Carbon nanoparticles enhance potassium uptake via upregulating potassium channel expression and imitating biological ion channels in BY-2 cells
Abstract Background Carbon nanoparticles (CNPs) have been reported to boost plant growth, while the mechanism that CNPs enhanced potassium uptake for plant growth has not been reported so far. Results In this study, the function that CNPs promoted potassium uptake in BY-2 cells was established and t...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-01-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-020-0581-0 |
| _version_ | 1811182958037434368 |
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| author | Lijuan Chen Jinchu Yang Xiang Li Taibo Liang Cong Nie Fuwei Xie Kejian Liu Xiaojun Peng Jianping Xie |
| author_facet | Lijuan Chen Jinchu Yang Xiang Li Taibo Liang Cong Nie Fuwei Xie Kejian Liu Xiaojun Peng Jianping Xie |
| author_sort | Lijuan Chen |
| collection | DOAJ |
| description | Abstract Background Carbon nanoparticles (CNPs) have been reported to boost plant growth, while the mechanism that CNPs enhanced potassium uptake for plant growth has not been reported so far. Results In this study, the function that CNPs promoted potassium uptake in BY-2 cells was established and the potassium accumulated in cells had a significant correlation with the fresh biomass of BY-2 cells. The K+ accumulation in cells increased with the increasing concentration of CNPs. The K+ influx reached high level after treatment with CNPs and was significantly higher than that of the control group and the negative group treated with K+ channels blocker, tetraethylammonium chloride (TEA+). The K+ accumulation was not reduced in the presence of CNPs inhibitors. In the presence of potassium channel blocker TEA+ or CNPs inhibitors, the NKT1 gene expression was changed compared with the control group. The CNPs were found to preferentially transport K+ than other cations determined by rectification of ion current assay (RIC) in a conical nanocapillary. Conclusions These results indicated that CNPs upregulated potassium gene expression to enhance K+ accumulation in BY-2 cells. Moreover, it was speculated that the CNPs simulated protein of ion channels via bulk of carboxyl for K+ permeating. These findings will provide support for improving plant growth by carbon nanoparticles. |
| first_indexed | 2024-04-11T09:39:06Z |
| format | Article |
| id | doaj.art-b499bcf230fc492cbd244af949198394 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-11T09:39:06Z |
| publishDate | 2020-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-b499bcf230fc492cbd244af9491983942022-12-22T04:31:22ZengBMCJournal of Nanobiotechnology1477-31552020-01-0118111410.1186/s12951-020-0581-0Carbon nanoparticles enhance potassium uptake via upregulating potassium channel expression and imitating biological ion channels in BY-2 cellsLijuan Chen0Jinchu Yang1Xiang Li2Taibo Liang3Cong Nie4Fuwei Xie5Kejian Liu6Xiaojun Peng7Jianping Xie8Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of SciencesTechnology Center, China Tobacco Henan Industrial Co. LtdZhengzhou Tobacco Research Institute of CNTCZhengzhou Tobacco Research Institute of CNTCZhengzhou Tobacco Research Institute of CNTCZhengzhou Tobacco Research Institute of CNTCZhengzhou Tobacco Research Institute of CNTCState Key Laboratory of Fine Chemicals, Dalian University of TechnologyZhengzhou Tobacco Research Institute of CNTCAbstract Background Carbon nanoparticles (CNPs) have been reported to boost plant growth, while the mechanism that CNPs enhanced potassium uptake for plant growth has not been reported so far. Results In this study, the function that CNPs promoted potassium uptake in BY-2 cells was established and the potassium accumulated in cells had a significant correlation with the fresh biomass of BY-2 cells. The K+ accumulation in cells increased with the increasing concentration of CNPs. The K+ influx reached high level after treatment with CNPs and was significantly higher than that of the control group and the negative group treated with K+ channels blocker, tetraethylammonium chloride (TEA+). The K+ accumulation was not reduced in the presence of CNPs inhibitors. In the presence of potassium channel blocker TEA+ or CNPs inhibitors, the NKT1 gene expression was changed compared with the control group. The CNPs were found to preferentially transport K+ than other cations determined by rectification of ion current assay (RIC) in a conical nanocapillary. Conclusions These results indicated that CNPs upregulated potassium gene expression to enhance K+ accumulation in BY-2 cells. Moreover, it was speculated that the CNPs simulated protein of ion channels via bulk of carboxyl for K+ permeating. These findings will provide support for improving plant growth by carbon nanoparticles.https://doi.org/10.1186/s12951-020-0581-0Carbon nanoparticlesPotassium uptakeGene expressionIon channels |
| spellingShingle | Lijuan Chen Jinchu Yang Xiang Li Taibo Liang Cong Nie Fuwei Xie Kejian Liu Xiaojun Peng Jianping Xie Carbon nanoparticles enhance potassium uptake via upregulating potassium channel expression and imitating biological ion channels in BY-2 cells Journal of Nanobiotechnology Carbon nanoparticles Potassium uptake Gene expression Ion channels |
| title | Carbon nanoparticles enhance potassium uptake via upregulating potassium channel expression and imitating biological ion channels in BY-2 cells |
| title_full | Carbon nanoparticles enhance potassium uptake via upregulating potassium channel expression and imitating biological ion channels in BY-2 cells |
| title_fullStr | Carbon nanoparticles enhance potassium uptake via upregulating potassium channel expression and imitating biological ion channels in BY-2 cells |
| title_full_unstemmed | Carbon nanoparticles enhance potassium uptake via upregulating potassium channel expression and imitating biological ion channels in BY-2 cells |
| title_short | Carbon nanoparticles enhance potassium uptake via upregulating potassium channel expression and imitating biological ion channels in BY-2 cells |
| title_sort | carbon nanoparticles enhance potassium uptake via upregulating potassium channel expression and imitating biological ion channels in by 2 cells |
| topic | Carbon nanoparticles Potassium uptake Gene expression Ion channels |
| url | https://doi.org/10.1186/s12951-020-0581-0 |
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