
| author_sort | Phyu Thwe Soe |
| collection | DOAJ |
| description | Objective: To elucidate the antigenic potential of dormancy-associated antigens Rv2659c and Rv3128c of Mycobacterium tuberculosis by examining the persistence of specific IgG and IgA memory B cells (MBCs) among patients with active tuberculosis (TB), household contacts with latent tuberculosis (LTBI), and an endemic healthy control group. Methods: Fresh peripheral blood mononuclear cells from the three study groups were used to enumerate the numbers of IgG and IgA MBCs specific to recombinant protein Rv2659c and Rv3128c by ELISpot assay. The composition of MBC subsets IgA+ and IgG + was analyzed by flow cytometry. Results: The number of IgA MBCs specific to antigen Rv2659c was significantly higher in the LTBI group than the TB group. In contrast, no significant difference was found in IgA or IgG MBCs against antigen Rv3128c. The number of IgA+ MBCs was significantly higher than that of IgG+ MBCs in the classical MBC subset of the LTBI group. Conclusion: The results indicated that the dormancy-associated antigen Rv2659c induced an IgA MBCs response in individuals with latent TB, and IgA+ classical MBCs formed a major portion of the MBCs subset. This new knowledge will be beneficial for the development of novel TB vaccines and their control of latent TB. |
| first_indexed | 2024-12-22T10:55:31Z |
| format | Article |
| id | doaj.art-b48995d318e5478ca236b57f47613ca4 |
| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-12-22T10:55:31Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-b48995d318e5478ca236b57f47613ca42022-12-21T18:28:38ZengElsevierInternational Journal of Infectious Diseases1201-97122021-09-011107582Circulating IgA/IgG memory B cells against Mycobacterium tuberculosis dormancy-associated antigens Rv2659c and Rv3128c in active and latent tuberculosisPhyu Thwe Soe0Jariya Hanthamrongwit1Chutiphon Saelee2Soe Paing Kyaw3Prasong Khaenam4Saradee Warit5Nusara Satproedprai6Surakameth Mahasirimongkol7Hideki Yanai8Patchanee Chootong9Chaniya Leepiyasakulchai10Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand; Department of Medical Laboratory Technology, University of Medical Technology, Mandalay, MyanmarDepartment of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, ThailandDepartment of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, ThailandClinical Pathology Laboratory, (1000) Bedded General Hospital, Nay Pyi Taw, MyanmarCenter of Standardization and Product Validation, Faculty of Medical Technology, Mahidol University, Bangkok, ThailandIndustrial Tuberculosis Team, IMMBRG, National Center for Genetic Engineering and Biotechnology (BIOTEC), NSTDA, Pathum Thani, ThailandGenomic Medicine and Innovation Support Division, Department of Medical Sciences, Ministry of Public Health, ThailandGenomic Medicine and Innovation Support Division, Department of Medical Sciences, Ministry of Public Health, ThailandDepartment of Clinical Laboratory, Fukujuji Hospital, Japan Anti-Tuberculosis Association (JATA), Tokyo, JapanDepartment of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, ThailandDepartment of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand; Corresponding author: Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand.Objective: To elucidate the antigenic potential of dormancy-associated antigens Rv2659c and Rv3128c of Mycobacterium tuberculosis by examining the persistence of specific IgG and IgA memory B cells (MBCs) among patients with active tuberculosis (TB), household contacts with latent tuberculosis (LTBI), and an endemic healthy control group. Methods: Fresh peripheral blood mononuclear cells from the three study groups were used to enumerate the numbers of IgG and IgA MBCs specific to recombinant protein Rv2659c and Rv3128c by ELISpot assay. The composition of MBC subsets IgA+ and IgG + was analyzed by flow cytometry. Results: The number of IgA MBCs specific to antigen Rv2659c was significantly higher in the LTBI group than the TB group. In contrast, no significant difference was found in IgA or IgG MBCs against antigen Rv3128c. The number of IgA+ MBCs was significantly higher than that of IgG+ MBCs in the classical MBC subset of the LTBI group. Conclusion: The results indicated that the dormancy-associated antigen Rv2659c induced an IgA MBCs response in individuals with latent TB, and IgA+ classical MBCs formed a major portion of the MBCs subset. This new knowledge will be beneficial for the development of novel TB vaccines and their control of latent TB.http://www.sciencedirect.com/science/article/pii/S1201971221005890Latent tuberculosis infectionRv2659cRv3128cMemory B cells |
| spellingShingle | Phyu Thwe Soe Jariya Hanthamrongwit Chutiphon Saelee Soe Paing Kyaw Prasong Khaenam Saradee Warit Nusara Satproedprai Surakameth Mahasirimongkol Hideki Yanai Patchanee Chootong Chaniya Leepiyasakulchai Circulating IgA/IgG memory B cells against Mycobacterium tuberculosis dormancy-associated antigens Rv2659c and Rv3128c in active and latent tuberculosis International Journal of Infectious Diseases Latent tuberculosis infection Rv2659c Rv3128c Memory B cells |
| title | Circulating IgA/IgG memory B cells against Mycobacterium tuberculosis dormancy-associated antigens Rv2659c and Rv3128c in active and latent tuberculosis |
| title_full | Circulating IgA/IgG memory B cells against Mycobacterium tuberculosis dormancy-associated antigens Rv2659c and Rv3128c in active and latent tuberculosis |
| title_fullStr | Circulating IgA/IgG memory B cells against Mycobacterium tuberculosis dormancy-associated antigens Rv2659c and Rv3128c in active and latent tuberculosis |
| title_full_unstemmed | Circulating IgA/IgG memory B cells against Mycobacterium tuberculosis dormancy-associated antigens Rv2659c and Rv3128c in active and latent tuberculosis |
| title_short | Circulating IgA/IgG memory B cells against Mycobacterium tuberculosis dormancy-associated antigens Rv2659c and Rv3128c in active and latent tuberculosis |
| title_sort | circulating iga igg memory b cells against mycobacterium tuberculosis dormancy associated antigens rv2659c and rv3128c in active and latent tuberculosis |
| topic | Latent tuberculosis infection Rv2659c Rv3128c Memory B cells |
| url | http://www.sciencedirect.com/science/article/pii/S1201971221005890 |
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