The tardigrade Dsup protein enhances radioresistance in Drosophila melanogaster and acts as an unspecific repressor of transcription
Summary: The tardigrade-unique damage suppressor protein (Dsup) can protect DNA from ionizing radiation and reactive oxygen species (ROS). In this study, we generated Dsup-expressing lines of Drosophila melanogaster and demonstrated that Dsup increased the survival rate after γ-ray irradiation and h...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-07-01
|
| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223010751 |
| _version_ | 1797774566394691584 |
|---|---|
| author | Mikhail Zarubin Talyana Azorskaya Olga Kuldoshina Sergey Alekseev Semen Mitrofanov Elena Kravchenko |
| author_facet | Mikhail Zarubin Talyana Azorskaya Olga Kuldoshina Sergey Alekseev Semen Mitrofanov Elena Kravchenko |
| author_sort | Mikhail Zarubin |
| collection | DOAJ |
| description | Summary: The tardigrade-unique damage suppressor protein (Dsup) can protect DNA from ionizing radiation and reactive oxygen species (ROS). In this study, we generated Dsup-expressing lines of Drosophila melanogaster and demonstrated that Dsup increased the survival rate after γ-ray irradiation and hydrogen peroxide treatment in flies too, but reduced the level of their locomotor activity. The transcriptome analyses of Dsup-expressing lines revealed a significant number of DEGs, >99% of which were down-regulated. Moreover, Dsup could bind RNA. These findings suggest that Dsup can act not only as a DNA protector but also as a non-specific transcriptional repressor and RNA-binding protein, that may lead to disturbance of a number of biological processes in D. melanogaster. The obtained data demonstrate features of the Dsup protein action in non-tardigrade organisms and can be used to understand the impact of other unspecific DNA/RNA-binding proteins on ROS and radiation resistance, gene expression, and epigenetic processes. |
| first_indexed | 2024-03-12T22:22:56Z |
| format | Article |
| id | doaj.art-b4bea942dc394ab08b01fb52cad54071 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-12T22:22:56Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-b4bea942dc394ab08b01fb52cad540712023-07-23T04:55:04ZengElsevieriScience2589-00422023-07-01267106998The tardigrade Dsup protein enhances radioresistance in Drosophila melanogaster and acts as an unspecific repressor of transcriptionMikhail Zarubin0Talyana Azorskaya1Olga Kuldoshina2Sergey Alekseev3Semen Mitrofanov4Elena Kravchenko5Dzhelepov Laboratory of Nuclear Problems, International Intergovernmental Organization Joint Institute for Nuclear Research, Dubna 141980, RussiaDzhelepov Laboratory of Nuclear Problems, International Intergovernmental Organization Joint Institute for Nuclear Research, Dubna 141980, RussiaDzhelepov Laboratory of Nuclear Problems, International Intergovernmental Organization Joint Institute for Nuclear Research, Dubna 141980, RussiaFlerov Laboratory of Nuclear Reactions, International Intergovernmental Organization Joint Institute for Nuclear Research, Dubna 141980, RussiaFlerov Laboratory of Nuclear Reactions, International Intergovernmental Organization Joint Institute for Nuclear Research, Dubna 141980, RussiaDzhelepov Laboratory of Nuclear Problems, International Intergovernmental Organization Joint Institute for Nuclear Research, Dubna 141980, Russia; Corresponding authorSummary: The tardigrade-unique damage suppressor protein (Dsup) can protect DNA from ionizing radiation and reactive oxygen species (ROS). In this study, we generated Dsup-expressing lines of Drosophila melanogaster and demonstrated that Dsup increased the survival rate after γ-ray irradiation and hydrogen peroxide treatment in flies too, but reduced the level of their locomotor activity. The transcriptome analyses of Dsup-expressing lines revealed a significant number of DEGs, >99% of which were down-regulated. Moreover, Dsup could bind RNA. These findings suggest that Dsup can act not only as a DNA protector but also as a non-specific transcriptional repressor and RNA-binding protein, that may lead to disturbance of a number of biological processes in D. melanogaster. The obtained data demonstrate features of the Dsup protein action in non-tardigrade organisms and can be used to understand the impact of other unspecific DNA/RNA-binding proteins on ROS and radiation resistance, gene expression, and epigenetic processes.http://www.sciencedirect.com/science/article/pii/S2589004223010751Molecular biologyCell biologyTranscriptomics |
| spellingShingle | Mikhail Zarubin Talyana Azorskaya Olga Kuldoshina Sergey Alekseev Semen Mitrofanov Elena Kravchenko The tardigrade Dsup protein enhances radioresistance in Drosophila melanogaster and acts as an unspecific repressor of transcription iScience Molecular biology Cell biology Transcriptomics |
| title | The tardigrade Dsup protein enhances radioresistance in Drosophila melanogaster and acts as an unspecific repressor of transcription |
| title_full | The tardigrade Dsup protein enhances radioresistance in Drosophila melanogaster and acts as an unspecific repressor of transcription |
| title_fullStr | The tardigrade Dsup protein enhances radioresistance in Drosophila melanogaster and acts as an unspecific repressor of transcription |
| title_full_unstemmed | The tardigrade Dsup protein enhances radioresistance in Drosophila melanogaster and acts as an unspecific repressor of transcription |
| title_short | The tardigrade Dsup protein enhances radioresistance in Drosophila melanogaster and acts as an unspecific repressor of transcription |
| title_sort | tardigrade dsup protein enhances radioresistance in drosophila melanogaster and acts as an unspecific repressor of transcription |
| topic | Molecular biology Cell biology Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223010751 |
| work_keys_str_mv | AT mikhailzarubin thetardigradedsupproteinenhancesradioresistanceindrosophilamelanogasterandactsasanunspecificrepressoroftranscription AT talyanaazorskaya thetardigradedsupproteinenhancesradioresistanceindrosophilamelanogasterandactsasanunspecificrepressoroftranscription AT olgakuldoshina thetardigradedsupproteinenhancesradioresistanceindrosophilamelanogasterandactsasanunspecificrepressoroftranscription AT sergeyalekseev thetardigradedsupproteinenhancesradioresistanceindrosophilamelanogasterandactsasanunspecificrepressoroftranscription AT semenmitrofanov thetardigradedsupproteinenhancesradioresistanceindrosophilamelanogasterandactsasanunspecificrepressoroftranscription AT elenakravchenko thetardigradedsupproteinenhancesradioresistanceindrosophilamelanogasterandactsasanunspecificrepressoroftranscription AT mikhailzarubin tardigradedsupproteinenhancesradioresistanceindrosophilamelanogasterandactsasanunspecificrepressoroftranscription AT talyanaazorskaya tardigradedsupproteinenhancesradioresistanceindrosophilamelanogasterandactsasanunspecificrepressoroftranscription AT olgakuldoshina tardigradedsupproteinenhancesradioresistanceindrosophilamelanogasterandactsasanunspecificrepressoroftranscription AT sergeyalekseev tardigradedsupproteinenhancesradioresistanceindrosophilamelanogasterandactsasanunspecificrepressoroftranscription AT semenmitrofanov tardigradedsupproteinenhancesradioresistanceindrosophilamelanogasterandactsasanunspecificrepressoroftranscription AT elenakravchenko tardigradedsupproteinenhancesradioresistanceindrosophilamelanogasterandactsasanunspecificrepressoroftranscription |