Metallo-Liposomes Derived from the [Ru(bpy)<sub>3</sub>]<sup>2+</sup> Complex as Nanocarriers of Therapeutic Agents
The obtaining of nanocarriers of gene material and small drugs is still an interesting research line. Side-effects produced by the toxicity of several pharmaceutics, the high concentrations needed to get therapeutic effects, or their excessive use by patients have motivated the search for new nanost...
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MDPI AG
2021-04-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/9/5/90 |
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| author | Maria Luisa Moyá Francisco José Ostos Izamar Moreno Diandra García Paula Moreno-Gordillo Ivan V. Rosado Pilar López-Cornejo José Antonio Lebrón Manuel López-López |
| author_facet | Maria Luisa Moyá Francisco José Ostos Izamar Moreno Diandra García Paula Moreno-Gordillo Ivan V. Rosado Pilar López-Cornejo José Antonio Lebrón Manuel López-López |
| author_sort | Maria Luisa Moyá |
| collection | DOAJ |
| description | The obtaining of nanocarriers of gene material and small drugs is still an interesting research line. Side-effects produced by the toxicity of several pharmaceutics, the high concentrations needed to get therapeutic effects, or their excessive use by patients have motivated the search for new nanostructures. For these reasons, cationic metallo-liposomes composed by phosphatidylcholine (PC), cholesterol (CHO) and RuC1C19 (a surfactant derived from the metallic complex [Ru(bpy)<sub>3</sub>]<sup>2+</sup>) were prepared and characterized by using diverse techniques (zeta potential, dynamic light scattering and electronic transmission microscopy –TEM-). Unimodal or bimodal populations of spherical aggregates with small sizes were obtained depending on the composition of the liposomes. The presence of cholesterol favored the formation of small aggregates. ct-DNA was condensed in the presence of the liposomes investigated. In-vitro assays demonstrated the ability of these nanoaggregates to internalize into different cell lines. A positive gene transfection into human bone osteosarcoma epithelial cells (U2OS) was also observed. The RuC1C19 surfactant was used as sensor to quantify the binding of DNA to the liposomes. Doxorubicin was encapsulated into the metallo-liposomes, demonstrating their ability to be also used as nanocarriers of drugs. A relationship between then encapsulation percentage of the antibiotic and the composition of the aggregates has been established. |
| first_indexed | 2024-03-10T11:58:14Z |
| format | Article |
| id | doaj.art-b4b827e5bbda4f949b9c018372c12cff |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-10T11:58:14Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-b4b827e5bbda4f949b9c018372c12cff2023-11-21T17:07:32ZengMDPI AGChemosensors2227-90402021-04-01959010.3390/chemosensors9050090Metallo-Liposomes Derived from the [Ru(bpy)<sub>3</sub>]<sup>2+</sup> Complex as Nanocarriers of Therapeutic AgentsMaria Luisa Moyá0Francisco José Ostos1Izamar Moreno2Diandra García3Paula Moreno-Gordillo4Ivan V. Rosado5Pilar López-Cornejo6José Antonio Lebrón7Manuel López-López8Department of Physical Chemistry, Faculty of Chemistry, University of Seville, C/Professor García González 1, 41012 Seville, SpainDepartment of Physical Chemistry, Faculty of Chemistry, University of Seville, C/Professor García González 1, 41012 Seville, SpainDepartment of Physical Chemistry, Faculty of Chemistry, University of Seville, C/Professor García González 1, 41012 Seville, SpainDepartment of Physical Chemistry, Faculty of Chemistry, University of Seville, C/Professor García González 1, 41012 Seville, SpainInstitute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocio/CSIC/University of Seville, Avda. Manuel Siurot s/n, 41013 Seville, SpainInstitute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocio/CSIC/University of Seville, Avda. Manuel Siurot s/n, 41013 Seville, SpainDepartment of Physical Chemistry, Faculty of Chemistry, University of Seville, C/Professor García González 1, 41012 Seville, SpainDepartment of Physical Chemistry, Faculty of Chemistry, University of Seville, C/Professor García González 1, 41012 Seville, SpainDepartment of Chemical Engineering, Physical Chemistry and Materials Science, Faculty of Experimental Sciences, Campus de El Carmen, Avda. de las Fuerzas Armadas s/n, 21071 Huelva, SpainThe obtaining of nanocarriers of gene material and small drugs is still an interesting research line. Side-effects produced by the toxicity of several pharmaceutics, the high concentrations needed to get therapeutic effects, or their excessive use by patients have motivated the search for new nanostructures. For these reasons, cationic metallo-liposomes composed by phosphatidylcholine (PC), cholesterol (CHO) and RuC1C19 (a surfactant derived from the metallic complex [Ru(bpy)<sub>3</sub>]<sup>2+</sup>) were prepared and characterized by using diverse techniques (zeta potential, dynamic light scattering and electronic transmission microscopy –TEM-). Unimodal or bimodal populations of spherical aggregates with small sizes were obtained depending on the composition of the liposomes. The presence of cholesterol favored the formation of small aggregates. ct-DNA was condensed in the presence of the liposomes investigated. In-vitro assays demonstrated the ability of these nanoaggregates to internalize into different cell lines. A positive gene transfection into human bone osteosarcoma epithelial cells (U2OS) was also observed. The RuC1C19 surfactant was used as sensor to quantify the binding of DNA to the liposomes. Doxorubicin was encapsulated into the metallo-liposomes, demonstrating their ability to be also used as nanocarriers of drugs. A relationship between then encapsulation percentage of the antibiotic and the composition of the aggregates has been established.https://www.mdpi.com/2227-9040/9/5/90metallo-liposomenanocarriergene therapyDNAdoxorubicinTEM |
| spellingShingle | Maria Luisa Moyá Francisco José Ostos Izamar Moreno Diandra García Paula Moreno-Gordillo Ivan V. Rosado Pilar López-Cornejo José Antonio Lebrón Manuel López-López Metallo-Liposomes Derived from the [Ru(bpy)<sub>3</sub>]<sup>2+</sup> Complex as Nanocarriers of Therapeutic Agents Chemosensors metallo-liposome nanocarrier gene therapy DNA doxorubicin TEM |
| title | Metallo-Liposomes Derived from the [Ru(bpy)<sub>3</sub>]<sup>2+</sup> Complex as Nanocarriers of Therapeutic Agents |
| title_full | Metallo-Liposomes Derived from the [Ru(bpy)<sub>3</sub>]<sup>2+</sup> Complex as Nanocarriers of Therapeutic Agents |
| title_fullStr | Metallo-Liposomes Derived from the [Ru(bpy)<sub>3</sub>]<sup>2+</sup> Complex as Nanocarriers of Therapeutic Agents |
| title_full_unstemmed | Metallo-Liposomes Derived from the [Ru(bpy)<sub>3</sub>]<sup>2+</sup> Complex as Nanocarriers of Therapeutic Agents |
| title_short | Metallo-Liposomes Derived from the [Ru(bpy)<sub>3</sub>]<sup>2+</sup> Complex as Nanocarriers of Therapeutic Agents |
| title_sort | metallo liposomes derived from the ru bpy sub 3 sub sup 2 sup complex as nanocarriers of therapeutic agents |
| topic | metallo-liposome nanocarrier gene therapy DNA doxorubicin TEM |
| url | https://www.mdpi.com/2227-9040/9/5/90 |
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