Retinal Progenitor Cell Xenografts to the Pig Retina: Immunological Reactions
We evaluated the host response to murine retinal progenitor cells (RPCs) following transplantation to the subretinal space (SRS) of the pig. RPCs from GFP mice were transplanted subretinally in 18 nonimmunosuppressed normal or laser-treated pigs. Evaluation of the SRS was performed on hematoxylin-eo...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2006-08-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/000000006783981594 |
| _version_ | 1819205229823918080 |
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| author | Karin Warfvinge Jens F. Kiilgaard Henry Klassen Parisa Zamiri Erik Scherfig Wayne Streilein Jan U. Prause Michael J. Young Ph.D. |
| author_facet | Karin Warfvinge Jens F. Kiilgaard Henry Klassen Parisa Zamiri Erik Scherfig Wayne Streilein Jan U. Prause Michael J. Young Ph.D. |
| author_sort | Karin Warfvinge |
| collection | DOAJ |
| description | We evaluated the host response to murine retinal progenitor cells (RPCs) following transplantation to the subretinal space (SRS) of the pig. RPCs from GFP mice were transplanted subretinally in 18 nonimmunosuppressed normal or laser-treated pigs. Evaluation of the SRS was performed on hematoxylin-eosin (H&E)-stained sections. Serum samples were taken from naive and RPC-grafted pigs and mouse-reactive antibody responses were assessed. At 1 week, histology showed a few perivascular lymphocytes consistent with a mild retinal vasculitis, and depigmentation of the RPE with large numbers of mononuclear inflammatory cells in the choroid near the transplantation site. Large choroidal infiltrates were evident at 2–5 weeks. Serum from naive and RPC-xenografted pigs contained significant levels of preformed IgG and IgM antibodies against murine antigens. Xenogeneic RPCs transplanted to the porcine SRS induced mononuclear infiltration in the choroid with graft rejection occurring over 2–5 weeks. Serum analysis confirmed that mice and pigs are discordant species; however, a cell-mediated acute mechanism appears to be responsible, rather than an antibody-mediated rejection. |
| first_indexed | 2024-12-23T04:48:23Z |
| format | Article |
| id | doaj.art-b4acf4236d9c4586bfa7e325494083a9 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-23T04:48:23Z |
| publishDate | 2006-08-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-b4acf4236d9c4586bfa7e325494083a92022-12-21T17:59:33ZengSAGE PublishingCell Transplantation0963-68971555-38922006-08-011510.3727/000000006783981594Retinal Progenitor Cell Xenografts to the Pig Retina: Immunological ReactionsKarin Warfvinge0Jens F. Kiilgaard1Henry Klassen2Parisa Zamiri3Erik Scherfig4Wayne Streilein5Jan U. Prause6Michael J. Young Ph.D.7Department of Ophthalmology, Lund University Hospital, Lund, SwedenEye Department, Rigshospitalet and Eye Pathology Institute, Copenhagen University, Copenhagen, Denmark Singapore Eye Research Institute, Singapore.Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA, USAEye Department, Rigshospitalet and Eye Pathology Institute, Copenhagen University, Copenhagen, DenmarkSchepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA, USADepartment of Ophthalmology, UC Irvine, Irvine, CA, USASchepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA, USAWe evaluated the host response to murine retinal progenitor cells (RPCs) following transplantation to the subretinal space (SRS) of the pig. RPCs from GFP mice were transplanted subretinally in 18 nonimmunosuppressed normal or laser-treated pigs. Evaluation of the SRS was performed on hematoxylin-eosin (H&E)-stained sections. Serum samples were taken from naive and RPC-grafted pigs and mouse-reactive antibody responses were assessed. At 1 week, histology showed a few perivascular lymphocytes consistent with a mild retinal vasculitis, and depigmentation of the RPE with large numbers of mononuclear inflammatory cells in the choroid near the transplantation site. Large choroidal infiltrates were evident at 2–5 weeks. Serum from naive and RPC-xenografted pigs contained significant levels of preformed IgG and IgM antibodies against murine antigens. Xenogeneic RPCs transplanted to the porcine SRS induced mononuclear infiltration in the choroid with graft rejection occurring over 2–5 weeks. Serum analysis confirmed that mice and pigs are discordant species; however, a cell-mediated acute mechanism appears to be responsible, rather than an antibody-mediated rejection.https://doi.org/10.3727/000000006783981594 |
| spellingShingle | Karin Warfvinge Jens F. Kiilgaard Henry Klassen Parisa Zamiri Erik Scherfig Wayne Streilein Jan U. Prause Michael J. Young Ph.D. Retinal Progenitor Cell Xenografts to the Pig Retina: Immunological Reactions Cell Transplantation |
| title | Retinal Progenitor Cell Xenografts to the Pig Retina: Immunological Reactions |
| title_full | Retinal Progenitor Cell Xenografts to the Pig Retina: Immunological Reactions |
| title_fullStr | Retinal Progenitor Cell Xenografts to the Pig Retina: Immunological Reactions |
| title_full_unstemmed | Retinal Progenitor Cell Xenografts to the Pig Retina: Immunological Reactions |
| title_short | Retinal Progenitor Cell Xenografts to the Pig Retina: Immunological Reactions |
| title_sort | retinal progenitor cell xenografts to the pig retina immunological reactions |
| url | https://doi.org/10.3727/000000006783981594 |
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