Toxicological assessment of pristine and degraded forms of graphene functionalized with MnOx nanoparticles using human in vitro models representing different exposure routes
Abstract The development of novel advanced nanomaterials (NMs) with outstanding characteristics for their use in distinct applications needs to be accompanied by the generation of knowledge on their potential toxicological impact, in particular, that derived from different occupational risk exposure...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-07-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-38993-y |
| _version_ | 1827895204865114112 |
|---|---|
| author | Natalia Fernández-Pampín Juan José González Plaza Alejandra García-Gómez Elisa Peña Sebastiano Garroni Matteo Poddighe Carlos Rumbo Rocío Barros Sonia Martel-Martín Santiago Aparicio Juan Antonio Tamayo-Ramos |
| author_facet | Natalia Fernández-Pampín Juan José González Plaza Alejandra García-Gómez Elisa Peña Sebastiano Garroni Matteo Poddighe Carlos Rumbo Rocío Barros Sonia Martel-Martín Santiago Aparicio Juan Antonio Tamayo-Ramos |
| author_sort | Natalia Fernández-Pampín |
| collection | DOAJ |
| description | Abstract The development of novel advanced nanomaterials (NMs) with outstanding characteristics for their use in distinct applications needs to be accompanied by the generation of knowledge on their potential toxicological impact, in particular, that derived from different occupational risk exposure routes, such as inhalation, ingestion, and skin contact. The harmful effects of novel graphene-metal oxide composites on human health are not well understood, many toxicological properties have not been investigated yet. The present study has evaluated several toxicological effects associated with graphene decorated with manganese oxide nanoparticles (GNA15), in a comparative assessment with those induced by simple graphene (G2), on human models representing inhalation (A549 cell line), ingestion (HT29 cell line) and dermal routes (3D reconstructed skin). Pristine and degraded forms of these NMs were included in the study, showing to have different physicochemical and toxicological properties. The degraded version of GNA15 (GNA15d) and G2 (G2d) exhibited clear structural differences with their pristine counterparts, as well as a higher release of metal ions. The viability of respiratory and gastrointestinal models was reduced in a dose-dependent manner in the presence of both GNA15 and G2 pristine and degraded forms. Besides this, all NMs induced the production of reactive oxygen species (ROS) in both models. However, the degraded forms showed to induce a higher cytotoxicity effect. In addition, we found that none of the materials produced irritant effects on 3D reconstructed skin when present in aqueous suspensions. These results provide novel insights into the potentially harmful effects of novel multicomponent NMs in a comprehensive manner. Furthermore, the integrity of the NMs can play a role in their toxicity, which can vary depending on their composition and the exposure route. |
| first_indexed | 2024-03-12T22:17:10Z |
| format | Article |
| id | doaj.art-b4b83c7aa1c54e93b3926a723120590b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-12T22:17:10Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b4b83c7aa1c54e93b3926a723120590b2023-07-23T11:14:43ZengNature PortfolioScientific Reports2045-23222023-07-0113111410.1038/s41598-023-38993-yToxicological assessment of pristine and degraded forms of graphene functionalized with MnOx nanoparticles using human in vitro models representing different exposure routesNatalia Fernández-Pampín0Juan José González Plaza1Alejandra García-Gómez2Elisa Peña3Sebastiano Garroni4Matteo Poddighe5Carlos Rumbo6Rocío Barros7Sonia Martel-Martín8Santiago Aparicio9Juan Antonio Tamayo-Ramos10International Research Centre in Critical Raw Materials-ICCRAM, University of BurgosInternational Research Centre in Critical Raw Materials-ICCRAM, University of BurgosGnanomatGnanomatDepartment of Chemical, Physics, Mathematics and Natural Science, University of SassariLaboratory of Materials Science and Nanotechnology (LMNT), Department of Chemical, Physics, Mathematics and Natural Science, CR-INSTM, University of SassariInternational Research Centre in Critical Raw Materials-ICCRAM, University of BurgosInternational Research Centre in Critical Raw Materials-ICCRAM, University of BurgosInternational Research Centre in Critical Raw Materials-ICCRAM, University of BurgosInternational Research Centre in Critical Raw Materials-ICCRAM, University of BurgosInternational Research Centre in Critical Raw Materials-ICCRAM, University of BurgosAbstract The development of novel advanced nanomaterials (NMs) with outstanding characteristics for their use in distinct applications needs to be accompanied by the generation of knowledge on their potential toxicological impact, in particular, that derived from different occupational risk exposure routes, such as inhalation, ingestion, and skin contact. The harmful effects of novel graphene-metal oxide composites