Biochemical and Transcriptional Responses in Cold-Acclimated and Non-Acclimated Contrasting Camelina Biotypes under Freezing Stress
Cold-acclimated and non-acclimated contrasting Camelina (<i>Camelina sativa</i> L.) biotypes were investigated for changes in stress-associated biomarkers, including antioxidant enzyme activity, lipid peroxidation, protein, and proline content. In addition, a well-known freezing toleranc...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/22/3178 |
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| author | Jahad Soorni Seyed Kamal Kazemitabar Danial Kahrizi Ali Dehestani Nadali Bagheri Attila Kiss Péter Gergő Kovács István Papp Iman Mirmazloum |
| author_facet | Jahad Soorni Seyed Kamal Kazemitabar Danial Kahrizi Ali Dehestani Nadali Bagheri Attila Kiss Péter Gergő Kovács István Papp Iman Mirmazloum |
| author_sort | Jahad Soorni |
| collection | DOAJ |
| description | Cold-acclimated and non-acclimated contrasting Camelina (<i>Camelina sativa</i> L.) biotypes were investigated for changes in stress-associated biomarkers, including antioxidant enzyme activity, lipid peroxidation, protein, and proline content. In addition, a well-known freezing tolerance pathway participant known as C-repeat/DRE-binding factors (CBFs), an inducer of CBF expression (ICE1), and a cold-regulated (<i>COR6.6</i>) genes of the ICE-CBF-COR pathway were studied at the transcriptional level on the doubled-haploid (DH) lines. Freezing stress had significant effects on all studied parameters. The cold-acclimated DH34 (a freezing-tolerant line) showed an overall better performance under freezing stress than non-acclimated plants. The non-cold-acclimated DH08 (a frost-sensitive line) showed the highest electrolyte leakage after freezing stress. The highest activity of antioxidant enzymes (glutathione peroxidase, superoxide dismutase, and catalase) was also detected in non-acclimated plants, whereas the cold-acclimated plants showed lower enzyme activities upon stress treatment. Cold acclimation had a significantly positive effect on the total protein and proline content of stressed plants. The qRT-PCR analysis revealed significant differences in the expression and cold-inducibility of <i>CsCBF1-3</i>, <i>CsICE1</i>, and <i>CsCOR6.6</i> genes among the samples of different treatments. The highest expression of all CBF genes was recorded in the non-acclimated frost-tolerant biotype after freezing stress. Interestingly a significantly higher expression of <i>COR6.6</i> was detected in cold-acclimated samples of both frost-sensitive and -tolerant biotypes after freezing stress. The presented results provide more insights into freezing tolerance mechanisms in the Camelina plant from both a biochemical point of view and the expression of the associated genes. |
| first_indexed | 2024-03-09T18:04:14Z |
| format | Article |
| id | doaj.art-b4b3d432984847db83eee01837b7e827 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T18:04:14Z |
| publishDate | 2022-11-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-b4b3d432984847db83eee01837b7e8272023-11-24T09:40:56ZengMDPI AGPlants2223-77472022-11-011122317810.3390/plants11223178Biochemical and Transcriptional Responses in Cold-Acclimated and Non-Acclimated Contrasting Camelina Biotypes under Freezing StressJahad Soorni0Seyed Kamal Kazemitabar1Danial Kahrizi2Ali Dehestani3Nadali Bagheri4Attila Kiss5Péter Gergő Kovács6István Papp7Iman Mirmazloum8Department of Plant Breeding and Biotechnology, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari 68984, IranDepartment of Plant Breeding and Biotechnology, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari 68984, IranDepartment of Agronomy and Plant Breeding, Faculty of Agriculture, Razi University, Kermanshah 67144, IranGenetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University, Sari 68984, IranDepartment of Plant Breeding and Biotechnology, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari 68984, IranAgro-Food Science Techtransfer and Innovation