Karrikin Improves Osmotic and Salt Stress Tolerance via the Regulation of the Redox Homeostasis in the Oil Plant Sapium sebiferum
Karrikins are reported to stimulate seed germination, regulate seedling growth, and increase the seedling vigor in abiotic stress conditions in plants. Nevertheless, how karrikins alleviate abiotic stress remains largely elusive. In this study, we found that karrikin (KAR1) could significantly allev...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-03-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.00216/full |
| _version_ | 1818468138412408832 |
|---|---|
| author | Faheem Afzal Shah Xiao Wei Qiaojian Wang Wenbo Liu Dongdong Wang Yuanyuan Yao Hao Hu Xue Chen Shengwei Huang Jinyan Hou Ruiju Lu Chenghong Liu Jun Ni Lifang Wu Lifang Wu |
| author_facet | Faheem Afzal Shah Xiao Wei Qiaojian Wang Wenbo Liu Dongdong Wang Yuanyuan Yao Hao Hu Xue Chen Shengwei Huang Jinyan Hou Ruiju Lu Chenghong Liu Jun Ni Lifang Wu Lifang Wu |
| author_sort | Faheem Afzal Shah |
| collection | DOAJ |
| description | Karrikins are reported to stimulate seed germination, regulate seedling growth, and increase the seedling vigor in abiotic stress conditions in plants. Nevertheless, how karrikins alleviate abiotic stress remains largely elusive. In this study, we found that karrikin (KAR1) could significantly alleviate both drought and salt stress in the important oil plant Sapium sebiferum. KAR1 supplementation in growth medium at a nanomolar (nM) concentration was enough to recover seed germination under salt and osmotic stress conditions. One nanomolar of KAR1 improved seedling biomass, increased the taproot length, and increased the number of lateral roots under abiotic stresses, suggesting that KAR1 is a potent alleviator of abiotic stresses in plants. Under abiotic stresses, KAR1-treated seedlings had a higher activity of the key antioxidative enzymes, such as superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase, in comparison with the control, which leads to a lower level of hydrogen peroxide, malondialdehyde, and electrolyte leakage. Moreover, the metabolome analysis showed that KAR1 treatment significantly increased the level of organic acids and amino acids, which played important roles in redox homeostasis under stresses, suggesting that karrikins might alleviate abiotic stresses via the regulation of redox homeostasis. Under abiotic stresses, applications of karrikins did not increase the endogenous abscisic acid level but altered the expression of several ABA signaling genes, such as SNF1-RELATED PROTEIN KINASE2.3, SNF1-RELATED PROTEIN KINASE2.6, ABI3, and ABI5, suggesting potential interactions between karrikins and ABA signaling in the stress responses. Conclusively, we not only provided the physiological and molecular evidence to clarify the mechanism of karrikins in the regulation of stress adaptation in S. sebiferum but also showed the potential value of karrikins in agricultural practices, which will lay a foundation for further studies about the role of karrikins in abiotic stress alleviation in plants. |
| first_indexed | 2024-04-13T21:09:38Z |
| format | Article |
| id | doaj.art-b4ada5513d694513adffd71205b9145e |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T21:09:38Z |
| publishDate | 2020-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-b4ada5513d694513adffd71205b9145e2022-12-22T02:29:51ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-03-011110.3389/fpls.2020.00216471128Karrikin Improves Osmotic and Salt Stress Tolerance via the Regulation of the Redox Homeostasis in the Oil Plant Sapium sebiferumFaheem Afzal Shah0Xiao Wei1Qiaojian Wang2Wenbo Liu3Dongdong Wang4Yuanyuan Yao5Hao Hu6Xue Chen7Shengwei Huang8Jinyan Hou9Ruiju Lu10Chenghong Liu11Jun Ni12Lifang Wu13Lifang Wu14Key Laboratory of the High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, ChinaKey Laboratory of the High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, ChinaKey Laboratory of the High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, ChinaKey Laboratory of the High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, ChinaKey Laboratory of the High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, ChinaKey Laboratory of the High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, ChinaKey Laboratory of the High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, ChinaKey Laboratory of the High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, ChinaKey Laboratory of the High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, ChinaKey Laboratory of the High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, ChinaBiotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaBiotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaKey Laboratory of the High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, ChinaKey Laboratory of the High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, ChinaTaihe Experimental Station, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Taihe, ChinaKarrikins are reported to stimulate seed germination, regulate seedling growth, and increase the seedling vigor in abiotic stress conditions in plants. Nevertheless, how karrikins alleviate abiotic stress remains largely elusive. In this study, we found that karrikin (KAR1) could