AVALIAÇÃO FENOTÍPICA E GENOTÍPICA DO PERFIL DE RESISTÊNCIA DE AMOSTRAS DE Staphylococcus aureus ISOLADAS DE CULTURAS CLÍNICAS E DE VIGILÂNCIA DE UM HOSPITAL DE ENSINO BRASILEIRO
Staphylococcus aureus can cause a variety of infections, especially nosocomial. Its importance lies in the combination of virulence, invasiveness and antibiotic resistance constituting therapeutic challenges. This study aimed to assess the genotype and phenotypic resistance profiles of samples o...
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| Format: | Article |
| Language: | Portuguese |
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Universidade do Oeste Paulista
2012-12-01
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| Series: | Colloquium Vitae |
| Subjects: | |
| Online Access: | http://revistas.unoeste.br/revistas/ojs/index.php/cv/article/view/804/1040 |
| _version_ | 1811287260626157568 |
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| author | Lívia Cafundó Almeida Marcus Vinicius Pimenta-Rodrigues Daniela Vanessa Moris Carlos Magno Castelo Branco Fortaleza Maria de Lourdes Ribeiro de Souza da Cunha |
| author_facet | Lívia Cafundó Almeida Marcus Vinicius Pimenta-Rodrigues Daniela Vanessa Moris Carlos Magno Castelo Branco Fortaleza Maria de Lourdes Ribeiro de Souza da Cunha |
| author_sort | Lívia Cafundó Almeida |
| collection | DOAJ |
| description | Staphylococcus aureus can cause a variety of infections, especially nosocomial. Its importance lies in the
combination of virulence, invasiveness and antibiotic resistance constituting therapeutic challenges. This
study aimed to assess the genotype and phenotypic resistance profiles of samples of S. aureus isolated from
hospitalized patients in a Brazilian teaching hospital. We evaluated 1078 samples obtained from surveillance
cultures and clinical S. aureus in hospitalized patients. To assess the phenotypic resistance profile was used
disk diffusion method according to CLSI criteria, 2011. For the determination of genotypic resistance was the
presence of the mecA gene by polymerase chain reaction - PCR. Of the 1078 samples tested
phenotypically, it was observed that 75.1% of the samples were Methicillin Resistant Staphylococcus aureus
(MRSA), and of these, 98.4% were resistant to oxacillin and 100%, cefoxitin. To determine genotype was
performed for the PCR amplification of the mecA gene. Of the 443 samples tested for the mecA 336 samples
were positive (75.8%). Of these samples, 85.7% showed resistance phenotype oxacillin and cefoxitin 88.4%.
Due to the high rate of MRSA, concludes the need for investment in research, rational use of antimicrobials
and creation of reference laboratories for verification of antimicrobial resistance. |
| first_indexed | 2024-04-13T03:15:33Z |
| format | Article |
| id | doaj.art-b4cbd65b800b46168dc5a0ca47e7ef76 |
| institution | Directory Open Access Journal |
| issn | 1984-6436 |
| language | Portuguese |
| last_indexed | 2024-04-13T03:15:33Z |
| publishDate | 2012-12-01 |
| publisher | Universidade do Oeste Paulista |
| record_format | Article |
| series | Colloquium Vitae |
