Biogenic silver nanoparticles improve bioactive compounds in medicinal plant Juniperus procera in vitro
Bioactive compounds of medicinal plants present as natural ingredients provide health benefits beyond the basic nutritional value of these products. However, the availability of bioactive compounds in the current natural sources is limited. Hence, the induction of bioactive compound production from...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.962112/full |
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| author | Abdalrhaman M. Salih Fahad Al-Qurainy Salim Khan Mohammad Nadeem Mohamed Tarroum Hassan O. Shaikhaldein |
| author_facet | Abdalrhaman M. Salih Fahad Al-Qurainy Salim Khan Mohammad Nadeem Mohamed Tarroum Hassan O. Shaikhaldein |
| author_sort | Abdalrhaman M. Salih |
| collection | DOAJ |
| description | Bioactive compounds of medicinal plants present as natural ingredients provide health benefits beyond the basic nutritional value of these products. However, the availability of bioactive compounds in the current natural sources is limited. Hence, the induction of bioactive compound production from medicinal plants through nanoparticles (NPs) might play a vital role in industrially important medicinal compounds. Therefore, this study aimed to synthesize silver nanoparticles (AgNPs) biologically and to investigate their effect on phytochemical compound production from the callus of Juniperus procera. AgNPs were synthesized biologically using aqueous leaf extract of Phoenix dactylifera, which acted as a reducing and capping agent, and silver nitrate solution. The formation of AgNPs has been confirmed through different analytical techniques such as UV-Visible spectroscopy (UV), Fourier-transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and scanning electron microscope (SEM). The impact of different concentrations (0.0, 5, 20, and 50 mg/L) of AgNPs on enzymatic and non-enzymatic antioxidants of the callus of J. procera was investigated. The obtained results showed a significant effect of AgNPs on biomass accumulation and non-enzymatic antioxidants (phenol, tannin, and flavonoid content). Additionally, total protein content and superoxide dismutase (SOD) activity were increased in response to AgNPs. Furthermore, bioactive compounds like gallic acid, tannic acid, coumarin, hesperidin, rutin, quercetin, and ferruginol were chromatographically separated and quantified using high-performance liquid chromatography (HPLC) with reference standards. These compounds were increased significantly in response to AgNPs treatments. We concluded that AgNPs could be a promising elicitor for improving the production of phytochemical compounds in medicinal plants. This work can serve asa good model for improving the production of bioactive compounds from medicinal plants in vitro. This molecular investigation should be done to understand better the metabolic mechanism leading to bioactive compound production scaling. |
| first_indexed | 2024-04-12T18:19:01Z |
| format | Article |
| id | doaj.art-b48e3dcd17c441e9a1733d895367f017 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T18:19:01Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-b48e3dcd17c441e9a1733d895367f0172022-12-22T03:21:31ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.962112962112Biogenic silver nanoparticles improve bioactive compounds in medicinal plant Juniperus procera in vitroAbdalrhaman M. SalihFahad Al-QurainySalim KhanMohammad NadeemMohamed TarroumHassan O. ShaikhaldeinBioactive compounds of medicinal plants present as natural ingredients provide health benefits beyond the basic nutritional value of these products. However, the availability of bioactive compounds in the current natural sources is limited. Hence, the induction of bioactive compound production from medicinal plants through nanoparticles (NPs) might play a vital role in industrially important medicinal compounds. Therefore, this study aimed to synthesize silver nanoparticles (AgNPs) biologically and to investigate their effect on phytochemical compound production from the callus of Juniperus procera. AgNPs were synthesized biologically using aqueous leaf extract of Phoenix dactylifera, which acted as a reducing and capping agent, and silver nitrate solution. The formation of AgNPs has been confirmed through different analytical techniques such as UV-Visible spectroscopy (UV), Fourier-transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and scanning electron microscope (SEM). The impact of different concentrations (0.0, 5, 20, and 50 mg/L) of AgNPs on enzymatic and non-enzymatic antioxidants of the callus of J. procera was investigated. The obtained results showed a significant effect of AgNPs on biomass accumulation and non-enzymatic antioxidants (phenol, tannin, and flavonoid content). Additionally, total protein content and superoxide dismutase (SOD) activity were increased in response to AgNPs. Furthermore, bioactive compounds like gallic acid, tannic acid, coumarin, hesperidin, rutin, quercetin, and ferruginol were chromatographically separated and quantified using high-performance liquid chromatography (HPLC) with reference standards. These compounds were increased significantly in response to AgNPs treatments. We concluded that AgNPs could be a promising elicitor for improving the production of phytochemical compounds in medicinal plants. This work can serve asa good model for improving the production of bioactive compounds from medicinal plants in vitro. This molecular investigation should be done to understand better the metabolic mechanism leading to bioactive compound production scaling.https://www.frontiersin.org/articles/10.3389/fpls.2022.962112/fullbiosynthesisAgNPsbioactive compoundschromatographic analysismedicinal plantferruginol |
| spellingShingle | Abdalrhaman M. Salih Fahad Al-Qurainy Salim Khan Mohammad Nadeem Mohamed Tarroum Hassan O. Shaikhaldein Biogenic silver nanoparticles improve bioactive compounds in medicinal plant Juniperus procera in vitro Frontiers in Plant Science biosynthesis AgNPs bioactive compounds chromatographic analysis medicinal plant ferruginol |
| title | Biogenic silver nanoparticles improve bioactive compounds in medicinal plant Juniperus procera in vitro |
| title_full | Biogenic silver nanoparticles improve bioactive compounds in medicinal plant Juniperus procera in vitro |
| title_fullStr | Biogenic silver nanoparticles improve bioactive compounds in medicinal plant Juniperus procera in vitro |
| title_full_unstemmed | Biogenic silver nanoparticles improve bioactive compounds in medicinal plant Juniperus procera in vitro |
| title_short | Biogenic silver nanoparticles improve bioactive compounds in medicinal plant Juniperus procera in vitro |
| title_sort | biogenic silver nanoparticles improve bioactive compounds in medicinal plant juniperus procera in vitro |
| topic | biosynthesis AgNPs bioactive compounds chromatographic analysis medicinal plant ferruginol |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.962112/full |
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