Inhibitory effect of isoamericanol A from Jatropha curcas seeds on the growth of MCF-7 human breast cancer cell line by G2/M cell cycle arrest
Although various parts of J. curcas (Jatropha curcas L., Euphorbiaceae) have long been used as traditional folk medicines for their antiviral, analgesic, and/or antidotal efficacies, we are the first to investigate the role of anti-carcinogenicity of isoamericanol A (IAA) from the seed extract. Our...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-01-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844015303686 |
| _version_ | 1818864384092405760 |
|---|---|
| author | Ayako Katagi Li Sui Kazuyo Kamitori Toshisada Suzuki Takeshi Katayama Akram Hossain Chisato Noguchi Youyi Dong Fuminori Yamaguchi Masaaki Tokuda |
| author_facet | Ayako Katagi Li Sui Kazuyo Kamitori Toshisada Suzuki Takeshi Katayama Akram Hossain Chisato Noguchi Youyi Dong Fuminori Yamaguchi Masaaki Tokuda |
| author_sort | Ayako Katagi |
| collection | DOAJ |
| description | Although various parts of J. curcas (Jatropha curcas L., Euphorbiaceae) have long been used as traditional folk medicines for their antiviral, analgesic, and/or antidotal efficacies, we are the first to investigate the role of anti-carcinogenicity of isoamericanol A (IAA) from the seed extract. Our results showed that IAA is capable of inhibiting cell proliferation in a dose-dependent manner on the human cancer cell lines of MCF-7, MDA-MB231, HuH-7, and HeLa. Flow cytometry analysis showed IAA significantly induces cell cycle arrest at G2/M on MCF-7 cells. At both protein and mRNA levels examined by western blot and real-time PCR, the results revealed increased expression of BTG2 (B-cell translocation gene 2), p21 (p21WAF1/CIPI), and GADD45A (growth arrest and DNA-damage-inducible, alpha) after IAA treatment, but inversed expression in CDK1 (cyclin-dependent kinase 1) and cyclins B1 and B2. All these effects contribute to G2/M cell cycle arrest. Furthermore, these results coincide with the changes in molecular expressions determined by DNA-microarray analysis. Our findings indicate that IAA has an inhibitory effect on cell proliferation of MCF-7 through cell cycle arrest, giving it great potential as a future therapeutic reagent for cancers. |
| first_indexed | 2024-12-19T10:30:48Z |
| format | Article |
| id | doaj.art-b4c97a5528414255b755c7e0ad417010 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-19T10:30:48Z |
| publishDate | 2016-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-b4c97a5528414255b755c7e0ad4170102022-12-21T20:25:46ZengElsevierHeliyon2405-84402016-01-012110.1016/j.heliyon.2015.e00055Inhibitory effect of isoamericanol A from Jatropha curcas seeds on the growth of MCF-7 human breast cancer cell line by G2/M cell cycle arrestAyako Katagi0Li Sui1Kazuyo Kamitori2Toshisada Suzuki3Takeshi Katayama4Akram Hossain5Chisato Noguchi6Youyi Dong7Fuminori Yamaguchi8Masaaki Tokuda9Department of Cell Physiology, Kagawa University, Faculty of Medicine / Graduate School of Medicine, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793 JapanDepartment of Cell Physiology, Kagawa University, Faculty of Medicine / Graduate School of Medicine, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793 JapanDepartment of Cell Physiology, Kagawa University, Faculty of Medicine / Graduate School of Medicine, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793 JapanLaboratory of Biomass Chemistry, Biological Molecular Chemistry, Kagawa University, Faculty of Agriculture / Graduate School of Agriculture, 2393 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793 JapanLaboratory of Biomass Chemistry, Biological Molecular Chemistry, Kagawa University, Faculty of Agriculture / Graduate School of Agriculture, 2393 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793 JapanDepartment of Cell Physiology, Kagawa University, Faculty of Medicine / Graduate School of Medicine, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793 JapanDepartment of Cell Physiology, Kagawa University, Faculty of Medicine / Graduate School of Medicine, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793 JapanDepartment of Cell Physiology, Kagawa University, Faculty of Medicine / Graduate School of Medicine, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793 JapanDepartment of Cell Physiology, Kagawa University, Faculty of Medicine / Graduate School of Medicine, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793 