| spelling | doaj.art-b4cbd65b800b46168dc5a0ca47e7ef762022-12-22T03:04:56ZporUniversidade do Oeste PaulistaColloquium Vitae1984-64362012-12-0142687810.5747/cv.2012.v004.n2.v063cAVALIAÇÃO FENOTÍPICA E GENOTÍPICA DO PERFIL DE RESISTÊNCIA DE AMOSTRAS DE Staphylococcus aureus ISOLADAS DE CULTURAS CLÍNICAS E DE VIGILÂNCIA DE UM HOSPITAL DE ENSINO BRASILEIROLívia Cafundó Almeida0Marcus Vinicius Pimenta-Rodrigues1 Daniela Vanessa Moris2Carlos Magno Castelo Branco Fortaleza3Maria de Lourdes Ribeiro de Souza da Cunha4University of Oeste PaulistaUniversity of Oeste Paulistaoris1University of Oeste PaulistaInstituto de Biociências de Botucatu – IBBFaculdade de Medicina de Botucatu – FMB da UNESP, BotucatuStaphylococcus aureus can cause a variety of infections, especially nosocomial. Its importance lies in the combination of virulence, invasiveness and antibiotic resistance constituting therapeutic challenges. This study aimed to assess the genotype and phenotypic resistance profiles of samples of S. aureus isolated from hospitalized patients in a Brazilian teaching hospital. We evaluated 1078 samples obtained from surveillance cultures and clinical S. aureus in hospitalized patients. To assess the phenotypic resistance profile was used disk diffusion method according to CLSI criteria, 2011. For the determination of genotypic resistance was the presence of the mecA gene by polymerase chain reaction - PCR. Of the 1078 samples tested phenotypically, it was observed that 75.1% of the samples were Methicillin Resistant Staphylococcus aureus (MRSA), and of these, 98.4% were resistant to oxacillin and 100%, cefoxitin. To determine genotype was performed for the PCR amplification of the mecA gene. Of the 443 samples tested for the mecA 336 samples were positive (75.8%). Of these samples, 85.7% showed resistance phenotype oxacillin and cefoxitin 88.4%. Due to the high rate of MRSA, concludes the need for investment in research, rational use of antimicrobials and creation of reference laboratories for verification of antimicrobial resistance.http://revistas.unoeste.br/revistas/ojs/index.php/cv/article/view/804/1040Staphylococcus aureusresistancemecA |
| spellingShingle | Lívia Cafundó Almeida Marcus Vinicius Pimenta-Rodrigues Daniela Vanessa Moris Carlos Magno Castelo Branco Fortaleza Maria de Lourdes Ribeiro de Souza da Cunha AVALIAÇÃO FENOTÍPICA E GENOTÍPICA DO PERFIL DE RESISTÊNCIA DE AMOSTRAS DE Staphylococcus aureus ISOLADAS DE CULTURAS CLÍNICAS E DE VIGILÂNCIA DE UM HOSPITAL DE ENSINO BRASILEIRO Colloquium Vitae Staphylococcus aureus resistance mecA |
| title | AVALIAÇÃO FENOTÍPICA E GENOTÍPICA DO PERFIL DE RESISTÊNCIA DE AMOSTRAS DE Staphylococcus aureus ISOLADAS DE CULTURAS CLÍNICAS E DE VIGILÂNCIA DE UM HOSPITAL DE ENSINO BRASILEIRO |
| title_full | AVALIAÇÃO FENOTÍPICA E GENOTÍPICA DO PERFIL DE RESISTÊNCIA DE AMOSTRAS DE Staphylococcus aureus ISOLADAS DE CULTURAS CLÍNICAS E DE VIGILÂNCIA DE UM HOSPITAL DE ENSINO BRASILEIRO |
| title_fullStr | AVALIAÇÃO FENOTÍPICA E GENOTÍPICA DO PERFIL DE RESISTÊNCIA DE AMOSTRAS DE Staphylococcus aureus ISOLADAS DE CULTURAS CLÍNICAS E DE VIGILÂNCIA DE UM HOSPITAL DE ENSINO BRASILEIRO |
| title_full_unstemmed | AVALIAÇÃO FENOTÍPICA E GENOTÍPICA DO PERFIL DE RESISTÊNCIA DE AMOSTRAS DE Staphylococcus aureus ISOLADAS DE CULTURAS CLÍNICAS E DE VIGILÂNCIA DE UM HOSPITAL DE ENSINO BRASILEIRO |
| title_short | AVALIAÇÃO FENOTÍPICA E GENOTÍPICA DO PERFIL DE RESISTÊNCIA DE AMOSTRAS DE Staphylococcus aureus ISOLADAS DE CULTURAS CLÍNICAS E DE VIGILÂNCIA DE UM HOSPITAL DE ENSINO BRASILEIRO |
| title_sort | avaliacao fenotipica e genotipica do perfil de resistencia de amostras de staphylococcus aureus isoladas de culturas clinicas e de vigilancia de um hospital de ensino brasileiro |
| topic | Staphylococcus aureus resistance mecA |
| url | http://revistas.unoeste.br/revistas/ojs/index.php/cv/article/view/804/1040 |
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