Expression of Some Metal Transporter ATPases in Brassica rapa Grown under Cadmium Stress
Cadmium (Cd) is one of the toxic heavy metals not essential for the growth and development of plants and other organisms. Brassica rapa accumulates several heavy metals in its leaves and is reportedly suitable for phytoremediation. In this study, Cd uptake and accumulation in the leaves of B. rapa e...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Turkish Science and Technology Publishing (TURSTEP)
2022-12-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
| Subjects: | |
| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/5640 |
| _version_ | 1797921116842360832 |
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| author | Merve Sahan Nuriye Meraklı Abdulrezzak Memon |
| author_facet | Merve Sahan Nuriye Meraklı Abdulrezzak Memon |
| author_sort | Merve Sahan |
| collection | DOAJ |
| description | Cadmium (Cd) is one of the toxic heavy metals not essential for the growth and development of plants and other organisms. Brassica rapa accumulates several heavy metals in its leaves and is reportedly suitable for phytoremediation. In this study, Cd uptake and accumulation in the leaves of B. rapa exposed to different cadmium levels (0, 5, 10, 20, 50, 100 μM CdSO4) were analyzed, as well as the effect of Cd stress on HMA2 and HMA4 genes expression were investigated. The qRT-PCR results showed that HMA2 and HMA4 play a vital role in cadmium uptake and translocation in B. rapa at low Cd levels (5-20 μM). However, exposure to high Cd levels (50 and 100 μM) significantly reduced the growth and biomass of B. rapa. Furthermore, high levels of Cd inhibit the expression of HMA2 and HMA4 genes in B. rapa. Our data show a multigenetic (co-acting of many transporter gene clusters) response in the signaling pathway associated with Cd accumulation and tolerance mechanism in B. rapa. |
| first_indexed | 2024-04-10T14:11:15Z |
| format | Article |
| id | doaj.art-b4b41bfa44b64eafa5abaac49800a365 |
| institution | Directory Open Access Journal |
| issn | 2148-127X |
| language | English |
| last_indexed | 2024-04-10T14:11:15Z |
| publishDate | 2022-12-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj.art-b4b41bfa44b64eafa5abaac49800a3652023-02-15T16:09:43ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2022-12-0110sp12685269010.24925/turjaf.v10isp1.2685-2690.56402723Expression of Some Metal Transporter ATPases in Brassica rapa Grown under Cadmium StressMerve Sahan0Nuriye Meraklı1Abdulrezzak Memon2Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Usak University, 64000 UsakDepartment of Molecular Biology and Genetics, Faculty of Arts and Sciences, Usak University, 64000 UsakDepartment of Molecular Biology and Genetics, Faculty of Arts and Sciences, Usak University, 64000 UsakCadmium (Cd) is one of the toxic heavy metals not essential for the growth and development of plants and other organisms. Brassica rapa accumulates several heavy metals in its leaves and is reportedly suitable for phytoremediation. In this study, Cd uptake and accumulation in the leaves of B. rapa exposed to different cadmium levels (0, 5, 10, 20, 50, 100 μM CdSO4) were analyzed, as well as the effect of Cd stress on HMA2 and HMA4 genes expression were investigated. The qRT-PCR results showed that HMA2 and HMA4 play a vital role in cadmium uptake and translocation in B. rapa at low Cd levels (5-20 μM). However, exposure to high Cd levels (50 and 100 μM) significantly reduced the growth and biomass of B. rapa. Furthermore, high levels of Cd inhibit the expression of HMA2 and HMA4 genes in B. rapa. Our data show a multigenetic (co-acting of many transporter gene clusters) response in the signaling pathway associated with Cd accumulation and tolerance mechanism in B. rapa.http://www.agrifoodscience.com/index.php/TURJAF/article/view/5640heavy metal atpaseshma2hma4cdbrassica rapa |
| spellingShingle | Merve Sahan Nuriye Meraklı Abdulrezzak Memon Expression of Some Metal Transporter ATPases in Brassica rapa Grown under Cadmium Stress Turkish Journal of Agriculture: Food Science and Technology heavy metal atpases hma2 hma4 cd brassica rapa |
| title | Expression of Some Metal Transporter ATPases in Brassica rapa Grown under Cadmium Stress |
| title_full | Expression of Some Metal Transporter ATPases in Brassica rapa Grown under Cadmium Stress |
| title_fullStr | Expression of Some Metal Transporter ATPases in Brassica rapa Grown under Cadmium Stress |
| title_full_unstemmed | Expression of Some Metal Transporter ATPases in Brassica rapa Grown under Cadmium Stress |
| title_short | Expression of Some Metal Transporter ATPases in Brassica rapa Grown under Cadmium Stress |
| title_sort | expression of some metal transporter atpases in brassica rapa grown under cadmium stress |
| topic | heavy metal atpases hma2 hma4 cd brassica rapa |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/5640 |
| work_keys_str_mv | AT mervesahan expressionofsomemetaltransporteratpasesinbrassicarapagrownundercadmiumstress AT nuriyemeraklı expressionofsomemetaltransporteratpasesinbrassicarapagrownundercadmiumstress AT abdulrezzakmemon expressionofsomemetaltransporteratpasesinbrassicarapagrownundercadmiumstress |