Defined Pig Microbiota with a Potential Protective Effect against Infection with <i>Salmonella</i> Typhimurium
A balanced microbiota is a main prerequisite for the host’s health. The aim of the present work was to develop defined pig microbiota (DPM) with the potential ability to protect piglets against infection with <i>Salmonella</i> Typhimurium, which causes enterocolitis. A total of 284 bacte...
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MDPI AG
2023-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/4/1007 |
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| author | Kristyna Horvathova Nikol Modrackova Igor Splichal Alla Splichalova Ahmad Amin Eugenio Ingribelli Jiri Killer Ivo Doskocil Radko Pechar Tereza Kodesova Eva Vlkova |
| author_facet | Kristyna Horvathova Nikol Modrackova Igor Splichal Alla Splichalova Ahmad Amin Eugenio Ingribelli Jiri Killer Ivo Doskocil Radko Pechar Tereza Kodesova Eva Vlkova |
| author_sort | Kristyna Horvathova |
| collection | DOAJ |
| description | A balanced microbiota is a main prerequisite for the host’s health. The aim of the present work was to develop defined pig microbiota (DPM) with the potential ability to protect piglets against infection with <i>Salmonella</i> Typhimurium, which causes enterocolitis. A total of 284 bacterial strains were isolated from the colon and fecal samples of wild and domestic pigs or piglets using selective and nonselective cultivation media. Isolates belonging to 47 species from 11 different genera were identified by MALDI-TOF mass spectrometry (MALDI-TOF MS). The bacterial strains for the DPM were selected for anti-<i>Salmonella</i> activity, ability to aggregate, adherence to epithelial cells, and to be bile and acid tolerant. The selected combination of 9 strains was identified by sequencing of the 16S rRNA gene as <i>Bacillus</i> sp., <i>Bifidobacterium animalis</i> subsp. <i>lactis</i>, <i>B. porcinum</i>, <i>Clostridium sporogenes</i>, <i>Lactobacillus amylovorus</i>, <i>L. paracasei</i> subsp. <i>tolerans</i>, <i>Limosilactobacillus reuteri</i> subsp. <i>suis</i>, and <i>Limosilactobacillus reuteri</i> (two strains) did not show mutual inhibition, and the mixture was stable under freezing for at least 6 months. Moreover, strains were classified as safe without pathogenic phenotype and resistance to antibiotics. Future experiments with <i>Salmonella</i>-infected piglets are needed to test the protective effect of the developed DPM. |
| first_indexed | 2024-03-11T04:43:51Z |
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| language | English |
| last_indexed | 2024-03-11T04:43:51Z |
| publishDate | 2023-04-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-b4940ecbe7f745c88c990753b4a8bb9d2023-11-17T20:33:40ZengMDPI AGMicroorganisms2076-26072023-04-01114100710.3390/microorganisms11041007Defined Pig Microbiota with a Potential Protective Effect against Infection with <i>Salmonella</i> TyphimuriumKristyna Horvathova0Nikol Modrackova1Igor Splichal2Alla Splichalova3Ahmad Amin4Eugenio Ingribelli5Jiri Killer6Ivo Doskocil7Radko Pechar8Tereza Kodesova9Eva Vlkova10Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 165 00 Prague, Czech RepublicDepartment of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 165 00 Prague, Czech RepublicLaboratory of Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, 549 22 Novy Hradek, Czech RepublicLaboratory of Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, 549 22 Novy Hradek, Czech RepublicDepartment of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 165 00 Prague, Czech RepublicDepartment of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 165 00 Prague, Czech RepublicDepartment of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 165 00 Prague, Czech RepublicDepartment of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 165 00 Prague, Czech RepublicDepartment of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 165 00 Prague, Czech RepublicDepartment of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 165 00 Prague, Czech RepublicDepartment of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 165 00 Prague, Czech RepublicA balanced microbiota is a main prerequisite for the host’s health. The aim of the present work was to develop defined pig microbiota (DPM) with the potential ability to protect piglets against infection with <i>Salmonella</i> Typhimurium, which causes enterocolitis. A total of 284 bacterial strains were isolated from the colon and fecal samples of wild and domestic pigs or piglets using selective and nonselective cultivation media. Isolates belonging to 47 species from 11 different genera were identified by MALDI-TOF mass spectrometry (MALDI-TOF MS). The bacterial strains for the DPM were selected for anti-<i>Salmonella</i> activity, ability to aggregate, adherence to epithelial cells, and to be bile and acid tolerant. The selected combination of 9 strains was identified by sequencing of the 16S rRNA gene as <i>Bacillus</i> sp., <i>Bifidobacterium animalis</i> subsp. <i>lactis</i>, <i>B. porcinum</i>, <i>Clostridium sporogenes</i>, <i>Lactobacillus amylovorus</i>, <i>L. paracasei</i> subsp. <i>tolerans</i>, <i>Limosilactobacillus reuteri</i> subsp. <i>suis</i>, and <i>Limosilactobacillus reuteri</i> (two strains) did not show mutual inhibition, and the mixture was stable under freezing for at least 6 months. Moreover, strains were classified as safe without pathogenic phenotype and resistance to antibiotics. Future experiments with <i>Salmonella</i>-infected piglets are needed to test the protective effect of the developed DPM.https://www.mdpi.com/2076-2607/11/4/1007pig intestinal bacteriabacterial consortiumprobiotic properties testingintestinal pathogensgnotobiotic piglets |
| spellingShingle | Kristyna Horvathova Nikol Modrackova Igor Splichal Alla Splichalova Ahmad Amin Eugenio Ingribelli Jiri Killer Ivo Doskocil Radko Pechar Tereza Kodesova Eva Vlkova Defined Pig Microbiota with a Potential Protective Effect against Infection with <i>Salmonella</i> Typhimurium Microorganisms pig intestinal bacteria bacterial consortium probiotic properties testing intestinal pathogens gnotobiotic piglets |
| title | Defined Pig Microbiota with a Potential Protective Effect against Infection with <i>Salmonella</i> Typhimurium |
| title_full | Defined Pig Microbiota with a Potential Protective Effect against Infection with <i>Salmonella</i> Typhimurium |
| title_fullStr | Defined Pig Microbiota with a Potential Protective Effect against Infection with <i>Salmonella</i> Typhimurium |
| title_full_unstemmed | Defined Pig Microbiota with a Potential Protective Effect against Infection with <i>Salmonella</i> Typhimurium |
| title_short | Defined Pig Microbiota with a Potential Protective Effect against Infection with <i>Salmonella</i> Typhimurium |
| title_sort | defined pig microbiota with a potential protective effect against infection with i salmonella i typhimurium |
| topic | pig intestinal bacteria bacterial consortium probiotic properties testing intestinal pathogens gnotobiotic piglets |
| url | https://www.mdpi.com/2076-2607/11/4/1007 |
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