Antimicrobial Resistance in <i>Escherichia coli</i> and Its Correlation with Antimicrobial Use on Commercial Poultry Farms in Bangladesh
Antimicrobial resistance is a global concern, posing risks to human and animal health. This research quantified antimicrobial resistance (AMR) in <i>E. coli</i> isolates from poultry fecal and environmental samples in Bangladesh and explored their association with antimicrobial use (AMU)...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/9/1361 |
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| author | Nelima Ibrahim Filip Boyen Md. Abu Shoieb Mohsin Moniek Ringenier Anna Catharina Berge Ilias Chantziaras Guillaume Fournié Dirk Pfeiffer Jeroen Dewulf |
| author_facet | Nelima Ibrahim Filip Boyen Md. Abu Shoieb Mohsin Moniek Ringenier Anna Catharina Berge Ilias Chantziaras Guillaume Fournié Dirk Pfeiffer Jeroen Dewulf |
| author_sort | Nelima Ibrahim |
| collection | DOAJ |
| description | Antimicrobial resistance is a global concern, posing risks to human and animal health. This research quantified antimicrobial resistance (AMR) in <i>E. coli</i> isolates from poultry fecal and environmental samples in Bangladesh and explored their association with antimicrobial use (AMU). We screened 725 fecal and 250 environmental samples from 94 conventional broilers and 51 Sonali farms for <i>E. coli</i> presence using MALDI-TOF mass spectrometry. AMU data were collected at flock levels, expressed as treatment incidence (TI), while minimum inhibitory concentrations (MIC) for 14 antibiotics were determined on five fecal <i>E. coli</i> isolates per farm and on all environmental isolates. MIC results were interpreted using human clinical breakpoints and EUCAST epidemiological cut-off values (ECOFFs). Acquired resistance against commonly used antimicrobial agents such as ciprofloxacin, tetracycline and ampicillin, was extremely high and predominantly clinically relevant. There was a moderate correlation between fecal and environmental antibiotic resistance index (ARI), but there was no significant correlation between AMU and AMR, suggesting that the observed AMR prevalence is unrelated to current AMU in poultry, but may be due to high historical AMU. A high level of multidrug resistance, including against critically important antimicrobials, was found in both farm types. Therefore, an AMR/AMU surveillance program is urgently needed in the poultry production sector of Bangladesh. |
| first_indexed | 2024-03-10T23:06:46Z |
| format | Article |
| id | doaj.art-b4bf7a887af045a9912435ff23b96b76 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T23:06:46Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-b4bf7a887af045a9912435ff23b96b762023-11-19T09:16:42ZengMDPI AGAntibiotics2079-63822023-08-01129136110.3390/antibiotics12091361Antimicrobial Resistance in <i>Escherichia coli</i> and Its Correlation with Antimicrobial Use on Commercial Poultry Farms in BangladeshNelima Ibrahim0Filip Boyen1Md. Abu Shoieb Mohsin2Moniek Ringenier3Anna Catharina Berge4Ilias Chantziaras5Guillaume Fournié6Dirk Pfeiffer7Jeroen Dewulf8Faculty of Veterinary Medicine, Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, BelgiumFaculty of Veterinary Medicine, Department of Pathobiology, Pharmacology and Zoological Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, BelgiumDepartment of Medicine and Surgery, Chattogram Veterinary and Animal Sciences University, Chattogram 4202, BangladeshFaculty of Veterinary Medicine, Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, BelgiumFaculty of Veterinary Medicine, Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, BelgiumFaculty of Veterinary Medicine, Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, BelgiumUniversité de Lyon, INRAE, VetAgro Sup, UMR EPIA, 69280 Marcy l’Etoile, FranceDepartment of Pathobiology and Population Sciences, Royal Veterinary College, Royal College Street, London NW1 0TU, UKFaculty of Veterinary Medicine, Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, BelgiumAntimicrobial resistance is a global concern, posing risks to human and animal health. This research quantified antimicrobial resistance (AMR) in <i>E. coli</i> isolates from poultry fecal and environmental samples in Bangladesh and explored their association with antimicrobial use (AMU). We screened 725 fecal and 250 environmental samples from 94 conventional broilers and 51 Sonali farms for <i>E. coli</i> presence using MALDI-TOF mass spectrometry. AMU data were collected at flock levels, expressed as treatment incidence (TI), while minimum inhibitory concentrations (MIC) for 14 antibiotics were determined on five fecal <i>E. coli</i> isolates per farm and on all environmental isolates. MIC results were interpreted using human clinical breakpoints and EUCAST epidemiological cut-off values (ECOFFs). Acquired resistance against commonly used antimicrobial agents such as ciprofloxacin, tetracycline and ampicillin, was extremely high and predominantly clinically relevant. There was a moderate correlation between fecal and environmental antibiotic resistance index (ARI), but there was no significant correlation between AMU and AMR, suggesting that the observed AMR prevalence is unrelated to current AMU in poultry, but may be due to high historical AMU. A high level of multidrug resistance, including against critically important antimicrobials, was found in both farm types. Therefore, an AMR/AMU surveillance program is urgently needed in the poultry production sector of Bangladesh.https://www.mdpi.com/2079-6382/12/9/1361MICfecal and environmental samplesECOFF valueCLSIbroilerSonali |
| spellingShingle | Nelima Ibrahim Filip Boyen Md. Abu Shoieb Mohsin Moniek Ringenier Anna Catharina Berge Ilias Chantziaras Guillaume Fournié Dirk Pfeiffer Jeroen Dewulf Antimicrobial Resistance in <i>Escherichia coli</i> and Its Correlation with Antimicrobial Use on Commercial Poultry Farms in Bangladesh Antibiotics MIC fecal and environmental samples ECOFF value CLSI broiler Sonali |
| title | Antimicrobial Resistance in <i>Escherichia coli</i> and Its Correlation with Antimicrobial Use on Commercial Poultry Farms in Bangladesh |
| title_full | Antimicrobial Resistance in <i>Escherichia coli</i> and Its Correlation with Antimicrobial Use on Commercial Poultry Farms in Bangladesh |
| title_fullStr | Antimicrobial Resistance in <i>Escherichia coli</i> and Its Correlation with Antimicrobial Use on Commercial Poultry Farms in Bangladesh |
| title_full_unstemmed | Antimicrobial Resistance in <i>Escherichia coli</i> and Its Correlation with Antimicrobial Use on Commercial Poultry Farms in Bangladesh |
| title_short | Antimicrobial Resistance in <i>Escherichia coli</i> and Its Correlation with Antimicrobial Use on Commercial Poultry Farms in Bangladesh |
| title_sort | antimicrobial resistance in i escherichia coli i and its correlation with antimicrobial use on commercial poultry farms in bangladesh |
| topic | MIC fecal and environmental samples ECOFF value CLSI broiler Sonali |
| url | https://www.mdpi.com/2079-6382/12/9/1361 |
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