IL-23/IL-27 Ratio in Peripheral Blood of Patients with Breast Cancer
Background: Interleukin (IL)-23 and IL-27 are two IL-12-related cytokines which their function may dramatically influence the inflammatory response to tumor development. IL-12 and IL-27 seem to have antagonistic roles with IL-23 in tumor site. In this study, IL-23 and IL-27 mRNA expressions were ana...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2014-07-01
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| Series: | Iranian Journal of Medical Sciences |
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| Online Access: | http://ijms.sums.ac.ir/index.php/IJMS/article/view/949 |
| _version_ | 1818178821427298304 |
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| author | Ali Khodadadi Mahboobeh Razmkhah Ali-Reza Eskandari Ahmad Hosseini Mojtaba Habibagahi bbas Ghaderi Mansooreh Jaberipour |
| author_facet | Ali Khodadadi Mahboobeh Razmkhah Ali-Reza Eskandari Ahmad Hosseini Mojtaba Habibagahi bbas Ghaderi Mansooreh Jaberipour |
| author_sort | Ali Khodadadi |
| collection | DOAJ |
| description | Background: Interleukin (IL)-23 and IL-27 are two IL-12-related cytokines which their function may dramatically influence the inflammatory response to tumor development. IL-12 and IL-27 seem to have antagonistic roles with IL-23 in tumor site. In this study, IL-23 and IL-27 mRNA expressions were analyzed in peripheral blood of patients with breast cancer and healthy volunteers using quantitative real-time PCR.
Methods: Peripheral blood samples were collected from 50 women with breast cancer and 50 healthy ones. The total RNA was extracted from peripheral blood after lysis with ammonium chloride and TRizol reagent and the cDNA was synthesized. The expression of IL-23 and IL-27 gene transcripts was determined with real-time polymerase chain reaction (qRT-PCR) using Syber Green PCR Master Mix.
Results: It is found that IL-23 and IL-27 transcripts had significantly higher expression in peripheral blood of patients compared with the healthy controls. The ratio of IL-23 transcript expression to IL-27 was 3.4 fold lower in the studied patients compared with the normal individuals.
Conclusion: It is concluded that the over expression of IL-23 and IL-27 gene transcript in peripheral blood of breast cancer patients may be an immune response against tumor development and the inflammatory response plays a critical role in tumor development via up regulating the corresponding cytokines. However, the IL-23/IL-27 ratio may play an important role in cytokine-based immunotherapy against cancer. Further research should be carried out to assess these cytokines in a larger sample size. |
| first_indexed | 2024-12-11T20:54:04Z |
| format | Article |
| id | doaj.art-b4ba8080719d4c51af012ea13f0f68b3 |
| institution | Directory Open Access Journal |
| issn | 0253-0716 1735-3688 |
| language | English |
| last_indexed | 2024-12-11T20:54:04Z |
| publishDate | 2014-07-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Medical Sciences |
| spelling | doaj.art-b4ba8080719d4c51af012ea13f0f68b32022-12-22T00:51:10ZengShiraz University of Medical SciencesIranian Journal of Medical Sciences0253-07161735-36882014-07-01394350356IL-23/IL-27 Ratio in Peripheral Blood of Patients with Breast CancerAli Khodadadi0Mahboobeh Razmkhah1Ali-Reza Eskandari2Ahmad Hosseini3Mojtaba Habibagahi4bbas Ghaderi5Mansooreh Jaberipour6Department of Immunology, Ahvaz Jondishapur University of Medical Sciences, Ahvaz, Iran;Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;Department of Immunology, Ahvaz Jondishapur University of Medical Sciences, Ahvaz, Iran;Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;Background: Interleukin (IL)-23 and IL-27 are two IL-12-related cytokines which their function may dramatically influence the inflammatory response to tumor development. IL-12 and IL-27 seem to have antagonistic roles with IL-23 in tumor site. In this study, IL-23 and IL-27 mRNA expressions were analyzed in peripheral blood of patients with breast cancer and healthy volunteers using quantitative real-time PCR. Methods: Peripheral blood samples were collected from 50 women with breast cancer and 50 healthy ones. The total RNA was extracted from peripheral blood after lysis with ammonium chloride and TRizol reagent and the cDNA was synthesized. The expression of IL-23 and IL-27 gene transcripts was determined with real-time polymerase chain reaction (qRT-PCR) using Syber Green PCR Master Mix. Results: It is found that IL-23 and IL-27 transcripts had significantly higher expression in peripheral blood of patients compared with the healthy controls. The ratio of IL-23 transcript expression to IL-27 was 3.4 fold lower in the studied patients compared with the normal individuals. Conclusion: It is concluded that the over expression of IL-23 and IL-27 gene transcript in peripheral blood of breast cancer patients may be an immune response against tumor development and the inflammatory response plays a critical role in tumor development via up regulating the corresponding cytokines. However, the IL-23/IL-27 ratio may play an important role in cytokine-based immunotherapy against cancer. Further research should be carried out to assess these cytokines in a larger sample size.http://ijms.sums.ac.ir/index.php/IJMS/article/view/949NeoplasmsBreast NeoplasmInterleukin-23Interleukin-27 |
| spellingShingle | Ali Khodadadi Mahboobeh Razmkhah Ali-Reza Eskandari Ahmad Hosseini Mojtaba Habibagahi bbas Ghaderi Mansooreh Jaberipour IL-23/IL-27 Ratio in Peripheral Blood of Patients with Breast Cancer Iranian Journal of Medical Sciences Neoplasms Breast Neoplasm Interleukin-23 Interleukin-27 |
| title | IL-23/IL-27 Ratio in Peripheral Blood of Patients with Breast Cancer |
| title_full | IL-23/IL-27 Ratio in Peripheral Blood of Patients with Breast Cancer |
| title_fullStr | IL-23/IL-27 Ratio in Peripheral Blood of Patients with Breast Cancer |
| title_full_unstemmed | IL-23/IL-27 Ratio in Peripheral Blood of Patients with Breast Cancer |
| title_short | IL-23/IL-27 Ratio in Peripheral Blood of Patients with Breast Cancer |
| title_sort | il 23 il 27 ratio in peripheral blood of patients with breast cancer |
| topic | Neoplasms Breast Neoplasm Interleukin-23 Interleukin-27 |
| url | http://ijms.sums.ac.ir/index.php/IJMS/article/view/949 |
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