Human Lymphocyte-Protective Effects of an Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae) Fruit Pulp
The current study aimed to evaluate, in vitro, the antioxidant capacity and the human lymphocyte-protective effect of the ethanolic extract from Detarium microcarpum fruit pulp against oxidative stress damage. Human lymphocytes were incubated with different concentrations of extract, followed by the...
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MDPI AG
2018-08-01
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| Series: | Antioxidants |
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| Online Access: | http://www.mdpi.com/2076-3921/7/8/104 |
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| author | Ablassé Rouamba Moussa Compaoré Maurice Ouédraogo Martin Kiendrebeogo |
| author_facet | Ablassé Rouamba Moussa Compaoré Maurice Ouédraogo Martin Kiendrebeogo |
| author_sort | Ablassé Rouamba |
| collection | DOAJ |
| description | The current study aimed to evaluate, in vitro, the antioxidant capacity and the human lymphocyte-protective effect of the ethanolic extract from Detarium microcarpum fruit pulp against oxidative stress damage. Human lymphocytes were incubated with different concentrations of extract, followed by the addition of hydrogen peroxide or tert-butyl hydroperoxide. Cell viability was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Furthermore, the antioxidant property of the extract was evaluated in vitro using hydrogen peroxide and nitric oxide radical-scavenging assays. Compared to the vehicle, the fruit pulp ethanol extract did not exhibit a cytotoxic effect on human lymphocytes. Furthermore, the cytotoxicity of hydrogen peroxide and tert-butyl hydroperoxide to human lymphocytes was significantly reduced by fruit extract pretreatment. The extract and ascorbic acid exhibited similar cytoprotective activity (p > 0.05). The fruit pulp extract showed more antioxidant activity than gallic acid in the hydrogen peroxide-scavenging model, while in the nitric oxide-quenching model, the fruit extract and gallic acid showed similar activity. The fruit pulp of D. microcarpum contains potent antioxidant and cell-protective compounds. The use of the fruit pulp of D. microcarpum as a food supplement could rescue cellular oxidative damage responsible for numerous pathologies. |
| first_indexed | 2024-03-12T08:14:12Z |
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| id | doaj.art-b490afcad70e471890737c5ec6135f4e |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T08:14:12Z |
| publishDate | 2018-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-b490afcad70e471890737c5ec6135f4e2023-09-02T19:02:21ZengMDPI AGAntioxidants2076-39212018-08-017810410.3390/antiox7080104antiox7080104Human Lymphocyte-Protective Effects of an Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae) Fruit PulpAblassé Rouamba0Moussa Compaoré1Maurice Ouédraogo2Martin Kiendrebeogo3Laboratory of Applied Biochemistry and Chemistry (LABIOCA), UFR-SVT, University Ouaga 1 Pr Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina FasoLaboratory of Applied Biochemistry and Chemistry (LABIOCA), UFR-SVT, University Ouaga 1 Pr Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina FasoLaboratory of Animal Physiology (LPA), UFR-SVT, University Ouaga 1 Pr Joseph+ KI-ZERBO, 09 BP 848, Ouagadougou 09, Burkina FasoLaboratory of Applied Biochemistry and Chemistry (LABIOCA), UFR-SVT, University Ouaga 1 Pr Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina FasoThe current study aimed to evaluate, in vitro, the antioxidant capacity and the human lymphocyte-protective effect of the ethanolic extract from Detarium microcarpum fruit pulp against oxidative stress damage. Human lymphocytes were incubated with different concentrations of extract, followed by the addition of hydrogen peroxide or tert-butyl hydroperoxide. Cell viability was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Furthermore, the antioxidant property of the extract was evaluated in vitro using hydrogen peroxide and nitric oxide radical-scavenging assays. Compared to the vehicle, the fruit pulp ethanol extract did not exhibit a cytotoxic effect on human lymphocytes. Furthermore, the cytotoxicity of hydrogen peroxide and tert-butyl hydroperoxide to human lymphocytes was significantly reduced by fruit extract pretreatment. The extract and ascorbic acid exhibited similar cytoprotective activity (p > 0.05). The fruit pulp extract showed more antioxidant activity than gallic acid in the hydrogen peroxide-scavenging model, while in the nitric oxide-quenching model, the fruit extract and gallic acid showed similar activity. The fruit pulp of D. microcarpum contains potent antioxidant and cell-protective compounds. The use of the fruit pulp of D. microcarpum as a food supplement could rescue cellular oxidative damage responsible for numerous pathologies.http://www.mdpi.com/2076-3921/7/8/104antioxidantcytoprotectivehydrogen peroxidetert-butyl hydroperoxide |
| spellingShingle | Ablassé Rouamba Moussa Compaoré Maurice Ouédraogo Martin Kiendrebeogo Human Lymphocyte-Protective Effects of an Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae) Fruit Pulp Antioxidants antioxidant cytoprotective hydrogen peroxide tert-butyl hydroperoxide |
| title | Human Lymphocyte-Protective Effects of an Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae) Fruit Pulp |
| title_full | Human Lymphocyte-Protective Effects of an Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae) Fruit Pulp |
| title_fullStr | Human Lymphocyte-Protective Effects of an Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae) Fruit Pulp |
| title_full_unstemmed | Human Lymphocyte-Protective Effects of an Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae) Fruit Pulp |
| title_short | Human Lymphocyte-Protective Effects of an Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae) Fruit Pulp |
| title_sort | human lymphocyte protective effects of an ethanol extract from detarium microcarpum guill and perr caesalpiniaceae fruit pulp |
| topic | antioxidant cytoprotective hydrogen peroxide tert-butyl hydroperoxide |
| url | http://www.mdpi.com/2076-3921/7/8/104 |
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