Proteomic Analysis of the Predatory Venom of <i>Conus striatus</i> Reveals Novel and Population-Specific κA-Conotoxin SIVC
Animal venoms are a rich source of pharmacological compounds with ecological and evolutionary significance, as well as with therapeutic and biotechnological potentials. Among the most promising venomous animals, cone snails produce potent neurotoxic venom to facilitate prey capture and defend agains...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/14/11/799 |
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| author | Fabrice Saintmont Guillaume Cazals Claudia Bich Sebastien Dutertre |
| author_facet | Fabrice Saintmont Guillaume Cazals Claudia Bich Sebastien Dutertre |
| author_sort | Fabrice Saintmont |
| collection | DOAJ |
| description | Animal venoms are a rich source of pharmacological compounds with ecological and evolutionary significance, as well as with therapeutic and biotechnological potentials. Among the most promising venomous animals, cone snails produce potent neurotoxic venom to facilitate prey capture and defend against aggressors. <i>Conus striatus</i>, one of the largest piscivorous species, is widely distributed, from east African coasts to remote Polynesian Islands. In this study, we investigated potential intraspecific differences in venom composition between distinct geographical populations from Mayotte Island (Indian Ocean) and Australia (Pacific Ocean). Significant variations were noted among the most abundant components, namely the κA-conotoxins, which contain three disulfide bridges and complex glycosylations. The amino acid sequence of a novel κA-conotoxin SIVC, including its N-terminal acetylated variant, was deciphered using tandem mass spectrometry (MS/MS). In addition, the glycosylation pattern was found to be consisting of two HexNAc and four Hex for the Mayotte population, which diverge from the previously characterized two HexNAc and three Hex combinations for this species, collected elsewhere. Whereas the biological and ecological roles of these modifications remain to be investigated, population-specific glycosylation patterns provide, for the first time, a new level of intraspecific variations in cone snail venoms. |
| first_indexed | 2024-03-09T17:57:26Z |
| format | Article |
| id | doaj.art-b4c463e4d17d42e7bee434b38a8f2e37 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T17:57:26Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-b4c463e4d17d42e7bee434b38a8f2e372023-11-24T10:15:24ZengMDPI AGToxins2072-66512022-11-01141179910.3390/toxins14110799Proteomic Analysis of the Predatory Venom of <i>Conus striatus</i> Reveals Novel and Population-Specific κA-Conotoxin SIVCFabrice Saintmont0Guillaume Cazals1Claudia Bich2Sebastien Dutertre3IBMM, Université Montpellier, CNRS, ENSCM, 34093 Montpellier, FranceIBMM, Université Montpellier, CNRS, ENSCM, 34093 Montpellier, FranceIBMM, Université Montpellier, CNRS, ENSCM, 34093 Montpellier, FranceIBMM, Université Montpellier, CNRS, ENSCM, 34093 Montpellier, FranceAnimal venoms are a rich source of pharmacological compounds with ecological and evolutionary significance, as well as with therapeutic and biotechnological potentials. Among the most promising venomous animals, cone snails produce potent neurotoxic venom to facilitate prey capture and defend against aggressors. <i>Conus striatus</i>, one of the largest piscivorous species, is widely distributed, from east African coasts to remote Polynesian Islands. In this study, we investigated potential intraspecific differences in venom composition between distinct geographical populations from Mayotte Island (Indian Ocean) and Australia (Pacific Ocean). Significant variations were noted among the most abundant components, namely the κA-conotoxins, which contain three disulfide bridges and complex glycosylations. The amino acid sequence of a novel κA-conotoxin SIVC, including its N-terminal acetylated variant, was deciphered using tandem mass spectrometry (MS/MS). In addition, the glycosylation pattern was found to be consisting of two HexNAc and four Hex for the Mayotte population, which diverge from the previously characterized two HexNAc and three Hex combinations for this species, collected elsewhere. Whereas the biological and ecological roles of these modifications remain to be investigated, population-specific glycosylation patterns provide, for the first time, a new level of intraspecific variations in cone snail venoms.https://www.mdpi.com/2072-6651/14/11/799<i>Conus striatus</i>conotoxinglycosylationmass spectrometry |
| spellingShingle | Fabrice Saintmont Guillaume Cazals Claudia Bich Sebastien Dutertre Proteomic Analysis of the Predatory Venom of <i>Conus striatus</i> Reveals Novel and Population-Specific κA-Conotoxin SIVC Toxins <i>Conus striatus</i> conotoxin glycosylation mass spectrometry |
| title | Proteomic Analysis of the Predatory Venom of <i>Conus striatus</i> Reveals Novel and Population-Specific κA-Conotoxin SIVC |
| title_full | Proteomic Analysis of the Predatory Venom of <i>Conus striatus</i> Reveals Novel and Population-Specific κA-Conotoxin SIVC |
| title_fullStr | Proteomic Analysis of the Predatory Venom of <i>Conus striatus</i> Reveals Novel and Population-Specific κA-Conotoxin SIVC |
| title_full_unstemmed | Proteomic Analysis of the Predatory Venom of <i>Conus striatus</i> Reveals Novel and Population-Specific κA-Conotoxin SIVC |
| title_short | Proteomic Analysis of the Predatory Venom of <i>Conus striatus</i> Reveals Novel and Population-Specific κA-Conotoxin SIVC |
| title_sort | proteomic analysis of the predatory venom of i conus striatus i reveals novel and population specific κa conotoxin sivc |
| topic | <i>Conus striatus</i> conotoxin glycosylation mass spectrometry |
| url | https://www.mdpi.com/2072-6651/14/11/799 |
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