JapanDepartment of Cell Physiology, Kagawa University, Faculty of Medicine / Graduate School of Medicine, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793 JapanAlthough various parts of J. curcas (Jatropha curcas L., Euphorbiaceae) have long been used as traditional folk medicines for their antiviral, analgesic, and/or antidotal efficacies, we are the first to investigate the role of anti-carcinogenicity of isoamericanol A (IAA) from the seed extract. Our results showed that IAA is capable of inhibiting cell proliferation in a dose-dependent manner on the human cancer cell lines of MCF-7, MDA-MB231, HuH-7, and HeLa. Flow cytometry analysis showed IAA significantly induces cell cycle arrest at G2/M on MCF-7 cells. At both protein and mRNA levels examined by western blot and real-time PCR, the results revealed increased expression of BTG2 (B-cell translocation gene 2), p21 (p21WAF1/CIPI), and GADD45A (growth arrest and DNA-damage-inducible, alpha) after IAA treatment, but inversed expression in CDK1 (cyclin-dependent kinase 1) and cyclins B1 and B2. All these effects contribute to G2/M cell cycle arrest. Furthermore, these results coincide with the changes in molecular expressions determined by DNA-microarray analysis. Our findings indicate that IAA has an inhibitory effect on cell proliferation of MCF-7 through cell cycle arrest, giving it great potential as a future therapeutic reagent for cancers.http://www.sciencedirect.com/science/article/pii/S2405844015303686Health sciencesPharmaceutical sciencePlant biologyCancer treatmentCancer |
| spellingShingle | Ayako Katagi Li Sui Kazuyo Kamitori Toshisada Suzuki Takeshi Katayama Akram Hossain Chisato Noguchi Youyi Dong Fuminori Yamaguchi Masaaki Tokuda Inhibitory effect of isoamericanol A from Jatropha curcas seeds on the growth of MCF-7 human breast cancer cell line by G2/M cell cycle arrest Heliyon Health sciences Pharmaceutical science Plant biology Cancer treatment Cancer |
| title | Inhibitory effect of isoamericanol A from Jatropha curcas seeds on the growth of MCF-7 human breast cancer cell line by G2/M cell cycle arrest |
| title_full | Inhibitory effect of isoamericanol A from Jatropha curcas seeds on the growth of MCF-7 human breast cancer cell line by G2/M cell cycle arrest |
| title_fullStr | Inhibitory effect of isoamericanol A from Jatropha curcas seeds on the growth of MCF-7 human breast cancer cell line by G2/M cell cycle arrest |
| title_full_unstemmed | Inhibitory effect of isoamericanol A from Jatropha curcas seeds on the growth of MCF-7 human breast cancer cell line by G2/M cell cycle arrest |
| title_short | Inhibitory effect of isoamericanol A from Jatropha curcas seeds on the growth of MCF-7 human breast cancer cell line by G2/M cell cycle arrest |
| title_sort | inhibitory effect of isoamericanol a from jatropha curcas seeds on the growth of mcf 7 human breast cancer cell line by g2 m cell cycle arrest |
| topic | Health sciences Pharmaceutical science Plant biology Cancer treatment Cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2405844015303686 |
| work_keys_str_mv | AT ayakokatagi inhibitoryeffectofisoamericanolafromjatrophacurcasseedsonthegrowthofmcf7humanbreastcancercelllinebyg2mcellcyclearrest AT lisui inhibitoryeffectofisoamericanolafromjatrophacurcasseedsonthegrowthofmcf7humanbreastcancercelllinebyg2mcellcyclearrest AT kazuyokamitori inhibitoryeffectofisoamericanolafromjatrophacurcasseedsonthegrowthofmcf7humanbreastcancercelllinebyg2mcellcyclearrest AT toshisadasuzuki inhibitoryeffectofisoamericanolafromjatrophacurcasseedsonthegrowthofmcf7humanbreastcancercelllinebyg2mcellcyclearrest AT takeshikatayama inhibitoryeffectofisoamericanolafromjatrophacurcasseedsonthegrowthofmcf7humanbreastcancercelllinebyg2mcellcyclearrest AT akramhossain inhibitoryeffectofisoamericanolafromjatrophacurcasseedsonthegrowthofmcf7humanbreastcancercelllinebyg2mcellcyclearrest AT chisatonoguchi inhibitoryeffectofisoamericanolafromjatrophacurcasseedsonthegrowthofmcf7humanbreastcancercelllinebyg2mcellcyclearrest AT youyidong inhibitoryeffectofisoamericanolafromjatrophacurcasseedsonthegrowthofmcf7humanbreastcancercelllinebyg2mcellcyclearrest AT fuminoriyamaguchi inhibitoryeffectofisoamericanolafromjatrophacurcasseedsonthegrowthofmcf7humanbreastcancercelllinebyg2mcellcyclearrest AT masaakitokuda inhibitoryeffectofisoamericanolafromjatrophacurcasseedsonthegrowthofmcf7humanbreastcancercelllinebyg2mcellcyclearrest |