Blood Flow Restriction Exercise Attenuates the Exercise-Induced Endothelial Progenitor Cell Response in Healthy, Young Men
Endothelial progenitor cells (EPCs) are a vasculogenic subset of progenitors, which play a key role in maintenance of endothelial integrity. These cells are exercise-responsive, and thus exercise may play a key role in vascular repair and maintenance via mobilization of such cells. Blood flow restri...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-04-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2019.00447/full |
| _version_ | 1819053318794641408 |
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| author | Ryan Montgomery Allan Paterson Chris Williamson Geraint Florida-James Mark Daniel Ross |
| author_facet | Ryan Montgomery Allan Paterson Chris Williamson Geraint Florida-James Mark Daniel Ross |
| author_sort | Ryan Montgomery |
| collection | DOAJ |
| description | Endothelial progenitor cells (EPCs) are a vasculogenic subset of progenitors, which play a key role in maintenance of endothelial integrity. These cells are exercise-responsive, and thus exercise may play a key role in vascular repair and maintenance via mobilization of such cells. Blood flow restriction exercise, due to the augmentation of local tissue hypoxia, may promote exercise-induced EPC mobilization. Nine, healthy, young (18–30 years) males participated in the study. Participants undertook 2 trials of single leg knee extensor (KE) exercise, at 60% of thigh occlusion pressure (4 sets at 30% maximal torque) (blood flow restriction; BFR) or non- blood flow restriction (non-BFR), in a fasted state. Blood was taken prior, immediately after, and 30 min after exercise. Blood was used for the quantification of hematopoietic progenitor cells (HPCs: CD34+CD45dim), EPCs (CD34+VEGFR2+/CD34+CD45dimVEGFR2+) by flow cytometry. Our results show that unilateral KE exercise did not affect circulating HPC levels (p = 0.856), but did result in increases in both CD34+VEGFR2+ and CD34+CD45dimVEGFR2+ EPCs, but only in the non-BFR trial (CD34+VEGFR2+: 269 ± 42 cells mL-1 to 573 ± 90 cells mL-1, pre- to immediately post-exercise, p = 0.008; CD34+CD45dimVEGFR2+: 129 ± 21 cells mL-1 to 313 ± 103 cells mL-1, pre- to 30 min post-exercise, p = 0.010). In conclusion, low load BFR exercise did not result in significant circulating changes in EPCs in the post-exercise recovery period and may impair exercise-induced EPC mobilization compared to non-BFR exercise. |
| first_indexed | 2024-12-21T12:33:50Z |
| format | Article |
| id | doaj.art-b4a1e43b12884439befbeefd90a688ff |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-21T12:33:50Z |
| publishDate | 2019-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-b4a1e43b12884439befbeefd90a688ff2022-12-21T19:03:58ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2019-04-011010.3389/fphys.2019.00447441194Blood Flow Restriction Exercise Attenuates the Exercise-Induced Endothelial Progenitor Cell Response in Healthy, Young MenRyan MontgomeryAllan PatersonChris WilliamsonGeraint Florida-JamesMark Daniel RossEndothelial progenitor cells (EPCs) are a vasculogenic subset of progenitors, which play a key role in maintenance of endothelial integrity. These cells are exercise-responsive, and thus exercise may play a key role in vascular repair and maintenance via mobilization of such cells. Blood flow restriction exercise, due to the augmentation of local tissue hypoxia, may promote exercise-induced EPC mobilization. Nine, healthy, young (18–30 years) males participated in the study. Participants undertook 2 trials of single leg knee extensor (KE) exercise, at 60% of thigh occlusion pressure (4 sets at 30% maximal torque) (blood flow restriction; BFR) or non- blood flow restriction (non-BFR), in a fasted state. Blood was taken prior, immediately after, and 30 min after exercise. Blood was used for the quantification of hematopoietic progenitor cells (HPCs: CD34+CD45dim), EPCs (CD34+VEGFR2+/CD34+CD45dimVEGFR2+) by flow cytometry. Our results show that unilateral KE exercise did not affect circulating HPC levels (p = 0.856), but did result in increases in both CD34+VEGFR2+ and CD34+CD45dimVEGFR2+ EPCs, but only in the non-BFR trial (CD34+VEGFR2+: 269 ± 42 cells mL-1 to 573 ± 90 cells mL-1, pre- to immediately post-exercise, p = 0.008; CD34+CD45dimVEGFR2+: 129 ± 21 cells mL-1 to 313 ± 103 cells mL-1, pre- to 30 min post-exercise, p = 0.010). In conclusion, low load BFR exercise did not result in significant circulating changes in EPCs in the post-exercise recovery period and may impair exercise-induced EPC mobilization compared to non-BFR exercise.https://www.frontiersin.org/article/10.3389/fphys.2019.00447/fullendothelial progenitorsexerciseendothelialangiogenesisblood flow restricted exercise |
| spellingShingle | Ryan Montgomery Allan Paterson Chris Williamson Geraint Florida-James Mark Daniel Ross Blood Flow Restriction Exercise Attenuates the Exercise-Induced Endothelial Progenitor Cell Response in Healthy, Young Men Frontiers in Physiology endothelial progenitors exercise endothelial angiogenesis blood flow restricted exercise |
| title | Blood Flow Restriction Exercise Attenuates the Exercise-Induced Endothelial Progenitor Cell Response in Healthy, Young Men |
| title_full | Blood Flow Restriction Exercise Attenuates the Exercise-Induced Endothelial Progenitor Cell Response in Healthy, Young Men |
| title_fullStr | Blood Flow Restriction Exercise Attenuates the Exercise-Induced Endothelial Progenitor Cell Response in Healthy, Young Men |
| title_full_unstemmed | Blood Flow Restriction Exercise Attenuates the Exercise-Induced Endothelial Progenitor Cell Response in Healthy, Young Men |
| title_short | Blood Flow Restriction Exercise Attenuates the Exercise-Induced Endothelial Progenitor Cell Response in Healthy, Young Men |
| title_sort | blood flow restriction exercise attenuates the exercise induced endothelial progenitor cell response in healthy young men |
| topic | endothelial progenitors exercise endothelial angiogenesis blood flow restricted exercise |
| url | https://www.frontiersin.org/article/10.3389/fphys.2019.00447/full |
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