mRNA and lncRNA Expression Profiling of Radiation-Induced Gastric Injury Reveals Potential Radiation-Responsive Transcription Factors
Radiation-induced gastric injury is a serious concern that may limit the duration and the delivered dose of radiation. However, the genome-wide molecular changes in stomach upon ionizing radiation have not been reported. In this study, mouse stomach was irradiated with 6 or 12 Gy X-ray irradiation a...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2019-11-01
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| Series: | Dose-Response |
| Online Access: | https://doi.org/10.1177/1559325819886766 |
| _version_ | 1818958329724010496 |
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| author | Guangxia Chen Yang Feng Zhiqiang Sun Yiying Gao Chuannan Wu Haihan Zhang Jinming Cao Zhuo Chen Jianping Cao Yaqun Zhu Shuyu Zhang |
| author_facet | Guangxia Chen Yang Feng Zhiqiang Sun Yiying Gao Chuannan Wu Haihan Zhang Jinming Cao Zhuo Chen Jianping Cao Yaqun Zhu Shuyu Zhang |
| author_sort | Guangxia Chen |
| collection | DOAJ |
| description | Radiation-induced gastric injury is a serious concern that may limit the duration and the delivered dose of radiation. However, the genome-wide molecular changes in stomach upon ionizing radiation have not been reported. In this study, mouse stomach was irradiated with 6 or 12 Gy X-ray irradiation and we found that radiation resulted in the atrophy of gastric mucosa and abnormal morphology of chief and parietal cells. Radiation-induced gastric injury was accompanied by an increase in the serum levels of pepsinogen A and pepsinogen C but not gastrin-17. The expression profiles of messenger RNA (mRNA) and long noncoding RNA (lncRNA) in normal and irradiated gastric tissues were measured by microarray analysis. Results revealed 17 upregulated and 10 downregulated mRNAs were consistent in 6 and 12 Gy irradiated gastric tissues, including D site-binding protein ( Dbp ) and fibrinogen-like protein 1 ( Fgl1 ). Thirteen upregulated and 96 downregulated lncRNAs were commonly changed in 6 and 12 Gy irradiated gastric tissues. The dysregulated mRNAs were implicated in multiple pathways and showed coexpression with lncRNAs. To identify motifs for transcription factors and coactivators in the proximal promoter regions of the dysregulated RNAs, the bioinformatic tool Biopython was used. A variety of common motifs that are associated with transcription factors were identified, including ZNF263, LMX1B, and Dlx1. Our findings illustrate the molecular changes during radiation-induced gastric injury and the potential transcription factors driving this alteration. |
| first_indexed | 2024-12-20T11:24:01Z |
| format | Article |
| id | doaj.art-b49ea3dae5af4bf78276e75886096d66 |
| institution | Directory Open Access Journal |
| issn | 1559-3258 |
| language | English |
| last_indexed | 2024-12-20T11:24:01Z |
| publishDate | 2019-11-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Dose-Response |
| spelling | doaj.art-b49ea3dae5af4bf78276e75886096d662022-12-21T19:42:26ZengSAGE PublishingDose-Response1559-32582019-11-011710.1177/1559325819886766mRNA and lncRNA Expression Profiling of Radiation-Induced Gastric Injury Reveals Potential Radiation-Responsive Transcription FactorsGuangxia Chen0Yang Feng1Zhiqiang Sun2Yiying Gao3Chuannan Wu4Haihan Zhang5Jinming Cao6Zhuo Chen7Jianping Cao8Yaqun Zhu9Shuyu Zhang10 Department of Gastroenterology, First People’s Hospital of Xuzhou, Xuzhou Municipal Hospital Affiliated to Xuzhou Medical University, Xuzhou, China State Key Lab of Radiation Medicine and Radioprotection, Soochow University, Suzhou, China State Key Lab of Radiation Medicine and Radioprotection, Soochow University, Suzhou, China State Key Lab of Radiation Medicine and Radioprotection, Soochow University, Suzhou, China Department of Gastroenterology, First People’s Hospital of Xuzhou, Xuzhou Municipal Hospital Affiliated to Xuzhou Medical University, Xuzhou, China Department of Gastroenterology, First People’s Hospital of Xuzhou, Xuzhou Municipal Hospital Affiliated to Xuzhou Medical University, Xuzhou, China State Key Lab of Radiation Medicine and Radioprotection, Soochow University, Suzhou, China Department of Gastroenterology, First People’s Hospital of Xuzhou, Xuzhou Municipal Hospital Affiliated to Xuzhou Medical University, Xuzhou, China State Key Lab of Radiation Medicine and Radioprotection, Soochow University, Suzhou, China Department of Radiotherapy & Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, China Second Affiliated Hospital of Chengdu Medical College (China National Nuclear Corporation 416 Hospital), Chengdu, ChinaRadiation-induced gastric injury is a serious concern that may limit the duration and the delivered dose of radiation. However, the genome-wide molecular changes in stomach upon ionizing radiation have not been reported. In this study, mouse stomach was irradiated with 6 or 12 Gy X-ray irradiation and we found that radiation resulted in the atrophy of gastric mucosa and abnormal morphology of chief and parietal cells. Radiation-induced gastric injury was accompanied by an increase in the serum levels of pepsinogen A and pepsinogen C but not gastrin-17. The expression profiles of messenger RNA (mRNA) and long noncoding RNA (lncRNA) in normal and irradiated gastric tissues were measured by microarray analysis. Results revealed 17 upregulated and 10 downregulated mRNAs were consistent in 6 and 12 Gy irradiated gastric tissues, including D site-binding protein ( Dbp ) and fibrinogen-like protein 1 ( Fgl1 ). Thirteen upregulated and 96 downregulated lncRNAs were commonly changed in 6 and 12 Gy irradiated gastric tissues. The dysregulated mRNAs were implicated in multiple pathways and showed coexpression with lncRNAs. To identify motifs for transcription factors and coactivators in the proximal promoter regions of the dysregulated RNAs, the bioinformatic tool Biopython was used. A variety of common motifs that are associated with transcription factors were identified, including ZNF263, LMX1B, and Dlx1. Our findings illustrate the molecular changes during radiation-induced gastric injury and the potential transcription factors driving this alteration.https://doi.org/10.1177/1559325819886766 |
| spellingShingle | Guangxia Chen Yang Feng Zhiqiang Sun Yiying Gao Chuannan Wu Haihan Zhang Jinming Cao Zhuo Chen Jianping Cao Yaqun Zhu Shuyu Zhang mRNA and lncRNA Expression Profiling of Radiation-Induced Gastric Injury Reveals Potential Radiation-Responsive Transcription Factors Dose-Response |
| title | mRNA and lncRNA Expression Profiling of Radiation-Induced Gastric Injury Reveals Potential Radiation-Responsive Transcription Factors |
| title_full | mRNA and lncRNA Expression Profiling of Radiation-Induced Gastric Injury Reveals Potential Radiation-Responsive Transcription Factors |
| title_fullStr | mRNA and lncRNA Expression Profiling of Radiation-Induced Gastric Injury Reveals Potential Radiation-Responsive Transcription Factors |
| title_full_unstemmed | mRNA and lncRNA Expression Profiling of Radiation-Induced Gastric Injury Reveals Potential Radiation-Responsive Transcription Factors |
| title_short | mRNA and lncRNA Expression Profiling of Radiation-Induced Gastric Injury Reveals Potential Radiation-Responsive Transcription Factors |
| title_sort | mrna and lncrna expression profiling of radiation induced gastric injury reveals potential radiation responsive transcription factors |
| url | https://doi.org/10.1177/1559325819886766 |
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