Effects of Dietary Ochratoxin A on Growth Performance and Intestinal Apical Junctional Complex of Juvenile Grass Carp (<i>Ctenopharyngodon idella</i>)
Ochratoxin A (OTA) contamination widely occurs in various feed ingredients and food crops, potentially posing a serious health threat to animals. In this research, 1260 juvenile grass carp were separately fed with seven distinct experimental diets (0, 406, 795, 1209, 1612, 2003 and 2406 µg of OTA/kg...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/13/1/11 |
| _version_ | 1797543651130212352 |
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| author | Xin Liu Pei Wu Wei-Dan Jiang Yang Liu Jun Jiang Sheng-Yao Kuang Ling Tang Xiao-Qiu Zhou Lin Feng |
| author_facet | Xin Liu Pei Wu Wei-Dan Jiang Yang Liu Jun Jiang Sheng-Yao Kuang Ling Tang Xiao-Qiu Zhou Lin Feng |
| author_sort | Xin Liu |
| collection | DOAJ |
| description | Ochratoxin A (OTA) contamination widely occurs in various feed ingredients and food crops, potentially posing a serious health threat to animals. In this research, 1260 juvenile grass carp were separately fed with seven distinct experimental diets (0, 406, 795, 1209, 1612, 2003 and 2406 µg of OTA/kg of diet) for 60 consecutive days to evaluate OTA’s toxic effect on the intestinal apical junctional complex (including the tight junction (TJ) and the adherents junction (AJ)) and the underlying action mechanisms. Our experiment firstly confirmed that OTA caused fish growth retardation and disrupted the intestinal structural integrity. The detailed results show that OTA (1) depressed the feed efficiency, percentage weight gain and specific growth rate; (2) accumulated in the intestine; (3) caused oxidative damage and increased intestinal permeability; and (4) induced the RhoA/ROCK signaling pathway, destroying intestinal apical junctional complexes. Notably, OTA intervention did not result in changes in the gene expression of claudin-3c (in the proximal intestine (PI)), claudin-b and ZO-2b (in the mid intestine (MI) and distal intestine (DI)) in the fish intestine. |
| first_indexed | 2024-03-10T13:48:39Z |
| format | Article |
| id | doaj.art-b4a6911eb28e4c3f875d65cfa5054613 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T13:48:39Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-b4a6911eb28e4c3f875d65cfa50546132023-11-21T02:25:15ZengMDPI AGToxins2072-66512020-12-011311110.3390/toxins13010011Effects of Dietary Ochratoxin A on Growth Performance and Intestinal Apical Junctional Complex of Juvenile Grass Carp (<i>Ctenopharyngodon idella</i>)Xin Liu0Pei Wu1Wei-Dan Jiang2Yang Liu3Jun Jiang4Sheng-Yao Kuang5Ling Tang6Xiao-Qiu Zhou7Lin Feng8Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaAnimal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaAnimal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaAnimal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaAnimal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaAnimal Nutrition Institute, Sichuan Academy of Animal Science, Sichuan Animtech Feed. Co., Ltd., Chengdu 610066, ChinaAnimal Nutrition Institute, Sichuan Academy of Animal Science, Sichuan Animtech Feed. Co., Ltd., Chengdu 610066, ChinaAnimal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaAnimal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, ChinaOchratoxin A (OTA) contamination widely occurs in various feed ingredients and food crops, potentially posing a serious health threat to animals. In this research, 1260 juvenile grass carp were separately fed with seven distinct experimental diets (0, 406, 795, 1209, 1612, 2003 and 2406 µg of OTA/kg of diet) for 60 consecutive days to evaluate OTA’s toxic effect on the intestinal apical junctional complex (including the tight junction (TJ) and the adherents junction (AJ)) and the underlying action mechanisms. Our experiment firstly confirmed that OTA caused fish growth retardation and disrupted the intestinal structural integrity. The detailed results show that OTA (1) depressed the feed efficiency, percentage weight gain and specific growth rate; (2) accumulated in the intestine; (3) caused oxidative damage and increased intestinal permeability; and (4) induced the RhoA/ROCK signaling pathway, destroying intestinal apical junctional complexes. Notably, OTA intervention did not result in changes in the gene expression of claudin-3c (in the proximal intestine (PI)), claudin-b and ZO-2b (in the mid intestine (MI) and distal intestine (DI)) in the fish intestine.https://www.mdpi.com/2072-6651/13/1/11ochratoxin Aintestineapical junctional complextight junctionadhesive junctionjuvenile grass carp (<i>Ctenopharyngodon idella</i>) |
| spellingShingle | Xin Liu Pei Wu Wei-Dan Jiang Yang Liu Jun Jiang Sheng-Yao Kuang Ling Tang Xiao-Qiu Zhou Lin Feng Effects of Dietary Ochratoxin A on Growth Performance and Intestinal Apical Junctional Complex of Juvenile Grass Carp (<i>Ctenopharyngodon idella</i>) Toxins ochratoxin A intestine apical junctional complex tight junction adhesive junction juvenile grass carp (<i>Ctenopharyngodon idella</i>) |
| title | Effects of Dietary Ochratoxin A on Growth Performance and Intestinal Apical Junctional Complex of Juvenile Grass Carp (<i>Ctenopharyngodon idella</i>) |
| title_full | Effects of Dietary Ochratoxin A on Growth Performance and Intestinal Apical Junctional Complex of Juvenile Grass Carp (<i>Ctenopharyngodon idella</i>) |
| title_fullStr | Effects of Dietary Ochratoxin A on Growth Performance and Intestinal Apical Junctional Complex of Juvenile Grass Carp (<i>Ctenopharyngodon idella</i>) |
| title_full_unstemmed | Effects of Dietary Ochratoxin A on Growth Performance and Intestinal Apical Junctional Complex of Juvenile Grass Carp (<i>Ctenopharyngodon idella</i>) |
| title_short | Effects of Dietary Ochratoxin A on Growth Performance and Intestinal Apical Junctional Complex of Juvenile Grass Carp (<i>Ctenopharyngodon idella</i>) |
| title_sort | effects of dietary ochratoxin a on growth performance and intestinal apical junctional complex of juvenile grass carp i ctenopharyngodon idella i |
| topic | ochratoxin A intestine apical junctional complex tight junction adhesive junction juvenile grass carp (<i>Ctenopharyngodon idella</i>) |
| url | https://www.mdpi.com/2072-6651/13/1/11 |
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