Effects of pH and Temperature on the Stability of Fumonisins in Maize Products
This paper is a study of the stability of fumonisins in dough based on maize flour prepared in a phosphate buffer with a pH of 3.5, 5.5 or 7.5 and baked at a temperature within the range of 100–250 °C. Buffers with various pH values were tested, since it is well-known that pH may significantly influ...
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MDPI AG
2017-03-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/9/3/88 |
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| author | Marcin Bryła Agnieszka Waśkiewicz Krystyna Szymczyk Renata Jędrzejczak |
| author_facet | Marcin Bryła Agnieszka Waśkiewicz Krystyna Szymczyk Renata Jędrzejczak |
| author_sort | Marcin Bryła |
| collection | DOAJ |
| description | This paper is a study of the stability of fumonisins in dough based on maize flour prepared in a phosphate buffer with a pH of 3.5, 5.5 or 7.5 and baked at a temperature within the range of 100–250 °C. Buffers with various pH values were tested, since it is well-known that pH may significantly influence interactions of fumonisins with other substances. A standard analytical procedure was used to determine the concentration of free fumonisins. Hydrolysis in an alkaline medium was then applied to reveal the hidden forms, while the total fumonisins concentations was determined in another measurement. The total concentration of fumonisins was statistically higher in pH = 3.5 and pH = 5.5 than the concentration of free fumonisins; no similar difference was found at pH = 7.5. The applied phosphate buffer pH 7.5 may enhance solubility of fumonisins, which would increase extraction efficiency of free analytes, thereby decreasing the difference between concentrations of total and free fumonisins. Hydrolysed B1 fumonisin (HFB1) and partially hydrolysed B1 fumonisin (isomers a and b: PHFB1a and PHFB1b, respectively) were the main investigated substances. For baking temperatures below 220 °C, fumonisins were slightly more stable for pH = 5.5 than for pH = 3.5 and pH = 7.5. In both of these latter cases, the concentration of partially hydrolysed fumonisins grew initially (up to 200 °C) with an increase in the baking temperature, and then dropped. Similar behaviour was observed for free HFB1, which may suggest the following fumonisin degradation mechanism: initially, the tricarballylic acid (TCA) groups are removed from the molecules, and next, the HFB1 molecules disintegrate. |
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| language | English |
| last_indexed | 2024-04-13T09:02:37Z |
| publishDate | 2017-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-b48b02de244e4a01a6a7cbd94f1db1532022-12-22T02:53:07ZengMDPI AGToxins2072-66512017-03-01938810.3390/toxins9030088toxins9030088Effects of pH and Temperature on the Stability of Fumonisins in Maize ProductsMarcin Bryła0Agnieszka Waśkiewicz1Krystyna Szymczyk2Renata Jędrzejczak3Department of Food Analysis, Prof. Waclaw Dabrowski Institute of Agricultural and Food Biotechnology, Rakowiecka 36, 02-532 Warsaw, PolandDepartment of Chemistry, Poznan University of Life Sciences, Wojska Polskiego 75, 60-625 Poznan, PolandDepartment of Food Analysis, Prof. Waclaw Dabrowski Institute of Agricultural and Food Biotechnology, Rakowiecka 36, 02-532 Warsaw, PolandDepartment of Food Analysis, Prof. Waclaw Dabrowski Institute of Agricultural and Food Biotechnology, Rakowiecka 36, 02-532 Warsaw, PolandThis paper is a study of the stability of fumonisins in dough based on maize flour prepared in a phosphate buffer with a pH of 3.5, 5.5 or 7.5 and baked at a temperature within the range of 100–250 °C. Buffers with various pH values were tested, since it is well-known that pH may significantly influence interactions of fumonisins with other substances. A standard analytical procedure was used to determine the concentration of free fumonisins. Hydrolysis in an alkaline medium was then applied to reveal the hidden forms, while the total fumonisins concentations was determined in another measurement. The total concentration of fumonisins was statistically higher in pH = 3.5 and pH = 5.5 than the concentration of free fumonisins; no similar difference was found at pH = 7.5. The applied phosphate buffer pH 7.5 may enhance solubility of fumonisins, which would increase extraction efficiency of free analytes, thereby decreasing the difference between concentrations of total and free fumonisins. Hydrolysed B1 fumonisin (HFB1) and partially hydrolysed B1 fumonisin (isomers a and b: PHFB1a and PHFB1b, respectively) were the main investigated substances. For baking temperatures below 220 °C, fumonisins were slightly more stable for pH = 5.5 than for pH = 3.5 and pH = 7.5. In both of these latter cases, the concentration of partially hydrolysed fumonisins grew initially (up to 200 °C) with an increase in the baking temperature, and then dropped. Similar behaviour was observed for free HFB1, which may suggest the following fumonisin degradation mechanism: initially, the tricarballylic acid (TCA) groups are removed from the molecules, and next, the HFB1 molecules disintegrate.http://www.mdpi.com/2072-6651/9/3/88fumonisinsconjugated fumonisinsLC-TOF-HRMSmaize productsstability of fumonisinspH and temperature conditions |
| spellingShingle | Marcin Bryła Agnieszka Waśkiewicz Krystyna Szymczyk Renata Jędrzejczak Effects of pH and Temperature on the Stability of Fumonisins in Maize Products Toxins fumonisins conjugated fumonisins LC-TOF-HRMS maize products stability of fumonisins pH and temperature conditions |
| title | Effects of pH and Temperature on the Stability of Fumonisins in Maize Products |
| title_full | Effects of pH and Temperature on the Stability of Fumonisins in Maize Products |
| title_fullStr | Effects of pH and Temperature on the Stability of Fumonisins in Maize Products |
| title_full_unstemmed | Effects of pH and Temperature on the Stability of Fumonisins in Maize Products |
| title_short | Effects of pH and Temperature on the Stability of Fumonisins in Maize Products |
| title_sort | effects of ph and temperature on the stability of fumonisins in maize products |
| topic | fumonisins conjugated fumonisins LC-TOF-HRMS maize products stability of fumonisins pH and temperature conditions |
| url | http://www.mdpi.com/2072-6651/9/3/88 |
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