significantly alleviate both drought and salt stress in the important oil plant Sapium sebiferum. KAR1 supplementation in growth medium at a nanomolar (nM) concentration was enough to recover seed germination under salt and osmotic stress conditions. One nanomolar of KAR1 improved seedling biomass, increased the taproot length, and increased the number of lateral roots under abiotic stresses, suggesting that KAR1 is a potent alleviator of abiotic stresses in plants. Under abiotic stresses, KAR1-treated seedlings had a higher activity of the key antioxidative enzymes, such as superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase, in comparison with the control, which leads to a lower level of hydrogen peroxide, malondialdehyde, and electrolyte leakage. Moreover, the metabolome analysis showed that KAR1 treatment significantly increased the level of organic acids and amino acids, which played important roles in redox homeostasis under stresses, suggesting that karrikins might alleviate abiotic stresses via the regulation of redox homeostasis. Under abiotic stresses, applications of karrikins did not increase the endogenous abscisic acid level but altered the expression of several ABA signaling genes, such as SNF1-RELATED PROTEIN KINASE2.3, SNF1-RELATED PROTEIN KINASE2.6, ABI3, and ABI5, suggesting potential interactions between karrikins and ABA signaling in the stress responses. Conclusively, we not only provided the physiological and molecular evidence to clarify the mechanism of karrikins in the regulation of stress adaptation in S. sebiferum but also showed the potential value of karrikins in agricultural practices, which will lay a foundation for further studies about the role of karrikins in abiotic stress alleviation in plants.https://www.frontiersin.org/article/10.3389/fpls.2020.00216/fullkarrikinsseed germinationsalt stressosmotic stressredox homeostasisabscisic acid signaling |
| spellingShingle | Faheem Afzal Shah Xiao Wei Qiaojian Wang Wenbo Liu Dongdong Wang Yuanyuan Yao Hao Hu Xue Chen Shengwei Huang Jinyan Hou Ruiju Lu Chenghong Liu Jun Ni Lifang Wu Lifang Wu Karrikin Improves Osmotic and Salt Stress Tolerance via the Regulation of the Redox Homeostasis in the Oil Plant Sapium sebiferum Frontiers in Plant Science karrikins seed germination salt stress osmotic stress redox homeostasis abscisic acid signaling |
| title | Karrikin Improves Osmotic and Salt Stress Tolerance via the Regulation of the Redox Homeostasis in the Oil Plant Sapium sebiferum |
| title_full | Karrikin Improves Osmotic and Salt Stress Tolerance via the Regulation of the Redox Homeostasis in the Oil Plant Sapium sebiferum |
| title_fullStr | Karrikin Improves Osmotic and Salt Stress Tolerance via the Regulation of the Redox Homeostasis in the Oil Plant Sapium sebiferum |
| title_full_unstemmed | Karrikin Improves Osmotic and Salt Stress Tolerance via the Regulation of the Redox Homeostasis in the Oil Plant Sapium sebiferum |
| title_short | Karrikin Improves Osmotic and Salt Stress Tolerance via the Regulation of the Redox Homeostasis in the Oil Plant Sapium sebiferum |
| title_sort | karrikin improves osmotic and salt stress tolerance via the regulation of the redox homeostasis in the oil plant sapium sebiferum |
| topic | karrikins seed germination salt stress osmotic stress redox homeostasis abscisic acid signaling |
| url | https://www.frontiersin.org/article/10.3389/fpls.2020.00216/full |
| work_keys_str_mv | AT faheemafzalshah karrikinimprovesosmoticandsaltstresstoleranceviatheregulationoftheredoxhomeostasisintheoilplantsapiumsebiferum AT xiaowei karrikinimprovesosmoticandsaltstresstoleranceviatheregulationoftheredoxhomeostasisintheoilplantsapiumsebiferum AT qiaojianwang karrikinimprovesosmoticandsaltstresstoleranceviatheregulationoftheredoxhomeostasisintheoilplantsapiumsebiferum AT wenboliu karrikinimprovesosmoticandsaltstresstoleranceviatheregulationoftheredoxhomeostasisintheoilplantsapiumsebiferum AT dongdongwang karrikinimprovesosmoticandsaltstresstoleranceviatheregulationoftheredoxhomeostasisintheoilplantsapiumsebiferum AT yuanyuanyao karrikinimprovesosmoticandsaltstresstoleranceviatheregulationoftheredoxhomeostasisintheoilplantsapiumsebiferum AT haohu karrikinimprovesosmoticandsaltstresstoleranceviatheregulationoftheredoxhomeostasisintheoilplantsapiumsebiferum AT xuechen karrikinimprovesosmoticandsaltstresstoleranceviatheregulationoftheredoxhomeostasisintheoilplantsapiumsebiferum AT shengweihuang karrikinimprovesosmoticandsaltstresstoleranceviatheregulationoftheredoxhomeostasisintheoilplantsapiumsebiferum AT jinyanhou karrikinimprovesosmoticandsaltstresstoleranceviatheregulationoftheredoxhomeostasisintheoilplantsapiumsebiferum AT ruijulu karrikinimprovesosmoticandsaltstresstoleranceviatheregulationoftheredoxhomeostasisintheoilplantsapiumsebiferum AT chenghongliu karrikinimprovesosmoticandsaltstresstoleranceviatheregulationoftheredoxhomeostasisintheoilplantsapiumsebiferum AT junni karrikinimprovesosmoticandsaltstresstoleranceviatheregulationoftheredoxhomeostasisintheoilplantsapiumsebiferum AT lifangwu karrikinimprovesosmoticandsaltstresstoleranceviatheregulationoftheredoxhomeostasisintheoilplantsapiumsebiferum AT lifangwu karrikinimprovesosmoticandsaltstresstoleranceviatheregulationoftheredoxhomeostasisintheoilplantsapiumsebiferum |