Functional Analyses of Three Targeted DNA Antimicrobial Peptides Derived from Goats
With the increase in drug-resistant bacteria, new antibacterial drugs have emerged as a prominent area of research and development. Antimicrobial peptides (AMPs), as innate immune agents, have garnered significant attention due to their potent, rapid, and broad-spectrum antibacterial activity. This...
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/10/1453 |
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| author | Aili Wang Mengying Zhou Qian Chen Hui Jin Gaochi Xu Ruiyin Guo Jianmin Wang Ren Lai |
| author_facet | Aili Wang Mengying Zhou Qian Chen Hui Jin Gaochi Xu Ruiyin Guo Jianmin Wang Ren Lai |
| author_sort | Aili Wang |
| collection | DOAJ |
| description | With the increase in drug-resistant bacteria, new antibacterial drugs have emerged as a prominent area of research and development. Antimicrobial peptides (AMPs), as innate immune agents, have garnered significant attention due to their potent, rapid, and broad-spectrum antibacterial activity. This study focused on investigating the functionality of three AMPs (CATH 1, CATH 2, and MAP34-B) derived from goat submandibular glands. Among these AMPs, CATH 2 and MAP34-B exhibited direct antibacterial activity against both Gram-negative and Gram-positive bacteria, primarily targeting the bacterial membrane. Additionally, these two AMPs were found to have the potential to induce reactive oxygen species (ROS) production in bacterial cells and interact with bacterial genome DNA, which may play a crucial role in their mechanisms of action. Furthermore, both CATH 1 and CATH 2 demonstrated significant antioxidant activity, and all three AMPs exhibited potential anti-inflammatory activity. Importantly, the cytotoxic activity of these AMPs against mammalian cells was found to be weak, and their hemolytic activity was extremely low. Overall, the characteristics of these three AMPs found in goat submandibular glands offer new insights for the study of host protection from an immunological perspective. They hold promise as potential candidates for the development of novel antibacterial agents, particularly in the context of combating drug-resistant bacteria. |
| first_indexed | 2024-03-10T21:24:46Z |
| format | Article |
| id | doaj.art-b49832bbb31b4fbdb76d2da0076cd151 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T21:24:46Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-b49832bbb31b4fbdb76d2da0076cd1512023-11-19T15:49:33ZengMDPI AGBiomolecules2218-273X2023-09-011310145310.3390/biom13101453Functional Analyses of Three Targeted DNA Antimicrobial Peptides Derived from GoatsAili Wang0Mengying Zhou1Qian Chen2Hui Jin3Gaochi Xu4Ruiyin Guo5Jianmin Wang6Ren Lai7Center for Evolution and Conservation Biology, Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, ChinaSuzhou Guangji Hospital, The Affiliated Guangji Hospital of Soochow University, Suzhou 215137, ChinaCenter for Evolution and Conservation Biology, Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, ChinaCenter for Evolution and Conservation Biology, Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, ChinaCenter for Evolution and Conservation Biology, Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, ChinaCenter for Evolution and Conservation Biology, Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271000, ChinaCenter for Evolution and Conservation Biology, Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, ChinaWith the increase in drug-resistant bacteria, new antibacterial drugs have emerged as a prominent area of research and development. Antimicrobial peptides (AMPs), as innate immune agents, have garnered significant attention due to their potent, rapid, and broad-spectrum antibacterial activity. This study focused on investigating the functionality of three AMPs (CATH 1, CATH 2, and MAP34-B) derived from goat submandibular glands. Among these AMPs, CATH 2 and MAP34-B exhibited direct antibacterial activity against both Gram-negative and Gram-positive bacteria, primarily targeting the bacterial membrane. Additionally, these two AMPs were found to have the potential to induce reactive oxygen species (ROS) production in bacterial cells and interact with bacterial genome DNA, which may play a crucial role in their mechanisms of action. Furthermore, both CATH 1 and CATH 2 demonstrated significant antioxidant activity, and all three AMPs exhibited potential anti-inflammatory activity. Importantly, the cytotoxic activity of these AMPs against mammalian cells was found to be weak, and their hemolytic activity was extremely low. Overall, the characteristics of these three AMPs found in goat submandibular glands offer new insights for the study of host protection from an immunological perspective. They hold promise as potential candidates for the development of novel antibacterial agents, particularly in the context of combating drug-resistant bacteria.https://www.mdpi.com/2218-273X/13/10/1453goat submandibular glandsantimicrobial peptidesantimicrobialantioxidantanti-inflammatoryDNA-binding |
| spellingShingle | Aili Wang Mengying Zhou Qian Chen Hui Jin Gaochi Xu Ruiyin Guo Jianmin Wang Ren Lai Functional Analyses of Three Targeted DNA Antimicrobial Peptides Derived from Goats Biomolecules goat submandibular glands antimicrobial peptides antimicrobial antioxidant anti-inflammatory DNA-binding |
| title | Functional Analyses of Three Targeted DNA Antimicrobial Peptides Derived from Goats |
| title_full | Functional Analyses of Three Targeted DNA Antimicrobial Peptides Derived from Goats |
| title_fullStr | Functional Analyses of Three Targeted DNA Antimicrobial Peptides Derived from Goats |
| title_full_unstemmed | Functional Analyses of Three Targeted DNA Antimicrobial Peptides Derived from Goats |
| title_short | Functional Analyses of Three Targeted DNA Antimicrobial Peptides Derived from Goats |
| title_sort | functional analyses of three targeted dna antimicrobial peptides derived from goats |
| topic | goat submandibular glands antimicrobial peptides antimicrobial antioxidant anti-inflammatory DNA-binding |
| url | https://www.mdpi.com/2218-273X/13/10/1453 |
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