Silver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virus
Rapid non-laboratory screening of plants for pathogenic viruses crucially influences crop yields in modern agricultural technologies. The aim of this study was to develop a highly-sensitive lateral flow immunoassay (LFIA) for rapid detection of potato leafroll virus (PLRV), an infectious agent of on...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2017.1401044 |
| _version_ | 1818694794642194432 |
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| author | Vasily G. Panferov Irina V. Safenkova Nadezhda A. Byzova Yuri A. Varitsev Anatoly V. Zherdev Boris B. Dzantiev |
| author_facet | Vasily G. Panferov Irina V. Safenkova Nadezhda A. Byzova Yuri A. Varitsev Anatoly V. Zherdev Boris B. Dzantiev |
| author_sort | Vasily G. Panferov |
| collection | DOAJ |
| description | Rapid non-laboratory screening of plants for pathogenic viruses crucially influences crop yields in modern agricultural technologies. The aim of this study was to develop a highly-sensitive lateral flow immunoassay (LFIA) for rapid detection of potato leafroll virus (PLRV), an infectious agent of one of the most widespread potato diseases. The proposed LFIA combines the formation of sandwich immune complexes with gold nanoparticles (GNP) as labels and silver enhancement. The enhancement stage was realized using mixture of silver lactate and hydroquinone and subsequent addition of chloride-containing buffer to stop silver reduction. LFIA with silver enhancement was 15 times more sensitive (detection limit 0.2 ng/mL; 15 min) compared with conventional LFIA (detection limit 3 ng/mL; 10 min). The enhanced LFIA detected PLRV in leaves’ extracts of infected potato in dilutions higher than enzyme-linked immunosorbent assay. |
| first_indexed | 2024-12-17T13:35:15Z |
| format | Article |
| id | doaj.art-b4b87754839243bbb1ffc4bbda13f71e |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-17T13:35:15Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-b4b87754839243bbb1ffc4bbda13f71e2022-12-21T21:46:28ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432018-01-0129144545710.1080/09540105.2017.14010441401044Silver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virusVasily G. Panferov0Irina V. Safenkova1Nadezhda A. Byzova2Yuri A. Varitsev3Anatoly V. Zherdev4Boris B. Dzantiev5A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of SciencesA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of SciencesA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of SciencesA.G. Lorch All-Russian Potato Research InstituteA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of SciencesA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of SciencesRapid non-laboratory screening of plants for pathogenic viruses crucially influences crop yields in modern agricultural technologies. The aim of this study was to develop a highly-sensitive lateral flow immunoassay (LFIA) for rapid detection of potato leafroll virus (PLRV), an infectious agent of one of the most widespread potato diseases. The proposed LFIA combines the formation of sandwich immune complexes with gold nanoparticles (GNP) as labels and silver enhancement. The enhancement stage was realized using mixture of silver lactate and hydroquinone and subsequent addition of chloride-containing buffer to stop silver reduction. LFIA with silver enhancement was 15 times more sensitive (detection limit 0.2 ng/mL; 15 min) compared with conventional LFIA (detection limit 3 ng/mL; 10 min). The enhanced LFIA detected PLRV in leaves’ extracts of infected potato in dilutions higher than enzyme-linked immunosorbent assay.http://dx.doi.org/10.1080/09540105.2017.1401044gold nanoparticleslateral flow immunoassaysilver enhancementpotato leafroll viruspotato infections |
| spellingShingle | Vasily G. Panferov Irina V. Safenkova Nadezhda A. Byzova Yuri A. Varitsev Anatoly V. Zherdev Boris B. Dzantiev Silver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virus Food and Agricultural Immunology gold nanoparticles lateral flow immunoassay silver enhancement potato leafroll virus potato infections |
| title | Silver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virus |
| title_full | Silver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virus |
| title_fullStr | Silver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virus |
| title_full_unstemmed | Silver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virus |
| title_short | Silver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virus |
| title_sort | silver enhanced lateral flow immunoassay for highly sensitive detection of potato leafroll virus |
| topic | gold nanoparticles lateral flow immunoassay silver enhancement potato leafroll virus potato infections |
| url | http://dx.doi.org/10.1080/09540105.2017.1401044 |
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