Expression of the muscle-associated gene MYF6 in hairy cell leukemia.
Hairy cell leukemia (HCL) is a purine analog-responsive B-cell malignancy containing the BRAF V600E mutation, expressing CD22, CD11c, CD103, tartrate resistant acid phosphatase (TRAP) CD25, CD123, and annexin 1A. BRAF V600E and the latter 4 markers are usually absent in the more aggressive and chemo...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0227586 |
| _version_ | 1819260258122465280 |
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| author | Evgeny Arons Hong Zhou Mark Sokolsky Daniel Gorelik Katherine Potocka Sarah Davies Erin Fykes Katherine Still Daniel C Edelman Yonghong Wang Paul S Meltzer Mark Raffeld Adrian Wiestner Liqiang Xi Hao-Wei Wang Maryalice Stetler-Stevenson Constance Yuan Robert J Kreitman |
| author_facet | Evgeny Arons Hong Zhou Mark Sokolsky Daniel Gorelik Katherine Potocka Sarah Davies Erin Fykes Katherine Still Daniel C Edelman Yonghong Wang Paul S Meltzer Mark Raffeld Adrian Wiestner Liqiang Xi Hao-Wei Wang Maryalice Stetler-Stevenson Constance Yuan Robert J Kreitman |
| author_sort | Evgeny Arons |
| collection | DOAJ |
| description | Hairy cell leukemia (HCL) is a purine analog-responsive B-cell malignancy containing the BRAF V600E mutation, expressing CD22, CD11c, CD103, tartrate resistant acid phosphatase (TRAP) CD25, CD123, and annexin 1A. BRAF V600E and the latter 4 markers are usually absent in the more aggressive and chemoresistant variant HCLv. To evaluate differences between HCL and HCLv, expression microarrays comparing HCL with HCLv were performed for 24694 genes using 47323 probes. Microarray data from 35 HCL and 27 HCLv purified samples showed the greatest HCL-HCLv difference in the muscle-associated gene MYF6, expressed by its 2 probes 18.5- and 10.8-fold higher in HCL than HCLv (p<0.0001). By real-time quantitative PCR (RQ-PCR), 100% of 152 classic HCL samples were MYF6-positive, vs 5 (6%) of 90 blood donors. MYF6-expression was also detected in 18 (35%) of 51 with HCLv, 11 (92%) of 12 with HCL expressing unmutated IGHV4-34, 35 (73%) of 48 with chronic lymphocytic leukemia (CLL), and 1 (8%) of 12 with mantle cell lymphoma. Hypomethylation status of MYF6 supported expression in HCL more than HCLv. Posttreatment blood samples becoming negative by flow cytometry remained MYF6+ by RQ-PCR in 42 (48%) of 87 HCL patients, and MYF6 RQ-PCR could detect 1 HCL in 105 normal cells. MYF6, universally expressed in HCL and in most CLL samples, may be a useful biomarker for these leukemias. Further studies are underway to determine the role of MYF6 in HCL. |
| first_indexed | 2024-12-23T19:23:03Z |
| format | Article |
| id | doaj.art-b4c3619133ba44569ecd584f874b3177 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T19:23:03Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b4c3619133ba44569ecd584f874b31772022-12-21T17:34:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01152e022758610.1371/journal.pone.0227586Expression of the muscle-associated gene MYF6 in hairy cell leukemia.Evgeny AronsHong ZhouMark SokolskyDaniel GorelikKatherine PotockaSarah DaviesErin FykesKatherine StillDaniel C EdelmanYonghong WangPaul S MeltzerMark RaffeldAdrian WiestnerLiqiang XiHao-Wei WangMaryalice Stetler-StevensonConstance YuanRobert J KreitmanHairy cell leukemia (HCL) is a purine analog-responsive B-cell malignancy containing the BRAF V600E mutation, expressing CD22, CD11c, CD103, tartrate resistant acid phosphatase (TRAP) CD25, CD123, and annexin 1A. BRAF V600E and the latter 4 markers are usually absent in the more aggressive and chemoresistant variant HCLv. To evaluate differences between HCL and HCLv, expression microarrays comparing HCL with HCLv were performed for 24694 genes using 47323 probes. Microarray data from 35 HCL and 27 HCLv purified samples showed the greatest HCL-HCLv difference in the muscle-associated gene MYF6, expressed by its 2 probes 18.5- and 10.8-fold higher in HCL than HCLv (p<0.0001). By real-time quantitative PCR (RQ-PCR), 100% of 152 classic HCL samples were MYF6-positive, vs 5 (6%) of 90 blood donors. MYF6-expression was also detected in 18 (35%) of 51 with HCLv, 11 (92%) of 12 with HCL expressing unmutated IGHV4-34, 35 (73%) of 48 with chronic lymphocytic leukemia (CLL), and 1 (8%) of 12 with mantle cell lymphoma. Hypomethylation status of MYF6 supported expression in HCL more than HCLv. Posttreatment blood samples becoming negative by flow cytometry remained MYF6+ by RQ-PCR in 42 (48%) of 87 HCL patients, and MYF6 RQ-PCR could detect 1 HCL in 105 normal cells. MYF6, universally expressed in HCL and in most CLL samples, may be a useful biomarker for these leukemias. Further studies are underway to determine the role of MYF6 in HCL.https://doi.org/10.1371/journal.pone.0227586 |
| spellingShingle | Evgeny Arons Hong Zhou Mark Sokolsky Daniel Gorelik Katherine Potocka Sarah Davies Erin Fykes Katherine Still Daniel C Edelman Yonghong Wang Paul S Meltzer Mark Raffeld Adrian Wiestner Liqiang Xi Hao-Wei Wang Maryalice Stetler-Stevenson Constance Yuan Robert J Kreitman Expression of the muscle-associated gene MYF6 in hairy cell leukemia. PLoS ONE |
| title | Expression of the muscle-associated gene MYF6 in hairy cell leukemia. |
| title_full | Expression of the muscle-associated gene MYF6 in hairy cell leukemia. |
| title_fullStr | Expression of the muscle-associated gene MYF6 in hairy cell leukemia. |
| title_full_unstemmed | Expression of the muscle-associated gene MYF6 in hairy cell leukemia. |
| title_short | Expression of the muscle-associated gene MYF6 in hairy cell leukemia. |
| title_sort | expression of the muscle associated gene myf6 in hairy cell leukemia |
| url | https://doi.org/10.1371/journal.pone.0227586 |
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