on human health are not well understood, many toxicological properties have not been investigated yet. The present study has evaluated several toxicological effects associated with graphene decorated with manganese oxide nanoparticles (GNA15), in a comparative assessment with those induced by simple graphene (G2), on human models representing inhalation (A549 cell line), ingestion (HT29 cell line) and dermal routes (3D reconstructed skin). Pristine and degraded forms of these NMs were included in the study, showing to have different physicochemical and toxicological properties. The degraded version of GNA15 (GNA15d) and G2 (G2d) exhibited clear structural differences with their pristine counterparts, as well as a higher release of metal ions. The viability of respiratory and gastrointestinal models was reduced in a dose-dependent manner in the presence of both GNA15 and G2 pristine and degraded forms. Besides this, all NMs induced the production of reactive oxygen species (ROS) in both models. However, the degraded forms showed to induce a higher cytotoxicity effect. In addition, we found that none of the materials produced irritant effects on 3D reconstructed skin when present in aqueous suspensions. These results provide novel insights into the potentially harmful effects of novel multicomponent NMs in a comprehensive manner. Furthermore, the integrity of the NMs can play a role in their toxicity, which can vary depending on their composition and the exposure route.https://doi.org/10.1038/s41598-023-38993-y |
| spellingShingle | Natalia Fernández-Pampín Juan José González Plaza Alejandra García-Gómez Elisa Peña Sebastiano Garroni Matteo Poddighe Carlos Rumbo Rocío Barros Sonia Martel-Martín Santiago Aparicio Juan Antonio Tamayo-Ramos Toxicological assessment of pristine and degraded forms of graphene functionalized with MnOx nanoparticles using human in vitro models representing different exposure routes Scientific Reports |
| title | Toxicological assessment of pristine and degraded forms of graphene functionalized with MnOx nanoparticles using human in vitro models representing different exposure routes |
| title_full | Toxicological assessment of pristine and degraded forms of graphene functionalized with MnOx nanoparticles using human in vitro models representing different exposure routes |
| title_fullStr | Toxicological assessment of pristine and degraded forms of graphene functionalized with MnOx nanoparticles using human in vitro models representing different exposure routes |
| title_full_unstemmed | Toxicological assessment of pristine and degraded forms of graphene functionalized with MnOx nanoparticles using human in vitro models representing different exposure routes |
| title_short | Toxicological assessment of pristine and degraded forms of graphene functionalized with MnOx nanoparticles using human in vitro models representing different exposure routes |
| title_sort | toxicological assessment of pristine and degraded forms of graphene functionalized with mnox nanoparticles using human in vitro models representing different exposure routes |
| url | https://doi.org/10.1038/s41598-023-38993-y |
| work_keys_str_mv | AT nataliafernandezpampin toxicologicalassessmentofpristineanddegradedformsofgraphenefunctionalizedwithmnoxnanoparticlesusinghumaninvitromodelsrepresentingdifferentexposureroutes AT juanjosegonzalezplaza toxicologicalassessmentofpristineanddegradedformsofgraphenefunctionalizedwithmnoxnanoparticlesusinghumaninvitromodelsrepresentingdifferentexposureroutes AT alejandragarciagomez toxicologicalassessmentofpristineanddegradedformsofgraphenefunctionalizedwithmnoxnanoparticlesusinghumaninvitromodelsrepresentingdifferentexposureroutes AT elisapena toxicologicalassessmentofpristineanddegradedformsofgraphenefunctionalizedwithmnoxnanoparticlesusinghumaninvitromodelsrepresentingdifferentexposureroutes AT sebastianogarroni toxicologicalassessmentofpristineanddegradedformsofgraphenefunctionalizedwithmnoxnanoparticlesusinghumaninvitromodelsrepresentingdifferentexposureroutes AT matteopoddighe toxicologicalassessmentofpristineanddegradedformsofgraphenefunctionalizedwithmnoxnanoparticlesusinghumaninvitromodelsrepresentingdifferentexposureroutes AT carlosrumbo toxicologicalassessmentofpristineanddegradedformsofgraphenefunctionalizedwithmnoxnanoparticlesusinghumaninvitromodelsrepresentingdifferentexposureroutes AT rociobarros toxicologicalassessmentofpristineanddegradedformsofgraphenefunctionalizedwithmnoxnanoparticlesusinghumaninvitromodelsrepresentingdifferentexposureroutes AT soniamartelmartin toxicologicalassessmentofpristineanddegradedformsofgraphenefunctionalizedwithmnoxnanoparticlesusinghumaninvitromodelsrepresentingdifferentexposureroutes AT santiagoaparicio toxicologicalassessmentofpristineanddegradedformsofgraphenefunctionalizedwithmnoxnanoparticlesusinghumaninvitromodelsrepresentingdifferentexposureroutes AT juanantoniotamayoramos toxicologicalassessmentofpristineanddegradedformsofgraphenefunctionalizedwithmnoxnanoparticlesusinghumaninvitromodelsrepresentingdifferentexposureroutes |