Effect of biological amniotic membrane soaked in pirfenidone on scar formation after glaucoma filtration surgery in rabbit eyes
AIM: To investigate the application of biological amniotic membrane soaked in pirfenidone(PFD)and to evaluate its anti-scarring effect and toxic side effects on glaucoma model of rabbit eyes.METHODS: The right eyes of 72 healthy New Zealand white rabbits were randomly divided into 0.5%PFD+ biologica...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2024-02-01
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| Series: | Guoji Yanke Zazhi |
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| Online Access: | http://ies.ijo.cn/cn_publish/2024/2/202402003.pdf |
| _version_ | 1797352795313012736 |
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| author | Zhang Shuai Yao Yihua Zheng Yangjing Wu Ping Zhu Yihua |
| author_facet | Zhang Shuai Yao Yihua Zheng Yangjing Wu Ping Zhu Yihua |
| author_sort | Zhang Shuai |
| collection | DOAJ |
| description | AIM: To investigate the application of biological amniotic membrane soaked in pirfenidone(PFD)and to evaluate its anti-scarring effect and toxic side effects on glaucoma model of rabbit eyes.METHODS: The right eyes of 72 healthy New Zealand white rabbits were randomly divided into 0.5%PFD+ biological amniotic membrane group, biological amniotic membrane group, mitomycin C(MMC)group and blank control group after the glaucoma model was established by anterior chamber injection of compound carbomer solution, and 18 rabbits in each group underwent trabeculectomy, in which the 0.5% PFD+ biological amniotic membrane group was placed with 0.5% PFD solution-soaked biological amniotic membrane under the scleral flap, and the biological amniotic membrane group was placed with normal saline-soaked rehydrated biological amniotic membrane under the scleral flap. In the MMC group, a cotton pad soaked in MMC was placed under the scleral flap for 3 min and immediately rinsed with normal saline, while the blank control group received no implant after the scleral flap was made. The intraocular pressure(IOP), filtration blebs, toxic side effects and complications were evaluated, and the histopathological changes in the filtration area were observed by hematoxylin-eosin(HE), Masson staining and immunohistochemical staining.RESULTS: The mean IOP at 14, 21 and 28 d after trabeculectomy were 0.5%PFD+ biological amniotic membrane group<MMC group<biological amniotic membrane group<blank control group(all P<0.05). At 28 d after trabeculectomy, 0.5%PFD+ biological amniotic membrane group had the best effect of anti-inflammatory hyperplasia and inhibition of collagen formation, the highest survival rate of filtration blebs, and the inflammatory reaction was mild.CONCLUSION: Biological amniotic membrane soaked in pirfenidone has more obvious anti-scarring effect on glaucoma model, with less toxic side effects and good safety. |
| first_indexed | 2024-03-08T13:21:56Z |
| format | Article |
| id | doaj.art-b496e7297f3643b4a1f7f4864c02770c |
| institution | Directory Open Access Journal |
| issn | 1672-5123 |
| language | English |
| last_indexed | 2024-03-08T13:21:56Z |
| publishDate | 2024-02-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | Guoji Yanke Zazhi |
| spelling | doaj.art-b496e7297f3643b4a1f7f4864c02770c2024-01-18T03:35:38ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51232024-02-0124218919510.3980/j.issn.1672-5123.2024.2.03202402003Effect of biological amniotic membrane soaked in pirfenidone on scar formation after glaucoma filtration surgery in rabbit eyesZhang Shuai0Yao Yihua1Zheng Yangjing2Wu Ping3Zhu Yihua4Fuzhou Southeast Eye Hospital, Fuzhou 350008, Fujian Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, Fujian Province, ChinaFuzhou Southeast Eye Hospital, Fuzhou 350008, Fujian Province, ChinaDepartment of Pathology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, Fujian Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, Fujian Province, ChinaAIM: To investigate the application of biological amniotic membrane soaked in pirfenidone(PFD)and to evaluate its anti-scarring effect and toxic side effects on glaucoma model of rabbit eyes.METHODS: The right eyes of 72 healthy New Zealand white rabbits were randomly divided into 0.5%PFD+ biological amniotic membrane group, biological amniotic membrane group, mitomycin C(MMC)group and blank control group after the glaucoma model was established by anterior chamber injection of compound carbomer solution, and 18 rabbits in each group underwent trabeculectomy, in which the 0.5% PFD+ biological amniotic membrane group was placed with 0.5% PFD solution-soaked biological amniotic membrane under the scleral flap, and the biological amniotic membrane group was placed with normal saline-soaked rehydrated biological amniotic membrane under the scleral flap. In the MMC group, a cotton pad soaked in MMC was placed under the scleral flap for 3 min and immediately rinsed with normal saline, while the blank control group received no implant after the scleral flap was made. The intraocular pressure(IOP), filtration blebs, toxic side effects and complications were evaluated, and the histopathological changes in the filtration area were observed by hematoxylin-eosin(HE), Masson staining and immunohistochemical staining.RESULTS: The mean IOP at 14, 21 and 28 d after trabeculectomy were 0.5%PFD+ biological amniotic membrane group<MMC group<biological amniotic membrane group<blank control group(all P<0.05). At 28 d after trabeculectomy, 0.5%PFD+ biological amniotic membrane group had the best effect of anti-inflammatory hyperplasia and inhibition of collagen formation, the highest survival rate of filtration blebs, and the inflammatory reaction was mild.CONCLUSION: Biological amniotic membrane soaked in pirfenidone has more obvious anti-scarring effect on glaucoma model, with less toxic side effects and good safety.http://ies.ijo.cn/cn_publish/2024/2/202402003.pdfpirfenidonebiological amniotic membranecompound carbomerglaucoma modeltrabeculectomyfiltration blebsanti-scarring |
| spellingShingle | Zhang Shuai Yao Yihua Zheng Yangjing Wu Ping Zhu Yihua Effect of biological amniotic membrane soaked in pirfenidone on scar formation after glaucoma filtration surgery in rabbit eyes Guoji Yanke Zazhi pirfenidone biological amniotic membrane compound carbomer glaucoma model trabeculectomy filtration blebs anti-scarring |
| title | Effect of biological amniotic membrane soaked in pirfenidone on scar formation after glaucoma filtration surgery in rabbit eyes |
| title_full | Effect of biological amniotic membrane soaked in pirfenidone on scar formation after glaucoma filtration surgery in rabbit eyes |
| title_fullStr | Effect of biological amniotic membrane soaked in pirfenidone on scar formation after glaucoma filtration surgery in rabbit eyes |
| title_full_unstemmed | Effect of biological amniotic membrane soaked in pirfenidone on scar formation after glaucoma filtration surgery in rabbit eyes |
| title_short | Effect of biological amniotic membrane soaked in pirfenidone on scar formation after glaucoma filtration surgery in rabbit eyes |
| title_sort | effect of biological amniotic membrane soaked in pirfenidone on scar formation after glaucoma filtration surgery in rabbit eyes |
| topic | pirfenidone biological amniotic membrane compound carbomer glaucoma model trabeculectomy filtration blebs anti-scarring |
| url | http://ies.ijo.cn/cn_publish/2024/2/202402003.pdf |
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