Surface‐Functionalizable Plant‐Derived Extracellular Vesicles for Targeted Drug Delivery Carrier Using Grapefruit
Abstract Recently, membrane‐modified mammalian exosomes have been considered strong candidates for targeted drug delivery carriers because of their biocompatibility, biodistribution, and low immune response. However, the widespread utilization of exosomes still requires overcoming several challengin...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2023-08-01
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| Series: | Advanced Materials Interfaces |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/admi.202300220 |
| _version_ | 1797752354324348928 |
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| author | Kyunghwan Moon Jihyeon Hur Kwang Pyo Kim Kangwon Lee Ji Yoon Kang |
| author_facet | Kyunghwan Moon Jihyeon Hur Kwang Pyo Kim Kangwon Lee Ji Yoon Kang |
| author_sort | Kyunghwan Moon |
| collection | DOAJ |
| description | Abstract Recently, membrane‐modified mammalian exosomes have been considered strong candidates for targeted drug delivery carriers because of their biocompatibility, biodistribution, and low immune response. However, the widespread utilization of exosomes still requires overcoming several challenging issues, including low stability, high production cost, and low mass productivity. Therefore, artificial extracellular vesicles (EVs) derived from cell membranes or liposomes containing various lipids have been suggested. However, only a few meet the demands of cost‐effective mass production and durability of EVs. Therefore, this study investigates the feasibility of replacing mammalian cell exosomes and liposomes with plant‐derived extracellular vesicles (pEVs) as targeted drug delivery carriers. They are characterized by nontoxicity, high stability, and high yield. Adding a functionalizable lipid moiety with a maleimide group at the membrane of grapefruit‐derived pEVs imparts targeting ability. The targeting function can be easily enhanced by attaching an aptamer using click chemistry. Indeed, treatment of brain cells with pEV‐aptamers (hCMEC/D3 and U87MG) confirms that aptamer functionalization of pEV enhanced selective cellular uptake. Functionalization of the pEV membrane using aptamer is expected to be effective in providing low‐cost and mass‐producible targeted drug delivery carriers with similar efficacy as mammalian exosomes or liposomes. |
| first_indexed | 2024-03-12T17:02:07Z |
| format | Article |
| id | doaj.art-b4c8a803a3174a3cbe8c0a567a5f21d9 |
| institution | Directory Open Access Journal |
| issn | 2196-7350 |
| language | English |
| last_indexed | 2024-03-12T17:02:07Z |
| publishDate | 2023-08-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Materials Interfaces |
| spelling | doaj.art-b4c8a803a3174a3cbe8c0a567a5f21d92023-08-07T10:10:02ZengWiley-VCHAdvanced Materials Interfaces2196-73502023-08-011022n/an/a10.1002/admi.202300220Surface‐Functionalizable Plant‐Derived Extracellular Vesicles for Targeted Drug Delivery Carrier Using GrapefruitKyunghwan Moon0Jihyeon Hur1Kwang Pyo Kim2Kangwon Lee3Ji Yoon Kang4Brain Science Institute Korea Institute of Science and Technology Seoul 02792 Republic of KoreaDepartment of Applied Chemistry Institute of Natural Science Global Center for Pharmaceutical Ingredient Materials Kyung Hee University Yongin 17104 Republic of KoreaDepartment of Applied Chemistry Institute of Natural Science Global Center for Pharmaceutical Ingredient Materials Kyung Hee University Yongin 17104 Republic of KoreaDepartment of Applied Bioengineering Graduate School of Convergence Science and Technology Seoul National University Seoul 08826 Republic of KoreaBrain Science Institute Korea Institute of Science and Technology Seoul 02792 Republic of KoreaAbstract Recently, membrane‐modified mammalian exosomes have been considered strong candidates for targeted drug delivery carriers because of their biocompatibility, biodistribution, and low immune response. However, the widespread utilization of exosomes still requires overcoming several challenging issues, including low stability, high production cost, and low mass productivity. Therefore, artificial extracellular vesicles (EVs) derived from cell membranes or liposomes containing various lipids have been suggested. However, only a few meet the demands of cost‐effective mass production and durability of EVs. Therefore, this study investigates the feasibility of replacing mammalian cell exosomes and liposomes with plant‐derived extracellular vesicles (pEVs) as targeted drug delivery carriers. They are characterized by nontoxicity, high stability, and high yield. Adding a functionalizable lipid moiety with a maleimide group at the membrane of grapefruit‐derived pEVs imparts targeting ability. The targeting function can be easily enhanced by attaching an aptamer using click chemistry. Indeed, treatment of brain cells with pEV‐aptamers (hCMEC/D3 and U87MG) confirms that aptamer functionalization of pEV enhanced selective cellular uptake. Functionalization of the pEV membrane using aptamer is expected to be effective in providing low‐cost and mass‐producible targeted drug delivery carriers with similar efficacy as mammalian exosomes or liposomes.https://doi.org/10.1002/admi.202300220drug delivery carrierplant‐derived extracellular vesicletargeted delivery |
| spellingShingle | Kyunghwan Moon Jihyeon Hur Kwang Pyo Kim Kangwon Lee Ji Yoon Kang Surface‐Functionalizable Plant‐Derived Extracellular Vesicles for Targeted Drug Delivery Carrier Using Grapefruit Advanced Materials Interfaces drug delivery carrier plant‐derived extracellular vesicle targeted delivery |
| title | Surface‐Functionalizable Plant‐Derived Extracellular Vesicles for Targeted Drug Delivery Carrier Using Grapefruit |
| title_full | Surface‐Functionalizable Plant‐Derived Extracellular Vesicles for Targeted Drug Delivery Carrier Using Grapefruit |
| title_fullStr | Surface‐Functionalizable Plant‐Derived Extracellular Vesicles for Targeted Drug Delivery Carrier Using Grapefruit |
| title_full_unstemmed | Surface‐Functionalizable Plant‐Derived Extracellular Vesicles for Targeted Drug Delivery Carrier Using Grapefruit |
| title_short | Surface‐Functionalizable Plant‐Derived Extracellular Vesicles for Targeted Drug Delivery Carrier Using Grapefruit |
| title_sort | surface functionalizable plant derived extracellular vesicles for targeted drug delivery carrier using grapefruit |
| topic | drug delivery carrier plant‐derived extracellular vesicle targeted delivery |
| url | https://doi.org/10.1002/admi.202300220 |
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