Proanthocyanidins from <i>Vaccinium vitis-idaea</i> L. Leaves: Perspectives in Wound Healing and Designing for Topical Delivery
The compositions and health-beneficial properties of lingonberry leaves (<i>Vaccinium vitis-idaea</i> L.) are well established; however, their proanthocyanidins are still heavily underutilized. Optimizing their delivery systems is key to enabling their wider applications. The present stu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/19/2615 |
| _version_ | 1797477406896816128 |
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| author | Gabriele Vilkickyte Modestas Zilius Vilma Petrikaite Lina Raudone |
| author_facet | Gabriele Vilkickyte Modestas Zilius Vilma Petrikaite Lina Raudone |
| author_sort | Gabriele Vilkickyte |
| collection | DOAJ |
| description | The compositions and health-beneficial properties of lingonberry leaves (<i>Vaccinium vitis-idaea</i> L.) are well established; however, their proanthocyanidins are still heavily underutilized. Optimizing their delivery systems is key to enabling their wider applications. The present study investigates the phytochemical and ‘wound-healing’ properties of proanthocyanidin-rich fraction(s) (PRF) from lingonberry leaves as well as the development of optimal dermal film as a proanthocyanidin delivery system. The obtained PRF was subjected to HPLC-PDA and DMAC analyses to confirm the qualitative and quantitative profiles of different polymerization-degree proanthocyanidins. A ‘wound healing’ in vitro assay was performed to assess the ability of PRF to modulate the wound environment for better healing. Low concentrations of lingonberry proanthocyanidins were found to accelerate ‘wound‘ closures, while high levels inhibited human fibroblast migration. Fifteen dermal films containing PRF were prepared and evaluated based on their polymer (MC, HEC, PEG 400) compositions, and physical, mechanical, and biopharmaceutical properties using an experimental design. The composition containing 0.30 g of MC, 0.05 g of HEC, and 3.0 g of PEG 400 was selected as a promising formulation for PRF delivery and a potentially effective functional wound dressing material, supporting the need for further investigations. |
| first_indexed | 2024-03-09T21:17:12Z |
| format | Article |
| id | doaj.art-b4b2c0bc5f76492d9b24bb55df7b0e9c |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T21:17:12Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-b4b2c0bc5f76492d9b24bb55df7b0e9c2023-11-23T21:30:29ZengMDPI AGPlants2223-77472022-10-011119261510.3390/plants11192615Proanthocyanidins from <i>Vaccinium vitis-idaea</i> L. Leaves: Perspectives in Wound Healing and Designing for Topical DeliveryGabriele Vilkickyte0Modestas Zilius1Vilma Petrikaite2Lina Raudone3Laboratory of Biopharmaceutical Research, Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukileliu Av. 13, LT-50162 Kaunas, LithuaniaLaboratory of Pharmaceutical Sciences, Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukileliu Av. 13, LT-50162 Kaunas, LithuaniaLaboratory of Drug Targets Histopathology, Institute of Cardiology, Lithuanian University of Health Sciences, Sukileliu Av. 13, LT-50162 Kaunas, LithuaniaLaboratory of Biopharmaceutical Research, Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukileliu Av. 13, LT-50162 Kaunas, LithuaniaThe compositions and health-beneficial properties of lingonberry leaves (<i>Vaccinium vitis-idaea</i> L.) are well established; however, their proanthocyanidins are still heavily underutilized. Optimizing their delivery systems is key to enabling their wider applications. The present study investigates the phytochemical and ‘wound-healing’ properties of proanthocyanidin-rich fraction(s) (PRF) from lingonberry leaves as well as the development of optimal dermal film as a proanthocyanidin delivery system. The obtained PRF was subjected to HPLC-PDA and DMAC analyses to confirm the qualitative and quantitative profiles of different polymerization-degree proanthocyanidins. A ‘wound healing’ in vitro assay was performed to assess the ability of PRF to modulate the wound environment for better healing. Low concentrations of lingonberry proanthocyanidins were found to accelerate ‘wound‘ closures, while high levels inhibited human fibroblast migration. Fifteen dermal films containing PRF were prepared and evaluated based on their polymer (MC, HEC, PEG 400) compositions, and physical, mechanical, and biopharmaceutical properties using an experimental design. The composition containing 0.30 g of MC, 0.05 g of HEC, and 3.0 g of PEG 400 was selected as a promising formulation for PRF delivery and a potentially effective functional wound dressing material, supporting the need for further investigations.https://www.mdpi.com/2223-7747/11/19/2615lingonberryproanthocyanidinspolymeric filmsfilm propertieswound healing |
| spellingShingle | Gabriele Vilkickyte Modestas Zilius Vilma Petrikaite Lina Raudone Proanthocyanidins from <i>Vaccinium vitis-idaea</i> L. Leaves: Perspectives in Wound Healing and Designing for Topical Delivery Plants lingonberry proanthocyanidins polymeric films film properties wound healing |
| title | Proanthocyanidins from <i>Vaccinium vitis-idaea</i> L. Leaves: Perspectives in Wound Healing and Designing for Topical Delivery |
| title_full | Proanthocyanidins from <i>Vaccinium vitis-idaea</i> L. Leaves: Perspectives in Wound Healing and Designing for Topical Delivery |
| title_fullStr | Proanthocyanidins from <i>Vaccinium vitis-idaea</i> L. Leaves: Perspectives in Wound Healing and Designing for Topical Delivery |
| title_full_unstemmed | Proanthocyanidins from <i>Vaccinium vitis-idaea</i> L. Leaves: Perspectives in Wound Healing and Designing for Topical Delivery |
| title_short | Proanthocyanidins from <i>Vaccinium vitis-idaea</i> L. Leaves: Perspectives in Wound Healing and Designing for Topical Delivery |
| title_sort | proanthocyanidins from i vaccinium vitis idaea i l leaves perspectives in wound healing and designing for topical delivery |
| topic | lingonberry proanthocyanidins polymeric films film properties wound healing |
| url | https://www.mdpi.com/2223-7747/11/19/2615 |
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