Centre, Faculty for Agro-, Food- and Environmental Science, Debrecen University, H-4032 Debrecen, HungaryDepartment of Agronomy, Institute of Agronomy, Hungarian University of Agriculture and Life Sciences, 2100 Gödöllő, HungaryDepartment of Plant Physiology and Plant Ecology, Institute of Agronomy, Hungarian University of Agriculture and Life Sciences, 1118 Budapest, HungaryDepartment of Plant Physiology and Plant Ecology, Institute of Agronomy, Hungarian University of Agriculture and Life Sciences, 1118 Budapest, HungaryCold-acclimated and non-acclimated contrasting Camelina (<i>Camelina sativa</i> L.) biotypes were investigated for changes in stress-associated biomarkers, including antioxidant enzyme activity, lipid peroxidation, protein, and proline content. In addition, a well-known freezing tolerance pathway participant known as C-repeat/DRE-binding factors (CBFs), an inducer of CBF expression (ICE1), and a cold-regulated (<i>COR6.6</i>) genes of the ICE-CBF-COR pathway were studied at the transcriptional level on the doubled-haploid (DH) lines. Freezing stress had significant effects on all studied parameters. The cold-acclimated DH34 (a freezing-tolerant line) showed an overall better performance under freezing stress than non-acclimated plants. The non-cold-acclimated DH08 (a frost-sensitive line) showed the highest electrolyte leakage after freezing stress. The highest activity of antioxidant enzymes (glutathione peroxidase, superoxide dismutase, and catalase) was also detected in non-acclimated plants, whereas the cold-acclimated plants showed lower enzyme activities upon stress treatment. Cold acclimation had a significantly positive effect on the total protein and proline content of stressed plants. The qRT-PCR analysis revealed significant differences in the expression and cold-inducibility of <i>CsCBF1-3</i>, <i>CsICE1</i>, and <i>CsCOR6.6</i> genes among the samples of different treatments. The highest expression of all CBF genes was recorded in the non-acclimated frost-tolerant biotype after freezing stress. Interestingly a significantly higher expression of <i>COR6.6</i> was detected in cold-acclimated samples of both frost-sensitive and -tolerant biotypes after freezing stress. The presented results provide more insights into freezing tolerance mechanisms in the Camelina plant from both a biochemical point of view and the expression of the associated genes.https://www.mdpi.com/2223-7747/11/22/3178cold acclimationfreezing tolerance<i>C. sativa</i>electrolyte leakagegene expression |
| spellingShingle | Jahad Soorni Seyed Kamal Kazemitabar Danial Kahrizi Ali Dehestani Nadali Bagheri Attila Kiss Péter Gergő Kovács István Papp Iman Mirmazloum Biochemical and Transcriptional Responses in Cold-Acclimated and Non-Acclimated Contrasting Camelina Biotypes under Freezing Stress Plants cold acclimation freezing tolerance <i>C. sativa</i> electrolyte leakage gene expression |
| title | Biochemical and Transcriptional Responses in Cold-Acclimated and Non-Acclimated Contrasting Camelina Biotypes under Freezing Stress |
| title_full | Biochemical and Transcriptional Responses in Cold-Acclimated and Non-Acclimated Contrasting Camelina Biotypes under Freezing Stress |
| title_fullStr | Biochemical and Transcriptional Responses in Cold-Acclimated and Non-Acclimated Contrasting Camelina Biotypes under Freezing Stress |
| title_full_unstemmed | Biochemical and Transcriptional Responses in Cold-Acclimated and Non-Acclimated Contrasting Camelina Biotypes under Freezing Stress |
| title_short | Biochemical and Transcriptional Responses in Cold-Acclimated and Non-Acclimated Contrasting Camelina Biotypes under Freezing Stress |
| title_sort | biochemical and transcriptional responses in cold acclimated and non acclimated contrasting camelina biotypes under freezing stress |
| topic | cold acclimation freezing tolerance <i>C. sativa</i> electrolyte leakage gene expression |
| url | https://www.mdpi.com/2223-7747/11/22/3178 |
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