Evaluation of banana cultivars and the pathogenesis-related class 3 and 10 proteins in defense against Ralstonia syzygii subsp. celebesensis, the causal agent of banana blood disease
Banana blood disease (BBD), caused by Ralstonia syzygii subsp. celebesensis ( Rsc), is a major threat to banana production in Southeast Asia. This study aimed to assess the resistance of cultivated and wild banana accessions to Rsc and investigate the expression of pathogenesis- related (PR) protein...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Polish Academy of Sciences
2023-09-01
|
| Series: | Journal of Plant Protection Research |
| Subjects: | |
| Online Access: | https://journals.pan.pl/Content/128317/PDF/OA_05_JPPR_63_3_1645_Nitayaros.pdf |
| _version_ | 1797681320032206848 |
|---|---|
| author | Jariya Nitayaros Thanwanit Thanyasiriwat Aphidech Sangdee Ladawan Rattanapolsan Ratri Boonruangrod Praphat Kawicha |
| author_facet | Jariya Nitayaros Thanwanit Thanyasiriwat Aphidech Sangdee Ladawan Rattanapolsan Ratri Boonruangrod Praphat Kawicha |
| author_sort | Jariya Nitayaros |
| collection | DOAJ |
| description | Banana blood disease (BBD), caused by Ralstonia syzygii subsp. celebesensis ( Rsc), is a major threat to banana production in Southeast Asia. This study aimed to assess the resistance of cultivated and wild banana accessions to Rsc and investigate the expression of pathogenesis- related (PR) protein genes, namely PR3 and PR10, in disease-resistant bananas. Bacterial isolates were isolated from infected bananas in Yala Province, Thailand, and their pathogenicity and phylotype were confirmed, along with Rsc-specific PCR. Rsc-resistance banana screening was conducted on 16 banana accessions, including cultivated and wild types, using representative Rsc isolates. ‘Khai Kasetsart 2’ exhibited resistance (R), followed by ‘Raksa’ with moderate resistance (MR). The expression of PR3 and PR10 genes was analyzed in the resistant ‘Khai Kasetsart 2’ and susceptible ‘Hin’ bananas, revealing distinct expression patterns. PR3 showed rapid upregulation on day 1 after inoculation (DAI), while PR10 exhibited sustained upregulation from 1 to 7 DAI in the resistant cultivar. These findings indicate the involvement of PR proteins in the defense response against Rsc and hold promise for future breeding and disease management strategies in bananas. |
| first_indexed | 2024-03-11T23:43:13Z |
| format | Article |
| id | doaj.art-b4a004d650ca43dc844613b7f5c568b2 |
| institution | Directory Open Access Journal |
| issn | 1427-4345 1899-007X |
| language | English |
| last_indexed | 2024-03-11T23:43:13Z |
| publishDate | 2023-09-01 |
| publisher | Polish Academy of Sciences |
| record_format | Article |
| series | Journal of Plant Protection Research |
| spelling | doaj.art-b4a004d650ca43dc844613b7f5c568b22023-09-19T15:19:53ZengPolish Academy of SciencesJournal of Plant Protection Research1427-43451899-007X2023-09-01vol. 63No 3https://doi.org/10.24425/jppr.2023.146873Evaluation of banana cultivars and the pathogenesis-related class 3 and 10 proteins in defense against Ralstonia syzygii subsp. celebesensis, the causal agent of banana blood diseaseJariya Nitayaros0Thanwanit Thanyasiriwat1Aphidech Sangdee2Ladawan Rattanapolsan3Ratri Boonruangrod4Praphat Kawicha5Plant Genome and Disease Research Unit, Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, ThailandPlant Genome and Disease Research Unit, Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, ThailandDepartment of Biology, Mahasarakham University, Faculty of Science, Maha Sarakham, ThailandPlant Genome and Disease Research Unit, Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, ThailandDepartment of Horticulture, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University Kamphaeng Saen Campus, Nakhon Pathom, ThailandPlant Genome and Disease Research Unit, Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, ThailandBanana blood disease (BBD), caused by Ralstonia syzygii subsp. celebesensis ( Rsc), is a major threat to banana production in Southeast Asia. This study aimed to