Molecular Characterization of Tropomyosin and Its Potential Involvement in Muscle Contraction in Pacific Abalone
Tropomyosin (TPM) is a contractile protein responsible for muscle contraction through its actin-binding activity. The complete sequence of <i>TPM</i> in <i>Haliotis discus hannai</i> (<i>Hdh-TPM</i>) was 2160 bp, encoding 284 amino acids, and contained a TPM signa...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/1/2 |
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| author | Md Abu Hanif Shaharior Hossen Won Kyo Lee Kang Hee Kho |
| author_facet | Md Abu Hanif Shaharior Hossen Won Kyo Lee Kang Hee Kho |
| author_sort | Md Abu Hanif |
| collection | DOAJ |
| description | Tropomyosin (TPM) is a contractile protein responsible for muscle contraction through its actin-binding activity. The complete sequence of <i>TPM</i> in <i>Haliotis discus hannai</i> (<i>Hdh-TPM</i>) was 2160 bp, encoding 284 amino acids, and contained a TPM signature motif and a TPM domain. Gene ontology (GO) analysis based on the amino acid sequence predicted Hdh-TPM to have an actin-binding function in the cytoskeleton. The 3D analysis predicted the Hdh-TPM to have a coiled-coil α-helical structure. Phylogenetically, Hdh-TPM formed a cluster with other TPM/TPM1 proteins during analysis. The tissue-specific mRNA expression analysis found the higher expression of <i>Hdh-TPM</i> in the heart and muscles; however, during embryonic and larval development (ELD), the higher expression was found in the trochophore larvae and veliger larvae. <i>Hdh-TPM</i> expression was upregulated in fast-growing abalone. Increasing thermal stress over a long period decreased <i>Hdh-TPM</i> expression. Long-term starvation (>1 week) reduced the mRNA expression of <i>Hdh-TPM</i> in muscle; however, the mRNA expression of <i>Hdh-TPM</i> was significantly higher in the mantle, which may indicate overexpression. This study is the first comprehensive study to characterize the <i>Hdh-TPM</i> gene in Pacific abalone and to report the expression of <i>Hdh-TPM</i> in different organs, and during ELD, different growth patterns, thermal stress, seasonal changes, and starvation. |
| first_indexed | 2024-03-09T12:38:11Z |
| format | Article |
| id | doaj.art-b4a9f0a651084999871f20bf15f1cf81 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T12:38:11Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-b4a9f0a651084999871f20bf15f1cf812023-11-30T22:22:05ZengMDPI AGGenes2073-44252022-12-01141210.3390/genes14010002Molecular Characterization of Tropomyosin and Its Potential Involvement in Muscle Contraction in Pacific AbaloneMd Abu Hanif0Shaharior Hossen1Won Kyo Lee2Kang Hee Kho3Department of Fisheries Science, Chonnam National University, Yeosu 59626, Republic of KoreaDepartment of Fisheries Science, Chonnam National University, Yeosu 59626, Republic of KoreaDepartment of Fisheries Science, Chonnam National University, Yeosu 59626, Republic of KoreaDepartment of Fisheries Science, Chonnam National University, Yeosu 59626, Republic of KoreaTropomyosin (TPM) is a contractile protein responsible for muscle contraction through its actin-binding activity. The complete sequence of <i>TPM</i> in <i>Haliotis discus hannai</i> (<i>Hdh-TPM</i>) was 2160 bp, encoding 284 amino acids, and contained a TPM signature motif and a TPM domain. Gene ontology (GO) analysis based on the amino acid sequence predicted Hdh-TPM to have an actin-binding function in the cytoskeleton. The 3D analysis predicted the Hdh-TPM to have a coiled-coil α-helical structure. Phylogenetically, Hdh-TPM formed a cluster with other TPM/TPM1 proteins during analysis. The tissue-specific mRNA expression analysis found the higher expression of <i>Hdh-TPM</i> in the heart and muscles; however, during embryonic and larval development (ELD), the higher expression was found in the trochophore larvae and veliger larvae. <i>Hdh-TPM</i> expression was upregulated in fast-growing abalone. Increasing thermal stress over a long period decreased <i>Hdh-TPM</i> expression. Long-term starvation (>1 week) reduced the mRNA expression of <i>Hdh-TPM</i> in muscle; however, the mRNA expression of <i>Hdh-TPM</i> was significantly higher in the mantle, which may indicate overexpression. This study is the first comprehensive study to characterize the <i>Hdh-TPM</i> gene in Pacific abalone and to report the expression of <i>Hdh-TPM</i> in different organs, and during ELD, different growth patterns, thermal stress, seasonal changes, and starvation.https://www.mdpi.com/2073-4425/14/1/2cloninggene ontologycoiled-coil structureexpression analysiscontractile function |
| spellingShingle | Md Abu Hanif Shaharior Hossen Won Kyo Lee Kang Hee Kho Molecular Characterization of Tropomyosin and Its Potential Involvement in Muscle Contraction in Pacific Abalone Genes cloning gene ontology coiled-coil structure expression analysis contractile function |
| title | Molecular Characterization of Tropomyosin and Its Potential Involvement in Muscle Contraction in Pacific Abalone |
| title_full | Molecular Characterization of Tropomyosin and Its Potential Involvement in Muscle Contraction in Pacific Abalone |
| title_fullStr | Molecular Characterization of Tropomyosin and Its Potential Involvement in Muscle Contraction in Pacific Abalone |
| title_full_unstemmed | Molecular Characterization of Tropomyosin and Its Potential Involvement in Muscle Contraction in Pacific Abalone |
| title_short | Molecular Characterization of Tropomyosin and Its Potential Involvement in Muscle Contraction in Pacific Abalone |
| title_sort | molecular characterization of tropomyosin and its potential involvement in muscle contraction in pacific abalone |
| topic | cloning gene ontology coiled-coil structure expression analysis contractile function |
| url | https://www.mdpi.com/2073-4425/14/1/2 |
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