Molecular characterization of circulating strains of the peste-des-petitis-ruminants virus in Sulaimani province, Iraq
Peste des petits ruminants (PPR) are caused by a Morbillivirus that belongs to the family Paramyxoviridae. Peste des petits ruminants are an acute, highly contagious, and deadly disease that mainly affects goats and sheep, with subclinical infection occurring in cattle. Peste des petits ruminants oc...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2023-07-01
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| Series: | Iraqi Journal of Veterinary Sciences |
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| Online Access: | https://www.vetmedmosul.com/article_178796_0982e13b9d78903b7310054097500764.pdf |
| Summary: | Peste des petits ruminants (PPR) are caused by a Morbillivirus that belongs to the family Paramyxoviridae. Peste des petits ruminants are an acute, highly contagious, and deadly disease that mainly affects goats and sheep, with subclinical infection occurring in cattle. Peste des petits ruminants occur in many countries, with morbidity and fatality rates as high as 90 percent. Peste des petits ruminants’ outbreaks occurred annually in Sulaymaniyah; this study aims to determine genetic variation and the global relationship of circulating strains in the area. RT-PCR was primarily used to identify the virus from mouth epithelial samples. The direct sequencing and subsequent analysis of amplified PCR products for the (N and F) genes revealed the presence of PPRV lineage IV in the study areas. Moreover, phylogenetic analysis revealed N gene (OL702851/PPR/H/SUL/sheep, OL702852/PPR/H/SUL2/Goat) cluster with (china-KP319027.1) strain with identity 98%, while the F gene (PPR/H/SUL3/Sheep, PPR/H/SUL4/Goat) has a cluster with (Turkey-MG744218 and Saudi Arabia-MK922470.1) strains with identities 97 and 98% respectably. Furthermore, phylogenetic tree field virus isolates heterologous with the current vaccine strain (Nigeria 75/1) were used for the mass vaccination program, and the divergence rate was 10%-13%. These variations between vaccines and field isolates could compromise the antibody responses induced by the vaccines in the hosts. |
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| ISSN: | 1607-3894 2071-1255 |