assess the resistance of cultivated and wild banana accessions to Rsc and investigate the expression of pathogenesis- related (PR) protein genes, namely PR3 and PR10, in disease-resistant bananas. Bacterial isolates were isolated from infected bananas in Yala Province, Thailand, and their pathogenicity and phylotype were confirmed, along with Rsc-specific PCR. Rsc-resistance banana screening was conducted on 16 banana accessions, including cultivated and wild types, using representative Rsc isolates. ‘Khai Kasetsart 2’ exhibited resistance (R), followed by ‘Raksa’ with moderate resistance (MR). The expression of PR3 and PR10 genes was analyzed in the resistant ‘Khai Kasetsart 2’ and susceptible ‘Hin’ bananas, revealing distinct expression patterns. PR3 showed rapid upregulation on day 1 after inoculation (DAI), while PR10 exhibited sustained upregulation from 1 to 7 DAI in the resistant cultivar. These findings indicate the involvement of PR proteins in the defense response against Rsc and hold promise for future breeding and disease management strategies in bananas.https://journals.pan.pl/Content/128317/PDF/OA_05_JPPR_63_3_1645_Nitayaros.pdfbananablood diseasegene expressionpr generalstoniaresistance |
| spellingShingle | Jariya Nitayaros Thanwanit Thanyasiriwat Aphidech Sangdee Ladawan Rattanapolsan Ratri Boonruangrod Praphat Kawicha Evaluation of banana cultivars and the pathogenesis-related class 3 and 10 proteins in defense against Ralstonia syzygii subsp. celebesensis, the causal agent of banana blood disease Journal of Plant Protection Research banana blood disease gene expression pr gene ralstonia resistance |
| title | Evaluation of banana cultivars and the pathogenesis-related class 3 and 10 proteins in defense against Ralstonia syzygii subsp. celebesensis, the causal agent of banana blood disease |
| title_full | Evaluation of banana cultivars and the pathogenesis-related class 3 and 10 proteins in defense against Ralstonia syzygii subsp. celebesensis, the causal agent of banana blood disease |
| title_fullStr | Evaluation of banana cultivars and the pathogenesis-related class 3 and 10 proteins in defense against Ralstonia syzygii subsp. celebesensis, the causal agent of banana blood disease |
| title_full_unstemmed | Evaluation of banana cultivars and the pathogenesis-related class 3 and 10 proteins in defense against Ralstonia syzygii subsp. celebesensis, the causal agent of banana blood disease |
| title_short | Evaluation of banana cultivars and the pathogenesis-related class 3 and 10 proteins in defense against Ralstonia syzygii subsp. celebesensis, the causal agent of banana blood disease |
| title_sort | evaluation of banana cultivars and the pathogenesis related class 3 and 10 proteins in defense against ralstonia syzygii subsp celebesensis the causal agent of banana blood disease |
| topic | banana blood disease gene expression pr gene ralstonia resistance |
| url | https://journals.pan.pl/Content/128317/PDF/OA_05_JPPR_63_3_1645_Nitayaros.pdf |
| work_keys_str_mv | AT jariyanitayaros evaluationofbananacultivarsandthepathogenesisrelatedclass3and10proteinsindefenseagainstralstoniasyzygiisubspcelebesensisthecausalagentofbananablooddisease AT thanwanitthanyasiriwat evaluationofbananacultivarsandthepathogenesisrelatedclass3and10proteinsindefenseagainstralstoniasyzygiisubspcelebesensisthecausalagentofbananablooddisease AT aphidechsangdee evaluationofbananacultivarsandthepathogenesisrelatedclass3and10proteinsindefenseagainstralstoniasyzygiisubspcelebesensisthecausalagentofbananablooddisease AT ladawanrattanapolsan evaluationofbananacultivarsandthepathogenesisrelatedclass3and10proteinsindefenseagainstralstoniasyzygiisubspcelebesensisthecausalagentofbananablooddisease AT ratriboonruangrod evaluationofbananacultivarsandthepathogenesisrelatedclass3and10proteinsindefenseagainstralstoniasyzygiisubspcelebesensisthecausalagentofbananablooddisease AT praphatkawicha evaluationofbananacultivarsandthepathogenesisrelatedclass3and10proteinsindefenseagainstralstoniasyzygiisubspcelebesensisthecausalagentofbananablooddisease |