Differentiation of Mesenchymal Stem Cell toward the Insulin-like Cells with Lentivirus Vector Mir-375
Background & Objective: Type1 diabetes is characterized by autoimmune destruction of pancreatic β cells, leading to reduced insulin secretion. Differentiation of mesenchymal stem cells (MSCs) into β-like cells offers new ways of diabetes treatment. MSCs can be insulated from the human umbilical...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Fasa University of Medical Sciences
2016-08-01
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| Series: | Journal of Advanced Biomedical Sciences |
| Subjects: | |
| Online Access: | http://jabs.fums.ac.ir/article-1-910-en.pdf |
| _version_ | 1797679647415074816 |
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| author | Hossein Sazegar Negar Azarpira Akbar Vahdati Mohamad hossein Karimi Masoud Soleimani |
| author_facet | Hossein Sazegar Negar Azarpira Akbar Vahdati Mohamad hossein Karimi Masoud Soleimani |
| author_sort | Hossein Sazegar |
| collection | DOAJ |
| description | Background & Objective: Type1 diabetes is characterized by autoimmune destruction of pancreatic β cells, leading to reduced insulin secretion. Differentiation of mesenchymal stem cells (MSCs) into β-like cells offers new ways of diabetes treatment. MSCs can be insulated from the human umbilical cord tissue and differentiate into insulin-producing cells.
Material & Methods: Human umbilical cord–derived stem cells (hUDSCs) were attained after birth, selected by plastic adhesion, and considered by flow cytometric analysis. For the differentiation induction, miR-375 was lentivirally over expressed in hUDSCs.
Results: Meaningful that microRNAs (miRNAs) are the key players in several stages of pancreatic Differentiation, we have presented a novel and cost-effective strategy in which the over-expression of miR-375 promotes the pancreatic differentiation in hUDSCs in the absence of any other stimulator. The major expressions of PDX1 and insulin were identified by quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR) (P <0.05).
Conclusion: In conclusion, the Morphological assessment and the expression analysis of islet marker genes demonstrated that hUDSCs are able to differentiate into insulin-producing cells by transduction with lentiviral vector miR-375 |
| first_indexed | 2024-03-11T23:17:51Z |
| format | Article |
| id | doaj.art-b4b07f7bd0e748f1814b387b95dfeba2 |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2783-1523 |
| language | English |
| last_indexed | 2024-03-11T23:17:51Z |
| publishDate | 2016-08-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Advanced Biomedical Sciences |
| spelling | doaj.art-b4b07f7bd0e748f1814b387b95dfeba22023-09-20T21:21:40ZengFasa University of Medical SciencesJournal of Advanced Biomedical Sciences2228-51052783-15232016-08-0162169177Differentiation of Mesenchymal Stem Cell toward the Insulin-like Cells with Lentivirus Vector Mir-375Hossein Sazegar0Negar Azarpira1Akbar Vahdati2Mohamad hossein Karimi3Masoud Soleimani4 Department Of Biology, Fars Science and Research Branch, Islamc Azad University, Fars, Iran Organ Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Department Of Biology, Shiraz branch, Islamc Azad University, Fars, Iran Organ Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Department of Hematology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran Background & Objective: Type1 diabetes is characterized by autoimmune destruction of pancreatic β cells, leading to reduced insulin secretion. Differentiation of mesenchymal stem cells (MSCs) into β-like cells offers new ways of diabetes treatment. MSCs can be insulated from the human umbilical cord tissue and differentiate into insulin-producing cells. Material & Methods: Human umbilical cord–derived stem cells (hUDSCs) were attained after birth, selected by plastic adhesion, and considered by flow cytometric analysis. For the differentiation induction, miR-375 was lentivirally over expressed in hUDSCs. Results: Meaningful that microRNAs (miRNAs) are the key players in several stages of pancreatic Differentiation, we have presented a novel and cost-effective strategy in which the over-expression of miR-375 promotes the pancreatic differentiation in hUDSCs in the absence of any other stimulator. The major expressions of PDX1 and insulin were identified by quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR) (P <0.05). Conclusion: In conclusion, the Morphological assessment and the expression analysis of islet marker genes demonstrated that hUDSCs are able to differentiate into insulin-producing cells by transduction with lentiviral vector miR-375http://jabs.fums.ac.ir/article-1-910-en.pdfdiabeteshudscsmir-375lentiviral vector |
| spellingShingle | Hossein Sazegar Negar Azarpira Akbar Vahdati Mohamad hossein Karimi Masoud Soleimani Differentiation of Mesenchymal Stem Cell toward the Insulin-like Cells with Lentivirus Vector Mir-375 Journal of Advanced Biomedical Sciences diabetes hudscs mir-375 lentiviral vector |
| title | Differentiation of Mesenchymal Stem Cell toward the Insulin-like Cells with Lentivirus Vector Mir-375 |
| title_full | Differentiation of Mesenchymal Stem Cell toward the Insulin-like Cells with Lentivirus Vector Mir-375 |
| title_fullStr | Differentiation of Mesenchymal Stem Cell toward the Insulin-like Cells with Lentivirus Vector Mir-375 |
| title_full_unstemmed | Differentiation of Mesenchymal Stem Cell toward the Insulin-like Cells with Lentivirus Vector Mir-375 |
| title_short | Differentiation of Mesenchymal Stem Cell toward the Insulin-like Cells with Lentivirus Vector Mir-375 |
| title_sort | differentiation of mesenchymal stem cell toward the insulin like cells with lentivirus vector mir 375 |
| topic | diabetes hudscs mir-375 lentiviral vector |
| url | http://jabs.fums.ac.ir/article-1-910-en.pdf |
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