Ecological and morphological differentiation among COI haplotype groups in the plant parasitic nematode species Mesocriconema xenoplax
DNA barcoding with the mitochondrial COI gene reveals distinct haplotype subgroups within the monophyletic and parthenogenetic nematode species, Mesocriconema xenoplax. Biological attributes of these haplotype groups (HG) have not been explored. An analysis of M. xenoplax from 40 North American site...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2022-05-01
|
| Series: | Journal of Nematology |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/jofnem-2022-0009 |
| _version_ | 1797405127647166464 |
|---|---|
| author | Matczyszyn Julianne N. Harris Timothy Powers Kirsten Everhart Sydney E. Powers Thomas O. |
| author_facet | Matczyszyn Julianne N. Harris Timothy Powers Kirsten Everhart Sydney E. Powers Thomas O. |
| author_sort | Matczyszyn Julianne N. |
| collection | DOAJ |
| description | DNA barcoding with the mitochondrial COI gene reveals distinct haplotype subgroups within the monophyletic and parthenogenetic nematode species, Mesocriconema xenoplax. Biological attributes of these haplotype groups (HG) have not been explored. An analysis of M. xenoplax from 40 North American sites representing both native plant communities and agroecosystems was conducted to identify possible subgroup associations with ecological, physiological, or geographic factors. A dataset of 132 M. xenoplax specimens was used to generate sequences of a 712 bp region of the cytochrome oxidase subunit I gene. Maximum-likelihood and Bayesian phylogenies recognized seven COI HG (≥99/0.99 posterior probability/bootstrap value). Species delimitation metrics largely supported the genetic integrity of the HG. Discriminant function analysis of HG morphological traits identified stylet length, total body length, and stylet knob width as the strongest distinguishing features among the seven groups, with stylet length as the strongest single distinguishing morphological feature. Multivariate analysis identified land cover, ecoregion, and maximum temperature as predictors of 53.6% of the total variation (P = 0.001). Within land cover, HG categorized under “herbaceous,” “woody wetlands,” and “deciduous forest” were distinct in DAPC and RDA analyses and were significantly different (analysis of molecular variance P = 0.001). These results provide empirical evidence for molecular, morphological, and ecological differentiation associated with HG within the monophyletic clade that represents the species Mesocriconema xenoplax. |
| first_indexed | 2024-03-09T03:05:15Z |
| format | Article |
| id | doaj.art-b4a641480360483da727e0fbaa7b4a14 |
| institution | Directory Open Access Journal |
| issn | 2640-396X |
| language | English |
| last_indexed | 2024-03-09T03:05:15Z |
| publishDate | 2022-05-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Journal of Nematology |
| spelling | doaj.art-b4a641480360483da727e0fbaa7b4a142023-12-04T08:04:12ZengSciendoJournal of Nematology2640-396X2022-05-0154119921310.2478/jofnem-2022-0009Ecological and morphological differentiation among COI haplotype groups in the plant parasitic nematode species Mesocriconema xenoplaxMatczyszyn Julianne N.0Harris Timothy1Powers Kirsten2Everhart Sydney E.3Powers Thomas O.4Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE, 68583-0722Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE, 68583-0722Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE, 68583-0722Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT06269-4067Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE, 68583-0722DNA barcoding with the mitochondrial COI gene reveals distinct haplotype subgroups within the monophyletic and parthenogenetic nematode species, Mesocriconema xenoplax. Biological attributes of these haplotype groups (HG) have not been explored. An analysis of M. xenoplax from 40 North American sites representing both native plant communities and agroecosystems was conducted to identify possible subgroup associations with ecological, physiological, or geographic factors. A dataset of 132 M. xenoplax specimens was used to generate sequences of a 712 bp region of the cytochrome oxidase subunit I gene. Maximum-likelihood and Bayesian phylogenies recognized seven COI HG (≥99/0.99 posterior probability/bootstrap value). Species delimitation metrics largely supported the genetic integrity of the HG. Discriminant function analysis of HG morphological traits identified stylet length, total body length, and stylet knob width as the strongest distinguishing features among the seven groups, with stylet length as the strongest single distinguishing morphological feature. Multivariate analysis identified land cover, ecoregion, and maximum temperature as predictors of 53.6% of the total variation (P = 0.001). Within land cover, HG categorized under “herbaceous,” “woody wetlands,” and “deciduous forest” were distinct in DAPC and RDA analyses and were significantly different (analysis of molecular variance P = 0.001). These results provide empirical evidence for molecular, morphological, and ecological differentiation associated with HG within the monophyletic clade that represents the species Mesocriconema xenoplax.https://doi.org/10.2478/jofnem-2022-0009parthenogenetic nematodespopulation structurespatial genetic structurespecies delimitationsystematicstaxonomyterrestrial nematodes |
| spellingShingle | Matczyszyn Julianne N. Harris Timothy Powers Kirsten Everhart Sydney E. Powers Thomas O. Ecological and morphological differentiation among COI haplotype groups in the plant parasitic nematode species Mesocriconema xenoplax Journal of Nematology parthenogenetic nematodes population structure spatial genetic structure species delimitation systematics taxonomy terrestrial nematodes |
| title | Ecological and morphological differentiation among COI haplotype groups in the plant parasitic nematode species Mesocriconema xenoplax |
| title_full | Ecological and morphological differentiation among COI haplotype groups in the plant parasitic nematode species Mesocriconema xenoplax |
| title_fullStr | Ecological and morphological differentiation among COI haplotype groups in the plant parasitic nematode species Mesocriconema xenoplax |
| title_full_unstemmed | Ecological and morphological differentiation among COI haplotype groups in the plant parasitic nematode species Mesocriconema xenoplax |
| title_short | Ecological and morphological differentiation among COI haplotype groups in the plant parasitic nematode species Mesocriconema xenoplax |
| title_sort | ecological and morphological differentiation among coi haplotype groups in the plant parasitic nematode species mesocriconema xenoplax |
| topic | parthenogenetic nematodes population structure spatial genetic structure species delimitation systematics taxonomy terrestrial nematodes |
| url | https://doi.org/10.2478/jofnem-2022-0009 |
| work_keys_str_mv | AT matczyszynjuliannen ecologicalandmorphologicaldifferentiationamongcoihaplotypegroupsintheplantparasiticnematodespeciesmesocriconemaxenoplax AT harristimothy ecologicalandmorphologicaldifferentiationamongcoihaplotypegroupsintheplantparasiticnematodespeciesmesocriconemaxenoplax AT powerskirsten ecologicalandmorphologicaldifferentiationamongcoihaplotypegroupsintheplantparasiticnematodespeciesmesocriconemaxenoplax AT everhartsydneye ecologicalandmorphologicaldifferentiationamongcoihaplotypegroupsintheplantparasiticnematodespeciesmesocriconemaxenoplax AT powersthomaso ecologicalandmorphologicaldifferentiationamongcoihaplotypegroupsintheplantparasiticnematodespeciesmesocriconemaxenoplax |