Silicon application and related changes in soil bacterial community dynamics reduced ginseng black spot incidence in Panax ginseng in a short-term study
Abstract Background This study analyzed the effect of silicon (Si) application on the occurrence of ginseng black spot caused by Alternaria panax. We explored the differences in soil physical and chemical factors and microbial community structure following Si application as well as the key factors t...
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| Format: | Article |
| Language: | English |
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BMC
2019-11-01
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| Series: | BMC Microbiology |
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| Online Access: | http://link.springer.com/article/10.1186/s12866-019-1627-z |
| _version_ | 1818903427046965248 |
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| author | Meijia Li Qiuxia Wang Zhengbo Liu Xiaoxi Pan Yayu Zhang |
| author_facet | Meijia Li Qiuxia Wang Zhengbo Liu Xiaoxi Pan Yayu Zhang |
| author_sort | Meijia Li |
| collection | DOAJ |
| description | Abstract Background This study analyzed the effect of silicon (Si) application on the occurrence of ginseng black spot caused by Alternaria panax. We explored the differences in soil physical and chemical factors and microbial community structure following Si application as well as the key factors that affected the occurrence of ginseng black spot in soil. Potted Panax ginseng plants were used to assess the effect of Si treatment on ginseng black spot. Soil physical and chemical properties were comprehensively analyzed. Bacterial communities were analyzed using Illumina HiSeq sequencing targeting the 16S rRNA gene. Results After inoculation with A. panax, the morbidity (and morbidity index) of ginseng with and without Si was 52% (46) and 83% (77), respectively. Soil physical and chemical analysis showed that under the ginseng black spot inoculation, bacterial communities were mainly affected by pH and available potassium, followed by ammonium nitrogen and available Si. NMDS and PLS-DA analyses and the heat maps of relative abundance revealed that Si application elevated the resistance of ginseng black spot as regulated by the abundance and diversity of bacterial flora in rhizosphere soils. Heatmap analysis at the genus level revealed that A. panax + Si inoculations significantly increased the soil community abundance of Sandaracinus, Polycyclovorans, Hirschia, Haliangium, Nitrospira, Saccharothrix, Aeromicrobium, Luteimonas, and Rubellimicrobium and led to a bacterial community structure with relative abundances that were significantly similar to that of untreated soil. Conclusions Short-term Si application also significantly regulated the structural impact on soil microorganisms caused by ginseng black spot. Our findings indicated that Si applications may possibly be used in the prevention and treatment of ginseng black spot. |
| first_indexed | 2024-12-19T20:51:22Z |
| format | Article |
| id | doaj.art-b4abff4149784fca84d70257ffe9f72a |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-19T20:51:22Z |
| publishDate | 2019-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-b4abff4149784fca84d70257ffe9f72a2022-12-21T20:06:06ZengBMCBMC Microbiology1471-21802019-11-0119111210.1186/s12866-019-1627-zSilicon application and related changes in soil bacterial community dynamics reduced ginseng black spot incidence in Panax ginseng in a short-term studyMeijia Li0Qiuxia Wang1Zhengbo Liu2Xiaoxi Pan3Yayu Zhang4Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agriculture SciencesInstitute of Special Wild Economic Animals and Plants, Chinese Academy of Agriculture SciencesInstitute of Special Wild Economic Animals and Plants, Chinese Academy of Agriculture SciencesInstitute of Special Wild Economic Animals and Plants, Chinese Academy of Agriculture SciencesInstitute of Special Wild Economic Animals and Plants, Chinese Academy of Agriculture SciencesAbstract Background This study analyzed the effect of silicon (Si) application on the occurrence of ginseng black spot caused by Alternaria panax. We explored the differences in soil physical and chemical factors and microbial community structure following Si application as well as the key factors that affected the occurrence of ginseng black spot in soil. Potted Panax ginseng plants were used to assess the effect of Si treatment on ginseng black spot. Soil physical and chemical properties were comprehensively analyzed. Bacterial communities were analyzed using Illumina HiSeq sequencing targeting the 16S rRNA gene. Results After inoculation with A. panax, the morbidity (and morbidity index) of ginseng with and without Si was 52% (46) and 83% (77), respectively. Soil physical and chemical analysis showed that under the ginseng black spot inoculation, bacterial communities were mainly affected by pH and available potassium, followed by ammonium nitrogen and available Si. NMDS and PLS-DA analyses and the heat maps of relative abundance revealed that Si application elevated the resistance of ginseng black spot as regulated by the abundance and diversity of bacterial flora in rhizosphere soils. Heatmap analysis at the genus level revealed that A. panax + Si inoculations significantly increased the soil community abundance of Sandaracinus, Polycyclovorans, Hirschia, Haliangium, Nitrospira, Saccharothrix, Aeromicrobium, Luteimonas, and Rubellimicrobium and led to a bacterial community structure with relative abundances that were significantly similar to that of untreated soil. Conclusions Short-term Si application also significantly regulated the structural impact on soil microorganisms caused by ginseng black spot. Our findings indicated that Si applications may possibly be used in the prevention and treatment of ginseng black spot.http://link.springer.com/article/10.1186/s12866-019-1627-zAlternaria panaxSiliconGinseng black spotSoil bacterial communityPanax ginsengIllumina HiSeq sequencing |
| spellingShingle | Meijia Li Qiuxia Wang Zhengbo Liu Xiaoxi Pan Yayu Zhang Silicon application and related changes in soil bacterial community dynamics reduced ginseng black spot incidence in Panax ginseng in a short-term study BMC Microbiology Alternaria panax Silicon Ginseng black spot Soil bacterial community Panax ginseng Illumina HiSeq sequencing |
| title | Silicon application and related changes in soil bacterial community dynamics reduced ginseng black spot incidence in Panax ginseng in a short-term study |
| title_full | Silicon application and related changes in soil bacterial community dynamics reduced ginseng black spot incidence in Panax ginseng in a short-term study |
| title_fullStr | Silicon application and related changes in soil bacterial community dynamics reduced ginseng black spot incidence in Panax ginseng in a short-term study |
| title_full_unstemmed | Silicon application and related changes in soil bacterial community dynamics reduced ginseng black spot incidence in Panax ginseng in a short-term study |
| title_short | Silicon application and related changes in soil bacterial community dynamics reduced ginseng black spot incidence in Panax ginseng in a short-term study |
| title_sort | silicon application and related changes in soil bacterial community dynamics reduced ginseng black spot incidence in panax ginseng in a short term study |
| topic | Alternaria panax Silicon Ginseng black spot Soil bacterial community Panax ginseng Illumina HiSeq sequencing |
| url | http://link.springer.com/article/10.1186/s12866-019-1627-z |
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