A novel fluorescent sensor based on aptamer recognition and DNA walker amplification strategy and its determination of 17β-estradiol
17β-Estradiol (E2) is a common environmental endocrine and excessive estrogen will disturb the endocrine balance of human body and lead to diseases because of its accumulation in human body. The main aim of this study is to determine the trace amount of E2 in various foods. Based on aptamer specific...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187853522300802X |
| _version_ | 1797615945958555648 |
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| author | Yajun Zhang Licong Jia Wei Wang Meng Jiang Hongying Zhang LingMei Niu |
| author_facet | Yajun Zhang Licong Jia Wei Wang Meng Jiang Hongying Zhang LingMei Niu |
| author_sort | Yajun Zhang |
| collection | DOAJ |
| description | 17β-Estradiol (E2) is a common environmental endocrine and excessive estrogen will disturb the endocrine balance of human body and lead to diseases because of its accumulation in human body. The main aim of this study is to determine the trace amount of E2 in various foods. Based on aptamer specific recognition, DNA walker molecular machine and Exonuclease III-assisted dual cycle signal amplification technology, a fluorescent aptamer sensor was constructed and applied to the detection of 17β-estradiol. Atomic force microscopy (AFM) and polyacrylamide gel electrophoresis (PAGE) were used to verify the feasibility of DNA walker participating in the reaction. Under the optimal conditions, 17β-estradiol concentration showed a good linear relationship with fluorescence in the range of 5.0 × 10−10–1.8 × 10−8 M, and the detection limit was 5.6 × 10−11 M. The fluorescent aptamer sensor established in this study has good selectivity, simple operation and good specificity, and the results of its application in the determination of milk samples are satisfactory. Research findings revealed that the E2 concentration in water and milk is less than the safe limits, indicating the foods in the market are safe and can be consumed. Though the foods are safe in present content, the health risk still exists because of continuous intake of trace E2. |
| first_indexed | 2024-03-11T07:34:11Z |
| format | Article |
| id | doaj.art-b49f207fc1ca40a695c8d41d3a28d703 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-03-11T07:34:11Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-b49f207fc1ca40a695c8d41d3a28d7032023-11-17T05:26:04ZengElsevierArabian Journal of Chemistry1878-53522023-12-011612105340A novel fluorescent sensor based on aptamer recognition and DNA walker amplification strategy and its determination of 17β-estradiolYajun Zhang0Licong Jia1Wei Wang2Meng Jiang3Hongying Zhang4LingMei Niu5School of Public Health, Hebei Medical University, Shijiazhuang 050017, PR ChinaSchool of Public Health, Hebei Medical University, Shijiazhuang 050017, PR China; Hebei Key Laboratory of Environment and Human Health, Shijiazhuang 050017, PR ChinaSchool of Public Health, Hebei Medical University, Shijiazhuang 050017, PR China; Hebei Key Laboratory of Environment and Human Health, Shijiazhuang 050017, PR ChinaSchool of Public Health, Hebei Medical University, Shijiazhuang 050017, PR ChinaOutpatient Department of Hebei Medical University, Shijiazhuang 050017, PR ChinaSchool of Public Health, Hebei Medical University, Shijiazhuang 050017, PR China; Hebei Key Laboratory of Environment and Human Health, Shijiazhuang 050017, PR China; Hebei Key Laboratory of Forensic Medicine, Hebei Province, Shijiazhuang 050017, PR China; Corresponding author at: School of Public Health, Hebei Medical University, Shijiazhuang 050017, PR China.17β-Estradiol (E2) is a common environmental endocrine and excessive estrogen will disturb the endocrine balance of human body and lead to diseases because of its accumulation in human body. The main aim of this study is to determine the trace amount of E2 in various foods. Based on aptamer specific recognition, DNA walker molecular machine and Exonuclease III-assisted dual cycle signal amplification technology, a fluorescent aptamer sensor was constructed and applied to the detection of 17β-estradiol. Atomic force microscopy (AFM) and polyacrylamide gel electrophoresis (PAGE) were used to verify the feasibility of DNA walker participating in the reaction. Under the optimal conditions, 17β-estradiol concentration showed a good linear relationship with fluorescence in the range of 5.0 × 10−10–1.8 × 10−8 M, and the detection limit was 5.6 × 10−11 M. The fluorescent aptamer sensor established in this study has good selectivity, simple operation and good specificity, and the results of its application in the determination of milk samples are satisfactory. Research findings revealed that the E2 concentration in water and milk is less than the safe limits, indicating the foods in the market are safe and can be consumed. Though the foods are safe in present content, the health risk still exists because of continuous intake of trace E2.http://www.sciencedirect.com/science/article/pii/S187853522300802XAptamerFluorescence sensorDNA walkerExonuclease III17β-estradiol |
| spellingShingle | Yajun Zhang Licong Jia Wei Wang Meng Jiang Hongying Zhang LingMei Niu A novel fluorescent sensor based on aptamer recognition and DNA walker amplification strategy and its determination of 17β-estradiol Arabian Journal of Chemistry Aptamer Fluorescence sensor DNA walker Exonuclease III 17β-estradiol |
| title | A novel fluorescent sensor based on aptamer recognition and DNA walker amplification strategy and its determination of 17β-estradiol |
| title_full | A novel fluorescent sensor based on aptamer recognition and DNA walker amplification strategy and its determination of 17β-estradiol |
| title_fullStr | A novel fluorescent sensor based on aptamer recognition and DNA walker amplification strategy and its determination of 17β-estradiol |
| title_full_unstemmed | A novel fluorescent sensor based on aptamer recognition and DNA walker amplification strategy and its determination of 17β-estradiol |
| title_short | A novel fluorescent sensor based on aptamer recognition and DNA walker amplification strategy and its determination of 17β-estradiol |
| title_sort | novel fluorescent sensor based on aptamer recognition and dna walker amplification strategy and its determination of 17β estradiol |
| topic | Aptamer Fluorescence sensor DNA walker Exonuclease III 17β-estradiol |
| url | http://www.sciencedirect.com/science/article/pii/S187853522300802X |
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