Optimization of Extraction of Inulin from Taraxacum kok-saghyz Rodin by Response Surface Methodology and Its MALDI-TOF MS Analysis
Using Taraxacum kok-saghyz Rodin root as raw material, the effects of ultrasonic power, extraction time, ultrasonic temperature and liquid-solid ratio on the extraction rate of inulin from Taraxacum kok-saghyz Rodin root were studied based on single factor experiment and response surface experiment....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-01-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021040173 |
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| author | Jiangyan CHEN Weitao WANG Yiyang DONG Jichuan ZHANG Kongxin HU Qiang MA |
| author_facet | Jiangyan CHEN Weitao WANG Yiyang DONG Jichuan ZHANG Kongxin HU Qiang MA |
| author_sort | Jiangyan CHEN |
| collection | DOAJ |
| description | Using Taraxacum kok-saghyz Rodin root as raw material, the effects of ultrasonic power, extraction time, ultrasonic temperature and liquid-solid ratio on the extraction rate of inulin from Taraxacum kok-saghyz Rodin root were studied based on single factor experiment and response surface experiment. The optimum extraction conditions of inulin from Taraxacum kok-saghyz Rodin root were as follows: Ultrasonic power 230 W, ultrasonic time 34 min, ultrasonic temperature 61 ℃, liquid-solid ratio 30:1 (mL:g), and the extraction rate of inulin was 20.14%±0.19%. The calcium hydroxide-phosphoric acid method, trichloroacetic acid method and Sevage method were used for protein purification, and the results showed that the calcium hydroxide-phosphoric acid method had the best effect, with a protein clearance rate of 90.78% and an inulin loss rate of 26.44%. Inulin with a purity of 80.8% was obtained after protein purification, decolorization, alcohol precipitation and vacuum freeze drying, was a white solid powder. The obtained inulin was characterized and analyzed by MALDI-TOF MS, which determined that the molecular weight of the monomer was 162, the molecular weight of the end group was 179, and the degree of polymerization can be detected in the range of 2 to 30. This study provides a theoretical basis for the comprehensive utilization of Taraxacum kok-saghyz Rodin. |
| first_indexed | 2024-04-11T17:37:01Z |
| format | Article |
| id | doaj.art-b4a56f5f6b43446d84eeb9e0897ab54d |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T17:37:01Z |
| publishDate | 2022-01-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-b4a56f5f6b43446d84eeb9e0897ab54d2022-12-22T04:11:35ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-01-0143120521210.13386/j.issn1002-0306.20210401732021040173-1Optimization of Extraction of Inulin from Taraxacum kok-saghyz Rodin by Response Surface Methodology and Its MALDI-TOF MS AnalysisJiangyan CHEN0Weitao WANG1Yiyang DONG2Jichuan ZHANG3Kongxin HU4Qiang MA5College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, ChinaCollege of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, ChinaInstitute of Health Inspection and Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100176, ChinaInstitute of Industrial and Consumer Product Safety, Chinese Academy of Inspection and Quarantine, Beijing 100176, ChinaUsing Taraxacum kok-saghyz Rodin root as raw material, the effects of ultrasonic power, extraction time, ultrasonic temperature and liquid-solid ratio on the extraction rate of inulin from Taraxacum kok-saghyz Rodin root were studied based on single factor experiment and response surface experiment. The optimum extraction conditions of inulin from Taraxacum kok-saghyz Rodin root were as follows: Ultrasonic power 230 W, ultrasonic time 34 min, ultrasonic temperature 61 ℃, liquid-solid ratio 30:1 (mL:g), and the extraction rate of inulin was 20.14%±0.19%. The calcium hydroxide-phosphoric acid method, trichloroacetic acid method and Sevage method were used for protein purification, and the results showed that the calcium hydroxide-phosphoric acid method had the best effect, with a protein clearance rate of 90.78% and an inulin loss rate of 26.44%. Inulin with a purity of 80.8% was obtained after protein purification, decolorization, alcohol precipitation and vacuum freeze drying, was a white solid powder. The obtained inulin was characterized and analyzed by MALDI-TOF MS, which determined that the molecular weight of the monomer was 162, the molecular weight of the end group was 179, and the degree of polymerization can be detected in the range of 2 to 30. This study provides a theoretical basis for the comprehensive utilization of Taraxacum kok-saghyz Rodin.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021040173taraxacum kok-saghyz rodininulinextractionresponse surfacepurificationmatrix-assisted laser desorption/ionization time-of-flight mass spectrometry(maldi-tof ms) |
| spellingShingle | Jiangyan CHEN Weitao WANG Yiyang DONG Jichuan ZHANG Kongxin HU Qiang MA Optimization of Extraction of Inulin from Taraxacum kok-saghyz Rodin by Response Surface Methodology and Its MALDI-TOF MS Analysis Shipin gongye ke-ji taraxacum kok-saghyz rodin inulin extraction response surface purification matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(maldi-tof ms) |
| title | Optimization of Extraction of Inulin from Taraxacum kok-saghyz Rodin by Response Surface Methodology and Its MALDI-TOF MS Analysis |
| title_full | Optimization of Extraction of Inulin from Taraxacum kok-saghyz Rodin by Response Surface Methodology and Its MALDI-TOF MS Analysis |
| title_fullStr | Optimization of Extraction of Inulin from Taraxacum kok-saghyz Rodin by Response Surface Methodology and Its MALDI-TOF MS Analysis |
| title_full_unstemmed | Optimization of Extraction of Inulin from Taraxacum kok-saghyz Rodin by Response Surface Methodology and Its MALDI-TOF MS Analysis |
| title_short | Optimization of Extraction of Inulin from Taraxacum kok-saghyz Rodin by Response Surface Methodology and Its MALDI-TOF MS Analysis |
| title_sort | optimization of extraction of inulin from taraxacum kok saghyz rodin by response surface methodology and its maldi tof ms analysis |
| topic | taraxacum kok-saghyz rodin inulin extraction response surface purification matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(maldi-tof ms) |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021040173 |
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